Zwitterionic Hydrogels Based on a Degradable Disulfide Carboxybetaine Cross-Linker

Jain, Priyesh; Hung, Hsiang‐Chieh; Li, Bowen; Ma, Jinrong; Dong, Dianyu; Lin, Xiaojie; Sinclair, Andrew; Zhang, Peng; O’Kelly, Mary Beth; Niu, Liqian; Jiang, Shaoyi

doi:10.1021/acs.langmuir.8b02100

Cited by 32 publications

(24 citation statements)

References 24 publications

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“…Recently, CB-based polymers show excellent hydration-induced nonfouling capability for various applications, making them very attractive for blood-contacting surface use. 20–24 Furthermore, CB groups have the unique capability to covalently immobilize biomolecules, such as proteins, enzymes and oligonucleartide, to their carboxyl groups. 25–27…”

Section: Introductionmentioning

confidence: 99%

Ultralow Fouling and Functionalizable Surface Chemistry Based on Zwitterionic Carboxybetaine Random Copolymers

Lin

Jain

et al. 2018

Langmuir

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Here, we report a simple, yet effective surface modification approach to impart hydrophobic surfaces with super-hydrophilicity using ultra-low fouling/functionalizable carboxybetaine (CB) copolymers via a dip-coating technique. A new series of CB random copolymers with varying amphiphilicities were synthesized and coated on hydrophobic polypropylene (PP) and polystyrene (PS) surfaces. Nonfouling capability of each coating was screened by enzyme-linked immunosorbent assay (ELISA), and further comprehensively assessed against 100% human serum by Micro BCA protein assay kit. The random copolymer containing ~30 mol% of CB unit showed superhydrophilicity with the highest air contact angle of more than 165° in DI water and the best nonfouling capability against 100% human blood serum. Surfaces of a 96-well plate coated with the optimal CB random copolymer showed significantly better nonfouling capability than those of a commercial 96-well plate with an ultra-low attachment surface. Adhesion of mouse embryonic fibroblast cells (NIH3T3) was completely inhibited on surfaces coated with CB random copolymers. Furthermore, the optimal nonfouling CB copolymer surface was functionalized with an antigen via covalent bonding where its specific interactions with its antibody were verified. Thus, this CB random copolymer is capable of imparting both ultra-low fouling and functionalizable capabilities to hydrophobic surfaces for blood-contacting devices.

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Section: Introductionmentioning

confidence: 99%

Ultralow Fouling and Functionalizable Surface Chemistry Based on Zwitterionic Carboxybetaine Random Copolymers

Lin

Jain

et al. 2018

Langmuir

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“…This versatile approach for encapsulation of biomolecules makes zwitterionic photoresists attractive for a wide range of biomedical applications for 3D-printed microrobotics, such as targeted drug/gene delivery, imaging, biosensing, enzyme therapy, etc. Future work will explore the development of advanced zwitterionic photoresist formulations to control the release of specific entrapped molecules in microrobots, including biodegradable zwitterionic crosslinkers [48] and active polymer networks. [34] 3D printing of zwitterionic microrobots allows not only for functionalization inside the polymer network but also for surface functionalization of the microstructures.…”

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confidence: 99%

Zwitterionic 3D‐Printed Non‐Immunogenic Stealth Microrobots

et al. 2020

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acoustic, [9,10] photo-, [11,12] thermal, [13,14] and chemical [14-17] actuation, for diverse medical applications, [1,2] such as targeted drug delivery, minimally invasive surgery, and remote sensing. However, many scientific challenges lie ahead before such untethered microrobots are ready for clinical use, such as biocompatibility, biodegradation, navigation in complex biofluids, or penetration of biological barriers. [18] Among them, robot interaction with the immune system is a major concern that hurdles their medical operation for long durations. The immune system is prepared to neutralize foreign organisms or materials as a natural protective mechanism, and microrobots are not an exception. When a microrobot enters the body (e.g., bloodstream), it would be rapidly coated with proteins via non-specific adsorption. [19-22] Macrophages, a type of immune cells that are on the lookout for pathogens, recognize these protein-coated materials as foreign threats and become activated, [19] leading to microrobot phagocytosis [23-25] (clearing them and disabling their functions) and eliciting an immune response. Therefore, the activation of macrophages is a major obstacle for developing functional medical microrobots that can operate in vivo for prolonged time. In order to surpass this first innate defense mechanism, we aim to prevent non-specific protein adsorption on the microrobot surface and avoid their detection by macrophages, Microrobots offer transformative solutions for non-invasive medical interventions due to their small size and untethered operation inside the human body. However, they must face the immune system as a natural protection mechanism against foreign threats. Here, non-immunogenic stealth zwitterionic microrobots that avoid recognition from immune cells are introduced. Fully zwitterionic photoresists are developed for two-photon polymerization 3D microprinting of hydrogel microrobots with ample functionalization: tunable mechanical properties, anti-biofouling and non-immunogenic properties, functionalization for magnetic actuation, encapsulation of biomolecules, and surface functionalization for drug delivery. Stealth microrobots avoid detection by macrophage cells of the innate immune system after exhaustive inspection (>90 hours), which has not been achieved in any microrobotic platform to date. These versatile zwitterionic materials eliminate a major roadblock in the development of biocompatible microrobots, and will serve as a toolbox of non-immunogenic materials for medical microrobot and other device technologies for bioengineering and biomedical applications.

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“…In a study done in 2019, Jain exploited the low fouling characteristic of polycarboxybetaine (PCB) polymers along with carboxybetaine disulfide cross-linker (CBX-SS) that facilitates degradation. The cross-linked PCB/CBX demonstrated excellent non-fouling properties and degradability, making it a promising material for future tissue engineering and drug delivery [54].…”

Section: Zwitterionic Polymersmentioning

confidence: 99%

Polymeric Scaffolds for Dental, Oral, and Craniofacial Regenerative Medicine

Munguia-Lopez

Cho

et al. 2021

Molecules

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Dental, oral, and craniofacial (DOC) regenerative medicine aims to repair or regenerate DOC tissues including teeth, dental pulp, periodontal tissues, salivary gland, temporomandibular joint (TMJ), hard (bone, cartilage), and soft (muscle, nerve, skin) tissues of the craniofacial complex. Polymeric materials have a broad range of applications in biomedical engineering and regenerative medicine functioning as tissue engineering scaffolds, carriers for cell-based therapies, and biomedical devices for delivery of drugs and biologics. The focus of this review is to discuss the properties and clinical indications of polymeric scaffold materials and extracellular matrix technologies for DOC regenerative medicine. More specifically, this review outlines the key properties, advantages and drawbacks of natural polymers including alginate, cellulose, chitosan, silk, collagen, gelatin, fibrin, laminin, decellularized extracellular matrix, and hyaluronic acid, as well as synthetic polymers including polylactic acid (PLA), polyglycolic acid (PGA), polycaprolactone (PCL), poly (ethylene glycol) (PEG), and Zwitterionic polymers. This review highlights key clinical applications of polymeric scaffolding materials to repair and/or regenerate various DOC tissues. Particularly, polymeric materials used in clinical procedures are discussed including alveolar ridge preservation, vertical and horizontal ridge augmentation, maxillary sinus augmentation, TMJ reconstruction, periodontal regeneration, periodontal/peri-implant plastic surgery, regenerative endodontics. In addition, polymeric scaffolds application in whole tooth and salivary gland regeneration are discussed.

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Zwitterionic Hydrogels Based on a Degradable Disulfide Carboxybetaine Cross-Linker

Cited by 32 publications

References 24 publications

Ultralow Fouling and Functionalizable Surface Chemistry Based on Zwitterionic Carboxybetaine Random Copolymers

Ultralow Fouling and Functionalizable Surface Chemistry Based on Zwitterionic Carboxybetaine Random Copolymers

Zwitterionic 3D‐Printed Non‐Immunogenic Stealth Microrobots

Polymeric Scaffolds for Dental, Oral, and Craniofacial Regenerative Medicine

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