PLGA film/Titanium nanotubues as a sustained growth factor releasing system for dental implants

Abstract

Ti-based implants sometimes fail to integrate with surrounding bone tissue due to insufficiency of new bone formation and surface bonding. To overcome this problem, this research focused on establishing a sustained bone growth factor delivery system by applying anodized TiO2 nanotube arrays and PLGA film on the titanium implant surface. TiO2 nanotube arrays were made by anodic oxidation method, and were then filled with rhBMP2 by vacuum freeze-drying. Next, PLGA was deposition on the surface of this material. The designed system was characterized, pharmacokinetic release rate of rhBMP2 was determined. Adhesion, proliferation, and differentiation activity of osteoblasts cultured on the new surfaces and traditional titanium surfaced were compared. SEM showed that a surface of TiO2 nanotube arrays were successfully generated. PLGA membranes of 50 nm, 250 nm, 800 nm thickness were successfully deposited on the surfaces of TiO2 nanotube layers by using 1%, 3%, 10% PLGA solutions. PLGA film of 250 nm thickness showed ideally controlled release of rhBMP2, lasting for 4 weeks. Furthermore, 250 nm thickness PLGA film improved osteoblast adhesion, proliferation, and levels of alkaline phosphatase. In conclusion, the PLGA film / TiO2 nanotube growth factor delivery system can effectively sustain the release of rhBMP-2, and promote proliferation and differentiation of MC3T3-E1 osteoblasts.

Safety and efficacy of tacrolimus-coated silicone plates as an alternative to mitomycin C in a rabbit model of conjunctival fibrosis

“…Recently, the TiO 2 nano-coating drug delivery system was successfully used as a sustained growth factor-releasing system for dental implants [44]. However, to the best of our knowledge, this is the first study to investigate the application of the TiO 2 nano-coating drug delivery system on a silicon plate for intraocular use.…”

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

“…For MTF coated with hrBMP‐2, with or without gentamicin, the differentiation was more than double the differentiation on bare MTFs. This result was expected as the effectiveness of hrBMP‐2 in promoting preosteoblast differentiation has been demonstrated in a large number of works …”

<p>Enhanced antibacterial properties of orthopedic implants by titanium nanotube surface modification: a review of current techniques</p>

“…Drug molecules need to go through the coatings to reach the surrounding environment, and the coating thickness plays a key role in this process 70,71,79. Wang et al71 used poly(lactic-co-glycolic acid) (PLGA) to cover drug-loaded TNTs, and the duration of release lengthened with the increasing thickness of the coating layers.…”

“…However, excessive thickness may excessively retard the release of the drug. Sun et al70 prepared drug-loaded TNTs coated with PLGA (thickness: 50 nm, 250 nm, and 800 nm). The results showed that the 250-nm PLGA TNT yielded the best release kinetics.…”