Biological variation of routine haematology and biochemistry measurands in the horse

Wright, Megan E.; Croser, Emma; Raidal, Sharanne; Baral, Randolph M.; Robinson, Wayne; Lievaart, Jan; Freeman, Kathleen

doi:10.1111/evj.13017

Cited by 14 publications

(7 citation statements)

References 25 publications

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“…Complete blood cell counts and biochemistry parameters remained within published reference ranges (17). While absolute counts were within accepted reference ranges, there was a decrease in numbers of neutrophils (median before 3.77 ×10 3 uL range Neutrophil numbers returned to baseline values by 24 h posttreatment.…”

Section: Resultsmentioning

confidence: 58%

“…Complete blood cell counts and biochemistry parameters remained within published reference ranges ( 17 ). While absolute counts were within accepted reference ranges, there was a decrease in numbers of neutrophils (median before 3.77 ×10 3 uL range 2.25–5.88, median after 2.19 range 2.11–5.12) and platelets (median before 130 ×10 3 uL range 132–140, median after 93 range 80–143).…”

Section: Resultsmentioning

confidence: 69%

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Feasibility of hemoperfusion using extracorporeal therapy in the horse

Hobbs,

Le Sueur,

Burke

et al. 2024

Front. Vet. Sci.

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ObjectiveDevelop, implement, and monitor for adverse effects of, a novel hemoperfusion therapy in adult horses.MethodsA prospective, observational feasibility study using three healthy adult horses from the North Carolina State University teaching herd. Health status was determined by physical exam, complete blood count, coagulation panel, and serum biochemistry. Each horse was instrumented with a 14 Fr × 25 cm double-lumen temporary hemodialysis catheter and underwent a 240 min polymer-based hemoperfusion session. Horses were administered unfractionated heparin to maintain anti-coagulation during the session. Given the novelty of this therapy in horses, each horse was treated as a learning opportunity that informed an iterative process of protocol development and modification.Measurements and main resultsOur long-term goal is to investigate potential clinical applications of hemoperfusion in horses, including cytokine reduction in horses with severe SIRS/sepsis. Horses were monitored for changes in clinical exam, biochemistry and hematology parameters. Additionally, cytokines were quantified to determine whether extracorporeal hemadsorption therapy alone caused an inflammatory response. Our results show that hemoperfusion therapy was associated with decreased platelet counts and serum albumin concentration. There was no significant change in plasma cytokine concentrations with hemoperfusion therapy. In one horse, the cytokine concentrations decreased, as previously reported with hemoperfusion therapy in humans.HypothesisWe hypothesized that hemoperfusion therapy could be performed in healthy adult horses without significant adverse effects.ConclusionPolymer-based hemoperfusion is a feasible extracorporeal therapy (ECT) modality for adult horses. Additional studies are needed to further establish clinical protocols, as well as establish efficacy of polymer-based hemoperfusion for treatment of various conditions in horses, including intoxications, immune-mediated conditions, and sepsis.

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Section: Resultsmentioning

confidence: 58%

“…Complete blood cell counts and biochemistry parameters remained within published reference ranges ( 17 ). While absolute counts were within accepted reference ranges, there was a decrease in numbers of neutrophils (median before 3.77 ×10 3 uL range 2.25–5.88, median after 2.19 range 2.11–5.12) and platelets (median before 130 ×10 3 uL range 132–140, median after 93 range 80–143).…”

Section: Resultsmentioning

confidence: 69%

Feasibility of hemoperfusion using extracorporeal therapy in the horse

Hobbs,

Le Sueur,

Burke

et al. 2024

Front. Vet. Sci.

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“…When spermatozoa are extended in currently available commercial media, these germ cells are exposed to supraphysiological glucose concentrations. Physiological glucose in serum in horses is 5 mM (Wright et al, 2019) while oviductal glucose concentrations are much lower, with maximal concentrations reported as being 300 μM (Campbell et al, 1979).…”

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confidence: 99%

An integrated overview on the regulation of sperm metabolism (glycolysis-Krebs cycle-oxidative phosphorylation)

Peña

Ortiz-Rodríguez

Gaitskell‐Phillips

et al. 2022

Animal Reproduction Science

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“…Most studies evaluating the biological variation were focused on domestic animal species such as dogs [ 13 , 14 ], cats [ 15 , 16 ], horses [ 17 ], and cows [ 18 ]. Furthermore, biological variation has also been evaluated in some non-domestic species, both mammals and non-mammals, due to the small number of reference individuals needed for the calculation [ 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Variation and Reference Change Value of Routine Hematology Measurands in a Population of Managed Bottlenose Dolphins (Tursiops truncatus)

Bonsembiante

Guglielmini

Berlanda

et al. 2023

Animals

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Hematological analyses are particularly useful in assessing a dolphin’s health status. However, the creation of appropriate reference intervals for this species is difficult due to the low number of reference individuals. The implementation of individual reference intervals (iRIs) allows researchers to overcome this limitation and, moreover, also consider the within-individual variability. The aims of this study were (1) to evaluate the biological variations in some hematological measurands, including erythrocytes (RBC), hematocrit (Hct), mean cellular volume and hemoglobin content (MCV and MCHC, respectively), RBC distribution width (RDW), leukocytes (WBC), and platelets (PLT); and (2) to calculate the index of individuality (IoI) and reference change value (RCV), which enable the production of iRIs, in healthy managed bottlenose dolphins. Seven dolphins were included, and the results of six hematological exams were analyzed for each animal. Analytical imprecision (CVa), within-dolphin variation (CVi), and between-dolphins variations (CVg) were calculated, and the IoI and RCV were derived for each measurand. All the hematological measurands had intermediate IoI except WBC, for which Iol was low. The calculated RCV ranged from 10.33% (MCV) to 186.51% (WBC). The results reveal that the majority of hematological measurands have an intermediate level of individuality in dolphins, and thus the application of iRIs is appropriate. The calculated RCV can also be applied to other managed dolphins and could be useful in interpreting serial CBC exams.

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Biological variation of routine haematology and biochemistry measurands in the horse

Abstract: The use of individualised reference intervals is justified for many measurands in horses, supporting the use of serial sampling, consideration of biological variation and application of reference change values for improved clinical decision making and patient management in equine practice.

Cited by 14 publications

References 25 publications

Feasibility of hemoperfusion using extracorporeal therapy in the horse

Feasibility of hemoperfusion using extracorporeal therapy in the horse

An integrated overview on the regulation of sperm metabolism (glycolysis-Krebs cycle-oxidative phosphorylation)

Biological Variation and Reference Change Value of Routine Hematology Measurands in a Population of Managed Bottlenose Dolphins (Tursiops truncatus)

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