Mitochondrial ROS activate interleukin‑1β expression in allergic rhinitis

Shi, Qiankun; Lei, Zhiwei; Gang, Cheng; Li, Dehai; Wang, Qian; Luo, Simin; Yang, Hengwen; Jia, Haiying

doi:10.3892/ol.2018.8984

Cited by 26 publications

(35 citation statements)

References 56 publications

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“…This conclusion was determined by the application of SM7368, a specific NF-κB pathway inhibitor. mtROS are the main activators of the NLRP3 inflammasome; 52,53 thus, it is important to know whether IL-1β regulates mtROS production. According to our MitoSOX Red staining and western blotting results, IL-1β treatment reduced SOD2 expression and induced mtROS production in NP cells.…”

Section: Discussionmentioning

confidence: 99%

“…As previously described, mtROS production plays an important role in NLRP3 inflammasome activation. 52,53 Therefore, we examined whether mtROS production was involved in NLRP3 inflammasome activation in IL-1β-treated NP cells. We first detected that SOD2 expression was significantly decreased in the IL-1β treatment group compared with that of the control group, as measured by western blot and RT-qPCR analyses ( Fig.…”

Section: Il-1β Induces Nlrp3 Inflammasome Priming and Activation In Vmentioning

confidence: 99%

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Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop

et al. 2020

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The inflammatory response is induced by the overexpression of inflammatory cytokines, mainly interleukin (IL)-1β, and is one of the main causes of intervertebral disc degeneration (IVDD). NLR pyrin domain containing 3 (NLRP3) inflammasome activation is an important source of IL-1β. As an anti-inflammatory neuroendocrine hormone, melatonin plays various roles in different pathophysiological conditions. However, its roles in IVDD are still not well understood and require more examination. First, we demonstrated that melatonin delayed the progression of IVDD and relieved IVDD-related low back pain in a rat needle puncture IVDD model; moreover, NLRP3 inflammasome activation (NLRP3, p20, and IL-1β levels) was significantly upregulated in severely degenerated human discs and a rat IVDD model. Subsequently, an IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop was found in nucleus pulposus (NP) cells that were treated with IL-1β. In these cells, expression of NLRP3 and p20 was significantly increased, NF-κB signaling was involved in this regulation, and mitochondrial reactive oxygen species (mtROS) production increased. Furthermore, we found that melatonin disrupted the IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop in vitro and in vivo. Melatonin treatment decreased NLRP3, p20, and IL-1β levels by inhibiting NF-κB signaling and downregulating mtROS production. Finally, we showed that melatonin mediated the disruption of the positive feedback loop of IL-1β in vivo. In this study, we showed for the first time that IL-1β promotes its own expression by upregulating NLRP3 inflammasome activation. Furthermore, melatonin disrupts the IL-1β positive feedback loop and may be a potential therapeutic agent for IVDD.

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Section: Discussionmentioning

confidence: 99%

Section: Il-1β Induces Nlrp3 Inflammasome Priming and Activation In Vmentioning

confidence: 99%

Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop

et al. 2020

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“…1e), indicating that IL-18 is likely to play a role in sAR. Moreover, significant correlations between fIL-10 Elevated levels of IL-1β and TNF-α in plasma of patients with sAR Since monocytes and macrophages are involved in the pathogenesis of AR possibly by overproducing IL-1β and TNF-α [24][25][26][27], we examined levels of IL-1β and TNF-α in plasma of patients with pAR, sAR and HC by using human bio-plex panel kit. As shown in Fig.…”

Section: Elevated Level Of Il-18 In Plasma Of Patients With Par and Sarmentioning

confidence: 99%

Altered expression of IL-18, IL-18 binding protein and IL-18 receptor in blood monocytes of patients with allergic rhinitis

Wang

et al. 2020

Preprint

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Background: Interleukin (IL)-18 is emerging as an attractive participant in allergic rhinitis (AR). However, correlation of IL-18 with IL-18 binding protein (BP) in plasma, and expression of IL-18, IL-18BP and IL-18 receptor (R) in AR blood monocytes remains obscure. Methods: We investigated IL-18, IL-18BP and IL-18R expression in monocytes by using flow cytometric analysis, murine AR model, and quantitative realtime PCR in the present study. Results: It was found that plasma IL-18 and IL-1β in AR patients was higher than those in healthy control subjects. Free (f)IL-18 had a high correlation with IL-18BP, IL-1β and TNF-α in AR plasma. Proportion of IL-18 + monocytes was increased, whereas IL-18BP + monocytes were decreased in blood of patients with AR. It was found that Platanus pollen allergen extract provoked the elevated expression of IL-18 and IL-18R in AR blood monocytes. Dermatophagoides pteronyssinus , Artemisia sieversiana wild and Platanus pollen allergen extracts enhanced IL-18R protein and mRNA expression in primary monocytes from AR patients. Moreover, numbers of macrophages and IL-18R + macrophages in nasal lavage fluid (NLF) were increased, and levels of IL-18 in both plasma and NLF were elevated in AR mice. Conclusions: IL-18 is likely to participate in the development of AR as a causative factor, therefore it could be a therapeutic target for AR.

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“…Since monocytes and macrophages are involved in the pathogenesis of AR possibly by overproducing IL-1β and TNF-α [24][25][26][27], we examined levels of IL-1β and TNF-α in plasma of patients with pAR, sAR and HC by using human bio-plex panel kit. As shown in Fig.…”

Section: Elevated Levels Of Il-1β and Tnf-α In Plasma Of Patients Witmentioning

confidence: 99%

Altered expression of IL-18, IL-18 binding protein and IL-18 receptor in blood monocytes of patients with allergic rhinitis

Wang

et al. 2020

Preprint

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Background: Interleukin (IL)-18 is emerging as an attractive participant in allergic rhinitis (AR). However, correlation of IL-18 with IL-18 binding protein (BP) in plasma, and expression of IL-18, IL-18BP and IL-18 receptor (R) in AR blood monocytes remain obscure.Methods: We investigated IL-18, IL-18BP and IL-18R expression in monocytes by using flow cytometric analysis, murine AR model, and quantitative real-time PCR in the present study.Results: It was found that plasma IL-18 and IL-1β in AR patients was higher than those in healthy control subjects. Free (f)IL-18 had a high correlation with IL-18BP, IL-1β and TNF-α in AR plasma. Proportion of IL-18 + monocytes was increased, whereas IL-18BP + monocytes were decreased in blood of patients with AR. It was found that Platanus pollen allergen extract provoked the elevated expression of IL-18 and IL-18R in AR blood monocytes. Dermatophagoides pteronyssinus , Artemisia sieversiana wild and Platanus pollen allergen extracts enhanced IL-18R protein and mRNA expression in primary monocytes from AR patients. Moreover, numbers of macrophages and IL-18R + macrophages in nasal lavage fluid (NLF) were increased, and levels of IL-18 in both plasma and NLF were elevated in AR mice.Conclusions: IL-18 is likely to participate in the development of AR as a causative factor, therefore, it could be a therapeutic target for AR.

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Mitochondrial ROS activate interleukin‑1β expression in allergic rhinitis

Cited by 26 publications

References 56 publications

Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop

Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop

Altered expression of IL-18, IL-18 binding protein and IL-18 receptor in blood monocytes of patients with allergic rhinitis

Altered expression of IL-18, IL-18 binding protein and IL-18 receptor in blood monocytes of patients with allergic rhinitis

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