Clinical Impact of Highly Condensed Stromal Vascular Fraction Injection in Surgical Management of Depressed and Contracted Scars

Lee, Junwon; Park, Seong Hoon; Lee, Seong Joo; Kim, Seong Hwan; Suh, In Suck; Jeong, Hii Sun

doi:10.1007/s00266-018-1216-9

Cited by 31 publications

(46 citation statements)

References 31 publications

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“…10 SVF has been applied for the treatment of scar-related conditions, given its regenerative potential, including the release of growth factors and activation of dermal angiogenesis. 11 Koh et al 12 postulated that enriching the vascular supply with co-implantation of SVF might improve the microenvironment and survival of the graft. In addition, the survival rates of autologous fat grafts range from 24% to 51.4%, whereas those of fat grafts enriched with a freshly isolated SVF are higher (63% to 90.4%) than those of traditional lipofilling at 12-19 months of follow-up.…”

Section: Introductionmentioning

confidence: 99%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Background: Cell-assisted lipotransfer (CAL) promotes the survival of fat grafts with high vascular density and improves skin quality by increasing collagen content. However, no study has quantified the changes on the skin surface, and rigorous methodological evaluations are still lacking. Design: Fifty patients were recruited and randomly divided into two groups: an experimental group (n = 25) that underwent a stromal vascular fraction (SVF)-assisted fat graft and a control group (n = 25) that underwent fat graft only. Methods: The SVF cells were counted, tested in terms of viability, and characterized. The volumes of whole faces were determined by using a 3D scanner and Geomagic software preoperation, immediately after surgery, and 6 months postoperation. Facial skin qualities, including spots, wrinkles, texture, pores, UV spots, brown spots, red areas, and porphyrins, were detected by a VISIA skin detector preoperation and 6 months postoperation. A visual analog scale was used for clinical evaluation. Results: The cell pellet contained 1-3 × 10 7 /mL of fresh SVF cells. The cell viability exceeded 98%. The immunophenotyping characteristics and stemness were consistent with the features of adipose-derived stem cells (ADSCs). The survival rate of SVF-enriched fat grafts was significantly higher than that of control grafts: 77.6% ±11.6% versus 56.2% ±9.5% (p < 0.001). The VISIA values of wrinkles (19.3 ± 6.6 versus 10.9 ± 5.5, p < 0.001) and texture (15.8 ± 7.0 versus 10.3 ± 5.0, p < 0.01) were significantly higher in SVF-enriched group than in control group at 6 months postoperation. During long-term follow-up, the majority of patients in both groups were satisfied with the final facial esthetic results.

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Section: Introductionmentioning

confidence: 99%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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“…The most commonly used procedures were enzymatic SVF isolation [ 18 – 21 , 25 , 28 , 30 , 31 ] and nano-fat [ 22 – 24 , 26 , 27 , 29 , 32 – 34 ], while Wu et al [ 17 ] used spectroscopy for SVF isolation. The fatty tissue was harvested from the abdomen or multiple sites, including flanks, hips, or thighs.…”

Section: Resultsmentioning

confidence: 99%

“…Most commonly (6 studies) they included FT digestion with collagenase and subsequent centrifugation. Lee et al [ 12 , 28 ] condensed the fat prior to enzyme addition. Zhou et al [ 21 ] cultured AD-MSC fraction after isolation and eventually produced a cell-free medium, rich in GFs and cytokines, later used in the study as the bioactive scaffold.…”

Section: Resultsmentioning

confidence: 99%

“…The studies analyzed have been represented by case reports ( n = 3), case series ( n = 7), case-controlled studies ( n = 2) [ 18 , 28 ], and prospective studies ( n = 7), as analyzed in the supplemental material section detailing.…”

Section: Resultsmentioning

confidence: 99%

“…Centrifuged again at 3000 rpm for 3 min N/A Condensed nano-fat Intradermally, after 18G needle is introduced to break underlying adhesions of the scar. Volume restored sub-dermally with fat graft combined with condensed nano-fat through a blunt 1.2-mm cannula 29G needle/1.2 mm blunt cannula 21–62 YO, mean 38.25 YO 3 to 26 years (mean formation time, 7.45 years) 6 months Lee et al [ 28 ] Site : abdomen Infiltration : N/A Technique : 3-mm blunt-tipped cannula. Liposuction kit used (Lipokit) Negative pressure : N/A Volume : 50 ml 1.…”

Section: Methodsmentioning

confidence: 99%

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Systematic review: Advances of fat tissue engineering as bioactive scaffold, bioactive material, and source for adipose-derived mesenchymal stem cells in wound and scar treatment

Gentile

Sterodimas

Calabrese³

et al. 2021

Stem Cell Res Ther

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Fat tissue (FT) has been used for many years in regenerative surgery as a bioactive material through the lipofilling/fat graft (F-GRF)–nano-fat technique, as a bioactive scaffold when it was enriched with adipose-derived mesenchymal stem cells (AD-MSCs) contained in the stromal vascular fraction (SVF), and as a direct source of AD-MSCs used in wound healing (WH) and scar treatment (ST). This systematic review aims to describe the advances in FT engineering applied to regenerative surgery (from bench to clinic), through the use of AD-MSCs, SVF contained in F-GRF in WH and ST. The work has been performed by assessing in the selected studies autologous graft of AD-MSCs, SVF, and F-GRF compared to any control for ST and WH. The protocol was developed following the Preferred Reporting for Items for Systematic Reviews and Meta-Analyses-Protocols (PRISMA-P) guidelines. A multistep search of the PubMed, MEDLINE, Embase, PreMEDLINE, Ebase, CINAHL, PsycINFO, Clinicaltrials.gov, Scopus database, and Cochrane databases has been performed to identify papers on AD-MSCs, SVF, and F-GRF use in WH and ST in which FT was used as bioactive material–scaffold and source of AD-MSCs. Of the 714 articles initially identified, 453 articles focusing on regenerative strategies in WH and ST were selected and, consequently, only 84 articles that apparently related to AD-MSC, SVF, and F-GRF were analyzed. Of these, 61 articles identified as pre-clinical, experimental, and in vitro, and 5 articles identified as a comment and systematic review were excluded. Only 18 original articles which strictly and exclusively focused on autologous AD-MSCs, SVF, and F-GRF in ST and WH were analyzed. The included studies had to match predetermined criteria according to the PICOS (patients, intervention, comparator, outcomes, and study design) approach. The identified studies described microscopic and clinical outcomes in patients treated with AD-MSCs, SVF, and F-GRF. Collected data confirmed the safety and efficacy of FT both as bioactive material–scaffold and source of AD-MSCs in WH and ST without major side effects. Graphical abstract

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Adipose‐derived stromal cells for nonhealing wounds: Emerging opportunities and challenges

Deptuła

Brzezicka

Skoniecka

et al. 2021

Medicinal Research Reviews

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Wound healing complications affect thousands of people each year, thus constituting a profound economic and medical burden. Chronic wounds are a highly complex problem that usually affects elderly patients as well as patients with comorbidities such as diabetes, cancer (surgery, radiotherapy/chemotherapy) or autoimmune diseases. Currently available methods of their treatment are not fully effective, so new solutions are constantly being sought. Cell‐based therapies seem to have great potential for use in stimulating wound healing. In recent years, much effort has been focused on characterizing of adipose‐derived mesenchymal stromal cells (AD‐MSCs) and evaluating their clinical use in regenerative medicine and other medical fields. These cells are easily obtained in large amounts from adipose tissue and show a high proregenerative potential, mainly through paracrine activities. In this review, the process of healing acute and nonhealing (chronic) wounds is detailed, with a special attention paid to the wounds of patients with diabetes and cancer. In addition, the methods and technical aspects of AD‐MSCs isolation, culture and transplantation in chronic wounds are described, and the characteristics, genetic stability and role of AD‐MSCs in wound healing are also summarized. The biological properties of AD‐MSCs isolated from subcutaneous and visceral adipose tissue are compared. Additionally, methods to increase their therapeutic potential as well as factors that may affect their biological functions are summarized. Finally, their therapeutic potential in the treatment of diabetic and oncological wounds is also discussed.

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Clinical Impact of Highly Condensed Stromal Vascular Fraction Injection in Surgical Management of Depressed and Contracted Scars

Abstract: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Cited by 31 publications

References 31 publications

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Systematic review: Advances of fat tissue engineering as bioactive scaffold, bioactive material, and source for adipose-derived mesenchymal stem cells in wound and scar treatment

Adipose‐derived stromal cells for nonhealing wounds: Emerging opportunities and challenges

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