Aquilariae Lignum extract attenuates glutamate-induced neuroexcitotoxicity in HT22 hippocampal cells

Lee, Jin-Seok; Kim, Wonyong; Jeon, Yoo-Jin; Lee, Sam-Keun; Son, Chang‐Gue

doi:10.1016/j.biopha.2018.07.032

Cited by 18 publications

(12 citation statements)

References 39 publications

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“…Interestingly, 4,6′-anhydrooxysporidinone was more effective than NAC, which is a popular positive control as an antioxidant in the study on glutamate-induced cell death for its ability to minimize oxidative stress [19,20]. This was confirmed by evaluating the reduced intracellular ROS, accumulation of intracellular ROS, increases in superoxide anion production, Ca 2+ , depolarization of mitochondrial membrane potential, and apoptotic cell death.…”

Section: Discussionmentioning

confidence: 87%

Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line

et al. 2020

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Excessive glutamate damages neuronal cells via the accumulation of intracellular reactive oxygen species (ROS), calcium ion (Ca2+) influx, depolarization of mitochondrial membrane potential, and apoptosis, which may result in the development of chronic neurodegenerative diseases. In this study, we evaluated the effects of 4,6′-anhydrooxysporidinone isolated from endophytic fungus Fusarium lateritium SSF2 on glutamate-induced cytotoxicity, accumulation of intracellular ROS, increases in superoxide anion production, Ca2+, depolarization of mitochondrial membrane potential, and apoptotic cell death in hippocampal HT22 cells. 2′,7′-Dichlorofluorescin diacetate (H2DCFDA) staining was used to determine the intracellular reactive oxygen species concentration and dihydroethidine (DHE) staining was used to determine the superoxide radical. Expression of the nuclear factor-erythroid-2–related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was analyzed by Western blot. Fluo-4 staining was used to determine the intracellular Ca2+ levels. In order to explore mitochondrial membrane potential, tetramethylrhodamine methyl ester (TMRM) staining was used. Apoptotic cell death was evaluated using Annexin-V/propidium iodide (PI) staining and TUNEL staining. Expression of the cytochrome c release and cleaved caspase-9, -3 was analyzed by Western blot. Here, we were able to isolate 4,6′-anhydrooxysporidinone from endophytic fungus, Fusarium lateritium SSF2, which was shown to protect HT22 cells from glutamate-induced cytotoxicity, accumulation of intracellular ROS, increases in superoxide anion production, Ca2+, and depolarization of mitochondrial membrane potential. In addition, 4,6′-anhydrooxysporidinone enhanced the expressions of Nrf2 and HO-1. It also inhibited the apoptotic cell death through the inhibition of cytochrome c release and cleaved caspase-9, -3 in glutamate-treated HT22 cells. Therefore, our results provide ample evidence of the neuroprotective properties of 4,6′-anhydrooxysporidinone.

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Section: Discussionmentioning

confidence: 87%

Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line

et al. 2020

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“…Under neuroinflammation, excessive release of inflammatory cytokines could alter the vascular remodeling of the blood–brain barrier (BBB), which eventually leads to neuronal death [ 19 , 20 ]. Our previous studies showed that ethanol extract of Aquilariae Lignum attenuates oxidative and inflammatory alterations in the mouse hippocampus and prevents hippocampal excitotoxicity [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported that Aquilariae Lignum has the potential to inhibit microglial overactivation in the mouse hippocampus [ 15 ]. In addition, we found neuroprotective effects of Aquilariae Lignum against glutamate-induced excitotoxicity in HT22 cells [ 16 ]. The above findings suggest the involvement of microglia-derived neuroinflammation as a target mechanism of Aquilariae Lignum.…”

Section: Introductionmentioning

confidence: 99%

Aquilariae Lignum Methylene Chloride Fraction Attenuates IL-1β-Driven Neuroinflammation in BV2 Microglial Cells

Lee

Jeon

Kang

et al. 2020

IJMS

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Microglial hyperactivation and neuroinflammation are known to induce neuronal death, which is one of the main causes of neurodegenerative disorders. We previously found that Aquilariae Lignum extract attenuated both neuronal excitotoxicity and neuroinflammation in vivo and in vitro. For further analysis, we extracted the methylene chloride fraction of Aquilariae Lignum to determine the bioactive compounds. In this study, we investigated the anti-neuroinflammatory effects and underlying mechanisms of the Aquilariae Lignum fraction (ALF) using lipopolysaccharide (LPS)-stimulated BV2 microglial cells. BV2 cells were pretreated with ALF (0.5, 1, and 2.5 μg/mL) before treatment with LPS (1 μg/mL). Pretreatment with ALF significantly attenuated the LPS-induced overproductions of nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), and interleukin (IL)-1β. These anti-inflammatory effects were supported by ALF-mediated modulation of the nuclear factor-kappa B (NF-κB) pathway. Furthermore, ALF exerted strong anti-inflammasome effects, as shown by IL-1β-specific inhibitory activity, but not activity against tumor necrosis factor (TNF)-α, along with inhibition of caspase-1 activity and NACHT, LRR, and PYD domain-containing protein 3 (NLRP3)-related molecules. These results indicate the potent anti-neuroinflammatory activity of ALF and that its underlying mechanism may involve the regulation of NLRP3 inflammasome-derived neuroinflammation in microglial cells.

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“…Most organic compounds can be dissolved in ethanol except protein, phlegm, pectin, starch and polysaccharide (Xu et al, 2011). There are three studies that use ethanol as the extraction solvent which are Study 4 (Liao et al, 2016), Study 5 (Lee et al, 2018) and Study 6 (Dong et al, 2019). All studies have performed the isolation for targeted compounds from the ethanol extract of agarwood to test the pharmacological effects of certain diseases in vitro.…”

Section: Ethanolmentioning

confidence: 99%

“…The compounds were assessed for α-glucosidase inhibitory activity, AchE inhibitory activity and cytotoxic activity against BEL-7402, K562, and SGC-7901 human cancer cell lines. The study also revealed that 6,7-dimethoxy-2- Study 5 was done by investigating the pharmacological effect of Aquilaria lignum against glutamate-induced neuroexcitotoxicity and its underlying mechanisms using HT22 cells, a mouse hippocampal neuron cell line (Lee et al, 2018). A. lignum resinous heartwood was extracted using 30% ethanol for 72 h. Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was conducted on A. lignum extract to determine its chemical composition (Lee et al, 2017).…”

Section: Ethanolmentioning

confidence: 99%

Solvent used in extraction process of agarwood: a systematic review

Zakaria¹,

Talip²,

Kahar³

et al. 2020

Food Res.

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The method of extraction of Agarwood is usually dependent on the purpose of the extract. This systematic review aims to look at the number of studies that use different types of solvents in the process of extraction. There are 3 types of solvents commonly used in the extraction of agarwood essential oils namely methanol, ethanol and water. Each solvent produces different extracts in terms of quantity and quality of the final product. However, there is controversy over the use of solvents as it may cause a cytotoxic effect on the user. Another common type of solvent is water. Although water is a cheap solvent and relatively safe, aqueous extracts have more impurities that make isolating the desired compound difficult. After the extraction process, the crude extract was fractionated into the desired compounds and this technique is widely applied, especially in the whole process of extraction of the agarwood. In conclusion, extraction solvents have certain advantages and disadvantages, and this is one of the reasons why this extraction method depends on the purpose of using the final product such as agarwood essential oil.

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Aquilariae Lignum extract attenuates glutamate-induced neuroexcitotoxicity in HT22 hippocampal cells

Cited by 18 publications

References 39 publications

Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line

Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line

Aquilariae Lignum Methylene Chloride Fraction Attenuates IL-1β-Driven Neuroinflammation in BV2 Microglial Cells

Solvent used in extraction process of agarwood: a systematic review

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