Effect of Astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development

Weng, Xiaogang; Cai, Mingming; Zhang, Yuting; Liu, Yan; Gao, Zheng-ling; Song, Jian; Z, Liu

doi:10.1016/j.theriogenology.2018.07.030

Cited by 21 publications

(9 citation statements)

References 25 publications

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“…After thawing, sperm quality, in terms of motility, viability, acrosome integrity, mitochondrial functionality and lipid peroxidation was assessed via flow cytometry. The addition of different doses of SSP to thawed sperm did not induce any significant effects on sperm motility and viability compared to control as already reported by other Authors who worked in the same experimental conditions but used different natural antioxidant components like epigallo-catechin-3-gallate (Gadani et al, 2017) and Astragalus polysaccharide (Weng et al, 2018), while total and progressive motility were negatively affected by resveratrol (Bucci et al, 2018). In our study, acrosome integrity, mitochondrial activity and lipid peroxidation were not influenced by SSP addition indicating that this natural extract has no effect on these parameters at the concentrations studied.…”

Section: Discussionsupporting

confidence: 73%

Improvement of in vitro fertilization by a tannin rich vegetal extract addition to frozen thawed boar sperm

et al. 2020

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Boar spermatozoa are very susceptible to cryopreservation injuries and, for this reason, pig remains one of the few species in which fresh semen is still preferred to thawed one for routine artificial insemination (AI). The present work evaluated the effect of supplementing boar sperm thawing medium with Silvafeed SP (SSP), a mixture of Chestnut and Quebracho wood extracts (60/40 w/w) rich in polyphenols (92.4% tannin content) on in vitro fertilization (IVF) and on the following sperm parameters: sperm motility (assessed by CASA), viability, acrosome integrity, mitochondrial function and lipid peroxidation (assessed by flow cytometry) and capacitation status (immunolocalization of tyrosine phosphorylated proteins). Thawed spermatozoa were incubated 1 h at 37°C in BTS without (CTR) or with (5, 10, 20 µg/mL) SSP. After incubation sperm suspension was divided in three aliquots: one was used for IVF trials, one for sperm analysis, and the last one was capacitated for 1 h at 39°C 5% CO2 in IVF medium. Sperm motility parameters, viability, acrosome integrity, mitochondrial functionality, lipid peroxidation and tyrosine phosphorylated protein immunolocalization, used as capacitation parameter, were not influenced by SSP. However, oocytes inseminated with thawed spermatozoa pretreated with all the different SSP concentrations presented a significant (P < 0.01) increase in penetration rate compared to CTR. In addition, 5 µg/mL SSP exerted a positive effect (P<0.05) on the total efficiency of fertilization. These results encourage the use of SSP in the thawing medium since post-thawing fertility is a limit for the largescale use of boar frozen semen.

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Section: Discussionsupporting

confidence: 73%

Improvement of in vitro fertilization by a tannin rich vegetal extract addition to frozen thawed boar sperm

et al. 2020

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“…The thawing extenders have been barely studied, but light modifications may substantially improve post-thawing sperm quality [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the thawing extender, it should be mentioned that porcine species are practically the only species that use it, with BTS being the most common, although other extenders have been tested. Supplementation of the thawing extender with compounds such as seminal plasma [ 12 , 13 ], cyclodextrins loaded with cholesterol (CLC) [ 14 ], antioxidants [ 15 , 16 , 17 ], ion chelators [ 18 ] or the modification of its pH [ 19 ] have shown substantial improvements in the sperm membrane integrity and also in the post-thaw movement quality.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Thawing Protocol for Iberian Boar Sperm

Tomás-Almenar¹,

Mercado²

2022

Animals

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Thawing protocols have been barely studied, and their modifications may lead to a substantial improvement in post-thawing sperm quality, which could be of great relevance to existing sperm banks, such as those for Iberian pig breeds with varieties in danger of extinction. For that, the study aimed to evaluate different thawing rates and to evaluate modifications in the composition of the thawing extender (basic pH to 8–8.2, incorporation of cyclodextrins loaded with cholesterol [CLC] and the incorporation of ion chelators [EDTA and EGTA]). After thawing, overall sperm motility and kinematic parameters, acrosome status and sperm membrane integrity were evaluated. The most optimal results were obtained with the thawing rate reaching 70 °C for 8 s with the inclusion of 12.5 mg of CLC/500 × 106 spermatozoa in the thawing extender, which showed an improvement compared to the control at 70 °C. In conclusion, to adapt the thawing conditions may be relevant, especially for endangered species or breeds such as some varieties of Iberian pig, since this process could also be used in samples cryopreserved in gene banks.

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“…Recent studies have found that APS could reduce the oxidative damage of cardiomyocytes caused by free radicals and lipid peroxides as well as reduce membrane damage. Furthermore, APSs have recently been found to have antibacterial and antiviral properties and can be used as immune enhancers [ 16 ]. Due to the various properties of APS, it can be used for the cryopreservation of pig semen, and studies have shown that it can improve semen vitality and antioxidant levels [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Wang

Yuan

et al. 2021

Animals

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The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis (CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels (p < 0.05), while reducing the ROS and MDA levels (p < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen.

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Effect of Astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development

Cited by 21 publications

References 25 publications

Improvement of in vitro fertilization by a tannin rich vegetal extract addition to frozen thawed boar sperm

Improvement of in vitro fertilization by a tannin rich vegetal extract addition to frozen thawed boar sperm

Optimization of the Thawing Protocol for Iberian Boar Sperm

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

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