Hydroxyapatite nanoparticle based fluorometric turn-on determination of dipicolinic acid, a biomarker of bacterial spores

Abstract

Hydroxyapatite nanoparticles (HAP-NPs) were rendered fluorescence by doping with Eu(III) ion. The resulting fluorescent NPs are shown to be viable probes for sensitive and selective determination of dipicolinic acid (DPA), a major constituent of bacterial spores as used in bioterrorism. It is found that the addition of DPA to solutions of such HAP-NPs result in an enhancement of fluorescence due to the coordination of DPA with the Eu(III) dopant. The assay allows DPA to be detected in the 0.1 to 40 μM concentration range and with a 77 nM detection limit. The assay was applied to the detection of spores of Bacillus subtilis. The attractive properties of the probe make it a promising candidate for used in rapid detection of pathogenic bacterial spores. Graphical abstract Fluorescent hydroxyapatite nanoparticles (HAP-NPs) are shown to be a viable probe for detection of dipicolinic acid, a major constituent of bacterial spores. The red asterisks represent the fluorescence intensity of the HAP-NPs.

Rare-Earth Eu3+/Gold Nanocluster Ensemble-Based Fluorescent Photoinduced Electron Transfer Sensor for Biomarker Dipicolinic Acid Detection

“…This method is comparable to or even more efficient than most of the reported fluorescent sensing strategies (Table S2). ,,,− Moreover, the proposed assay is very facile and convenient without the requirements of probe modification, sophisticated instrument, cumbersome operation, time-consuming procedure, and toxic reagent.…”

In Situ Incorporation of Fluorophores in Zeolitic Imidazolate Framework-8 (ZIF-8) for Ratio-Dependent Detecting a Biomarker of Anthrax Spores

“…After that, 10 μL of dodecylamine solution (20 mM) was added to 90 μL of pretreated spores suspension and incubated with 200 μL of fluorescence probe immediately. Then fluorescence spectra were recorded every few minutes. , The changes of the luminescence intensity were also observed under the fluorescence inverted microscope every few minutes.…”

“…Then fluorescence spectra were recorded every few minutes. 34,35 The changes of the luminescence intensity were also observed under the fluorescence inverted microscope every few minutes.…”

Solid-Supported Amplification of Aggregation Emission: A Tetraphenylethylene–Cucurbit[6]uril@Hydroxyapatite-Based Supramolecular Sensing Assembly for the Detection of Spermine and Spermidine in Human Urine and Blood

“…Hydroxyapatite (HAp), with the chemical formula of Ca 10 (PO 4 ) 6 (OH) 2 , has received considerable attention as a biomaterial due to its close resemblance to the natural mineral apatite, which comprises the inorganic components of bones and teeth. − HAp nanoparticles (HAp NPs) possess good biocompatibility, low cytotoxicity, and good physical and chemical stability. Therefore, HAp NPs have found wide applications in medical fields like drug delivery, tissue engineering, bone repair, etc. − Recently, as good adsorbents, they have been used as attractive host materials of fluorescent nanocomposites for sensing applications as well. − …”