The somatic mobilization of transposable element mariner-Mos1 during the Drosophila lifespan and its biological consequences

Pereira, Camila Moura; Stoffel, Tailini Jordana Reinehr; Callegari-Jacques, Sídia Maria; Hua-Van, Aurélie; Capy, Pierre; Loreto, Élgion Lúcio Silva

doi:10.1016/j.gene.2018.08.079

Cited by 10 publications

(15 citation statements)

References 31 publications

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“…Almost all studies on somatic transposition correlate this event with underlying diseases such as cancer [ 41 ] and Alzheimer’s disease [ 42 ], or ageing [ 43 , 44 , 45 ]. However, the expression [ 46 ] and excision [ 35 ] of TEs in normal somatic cells have already been described. In contrast, little is known about the somatic insertion of TEs, especially since this is one of the most important steps of the TS.…”

Section: Discussionmentioning

confidence: 99%

“…In agreement with our results, the somatic excision rates already found for the same D. simulans Wpcht strain are high. About 4% of fly cells showed non-autonomous copy excision at 20 °C and about 7% of flies maintained at 28 °C [ 35 ]. Furthermore, somatic excision is cumulative throughout development and also occurs in adult individuals [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, other stressors, such as UV radiation, do not lead to a similar increase [ 34 ]. Somatic excision of mariner occurs during all developmental stages of the Drosophila lifecycle and is cumulative [ 35 ]. Using RT-qPCR (real-time quantitative PCR), it was estimated that in a strain carrying one autonomous copy of mariner and the non-autonomous white-peach copy, approximately 4% of the mariner white-peach copies are excised from the white locus in adult flies when the flies are maintained at 20 °C, and 7% when maintained at 28 °C [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Somatic Mobilization: High Somatic Insertion Rate of mariner Transposable Element in Drosophila simulans

Cancian

Gomes

Loreto

2022

Insects

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Although transposable elements (TEs) are usually silent in somatic tissues, they are sometimes mobilized in the soma and can potentially have biological consequences. The mariner element is one of the TEs involved in somatic mobilization (SM) in Drosophila and has a high rate of somatic excision. It is also known that temperature is an important factor in the increase of the mariner element SM in the fly. However, it is important to emphasize that excision is only one step of TE transposition, and the final step in this process is insertion. In the present study, we used an assay based on sequencing of the mariner flanking region and developed a pipeline to identify novel mariner insertions in Drosophila simulans at 20 and 28 °C. We found that flies carrying two mariner copies (one autonomous and one non-autonomous) had an average of 236.4 (±99.3) to 279 (±107.7) new somatic insertions at 20 °C and an average of 172.7 (±95.3) to 252.6 (±67.3) at 28 °C. In addition, we detected fragments containing mariner and others without mariner in the same regions with low-coverage long-read sequencing, indicating the process of excision and insertion. In conclusion, a low number of autonomous copies of the mariner transposon can promote a high rate of new somatic insertions during the developmental stages of Drosophila. Additionally, the developed method seems to be sensitive and adequate for the verification and estimation of somatic insertion.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Somatic Mobilization: High Somatic Insertion Rate of mariner Transposable Element in Drosophila simulans

Cancian

Gomes

Loreto

2022

Insects

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“…One element of TE targeting comes directly from the fact that integration is restricted to open chromatin regions. This is apparent in mammalian brains, where somatic LINE insertions are enriched in the proximity of neuronal genes [11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33]. If we consider that TEs can be the target of epigenetic marks, the non-random and targeted tissue-specific distribution of TEs can be considered a mechanism to genetically fix a landmark, which could induce an epigenetic control of nearby gene expression (Figure 1B).…”

Section: Transposable Elements As An Essential Part Of Gene Expresmentioning

confidence: 99%

“…Somatic transpositions have also been receiving much attention, as a growing body of evidence is accumulating that associates detrimental biological effects with the somatic transposition of TE. One of the most interesting examples of this is represented by the mariner-Mos1 element of Drosophila (Class II transposon), which can be transposed during the Drosophila life cycle and negatively affects behavioral activities and embryonic viability [14]. Probably one of the most characterized pathological states linked to a new wave of transpositions in somatic cells is cancer, including ovarian [15], colorectal [16], and Fanconi anemia [17] cancer, to name just a few.…”

Section: Introductionmentioning

confidence: 99%

Roles of Transposable Elements in the Different Layers of Gene Expression Regulation

Drongitis

Aniello

Fucci

et al. 2019

IJMS

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The biology of transposable elements (TEs) is a fascinating and complex field of investigation. TEs represent a substantial fraction of many eukaryotic genomes and can influence many aspects of DNA function that range from the evolution of genetic information to duplication, stability, and gene expression. Their ability to move inside the genome has been largely recognized as a double-edged sword, as both useful and deleterious effects can result. A fundamental role has been played by the evolution of the molecular processes needed to properly control the expression of TEs. Today, we are far removed from the original reductive vision of TEs as “junk DNA”, and are more convinced that TEs represent an essential element in the regulation of gene expression. In this review, we summarize some of the more recent findings, mainly in the animal kingdom, concerning the active roles that TEs play at every level of gene expression regulation, including chromatin modification, splicing, and protein translation.

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Cyclophosphamide in Drosophila promotes genes and transposable elements differential expression and mitochondrial dysfunction

Stoffel

Segatto

Silva

et al. 2020

Comparative Biochemistry and Physiology Part C: Toxicology &

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Cyclophosphamide (CPA) is an alkylating agent used for cancer chemotherapy, organ transplantation, and autoimmune disease treatment. Here, mRNA sequencing and high-resolution respirometry were performed to evaluate the alterations of Drosophila melanogaster gene expression fed with CPA under acute (0.1 mg/mL, for 24 h) and chronic (0.05 mg/mL, for 35 days) treatments. Differential expression analysis was performed using Cufflinks-Cuffdiff, DESeq2, and edgeR software. CPA affected genes are involved in several biological functions, including stress response and immune-related pathways, oxi-reduction and apoptotic processes, and cuticle and vitelline membrane formation. In particular, this is the first report of CPA-induced mitochondrial dysfunction caused by the downregulation of genes involved with mitochondria constituents. CPA treatment also changed the transcription pattern of transposable elements (TEs) from the gypsy and copia superfamilies. The results presented here provided evidence of CPA mitochondrial toxicity mechanisms and that CPA can modify TEs transcription in Drosophila flies.

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The somatic mobilization of transposable element mariner-Mos1 during the Drosophila lifespan and its biological consequences

Cited by 10 publications

References 31 publications

Somatic Mobilization: High Somatic Insertion Rate of mariner Transposable Element in Drosophila simulans

Somatic Mobilization: High Somatic Insertion Rate of mariner Transposable Element in Drosophila simulans

Roles of Transposable Elements in the Different Layers of Gene Expression Regulation

Cyclophosphamide in Drosophila promotes genes and transposable elements differential expression and mitochondrial dysfunction

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