A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

Bhosale, Rahul; Boudolf, Véronique; Cuevas, Fabiola; Lu, Ran; Eekhout, Thomas; Hu, Zhubing; Isterdael, Gert Van; Lambert, Georgina M.; Xu, Fan; Nowack, Moritz K.; Smith, Richard S.; Vercauteren, Ilse; Rycke, Riet Maria De; Storme, Véronique; Beeckman, Tom; Larkin, John C.; Kremer, Anna; Höfte, Monica; Galbraith, David W.; Kumpf, Robert P.; Maere, Steven; Veylder, Lieven De

doi:10.1105/tpc.17.00983

Cited by 91 publications

(113 citation statements)

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“…Generally, under stressful conditions, plant growth is also regulated by the synthesis of several phytohormones, such as jasmonic acid, salicylic acid, auxins, gibberellins and cytokinins (growth promoters) [87,88], ethylene, and abscisic acid (growth retardants). It has been reported that soil salinity enhanced the abscisic acid level in Zea mays L. at the expense of auxins (IAA) [89].…”

Section: Salinity Tolerance Mechanisms Adopted By Crop Plantsmentioning

confidence: 99%

An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation

Kamran

Parveen

Ahmar

et al. 2019

IJMS

342

200

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Soil salinization is one of the major environmental stressors hampering the growth and yield of crops all over the world. A wide spectrum of physiological and biochemical alterations of plants are induced by salinity, which causes lowered water potential in the soil solution, ionic disequilibrium, specific ion effects, and a higher accumulation of reactive oxygen species (ROS). For many years, numerous investigations have been made into salinity stresses and attempts to minimize the losses of plant productivity, including the effects of phytohormones, osmoprotectants, antioxidants, polyamines, and trace elements. One of the protectants, selenium (Se), has been found to be effective in improving growth and inducing tolerance against excessive soil salinity. However, the in-depth mechanisms of Se-induced salinity tolerance are still unclear. This review refines the knowledge involved in Se-mediated improvements of plant growth when subjected to salinity and suggests future perspectives as well as several research limitations in this field.

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Section: Salinity Tolerance Mechanisms Adopted By Crop Plantsmentioning

confidence: 99%

An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation

Kamran

Parveen

Ahmar

et al. 2019

IJMS

342

200

View full text Add to dashboard Cite

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“…The DEGs of differential libraries were screened according to the method described previously [72][73]. Further analysis of reads derived from all libraries was carried out by NOISeq technique, which was reported by Cheng et al (2018) [33].…”

Section: Screening Of Differentially Expressed Genes (Degs)mentioning

confidence: 99%

Gene expression profiling reveals light regulation on adventitious root formation in lotus (Nelumbo nucifera Gaertn.)

Cheng¹,

yuyan²,

minrong³

et al. 2020

Preprint

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Background: Lotus is an aquatic horticultural crop, and widely cultivated in most regions of China.Lotus is often used as a kind of an important off-season vegetable with various nutrients. Principle root of lotus is degenerated, and the role of adventitious roots (ARs) is Irreplaceable for plant growth.We found that no ARs could be formed under darkness condition, and high light significantly promote the development of root primordium. Therefore, four libraries with three light intensities were constructed to monitor metabolism changes, especially in IAA and sugar metabolism.Results: We found that ARs formation was significantly affected by light, high light intensity accelerated ARs development. The change of metabolism during ARs formation under different light intensity was evaluated according to gene expression profiling by high-throughput tag-sequencing. It was shown that more than 2.2× 10 4 genes was obtained in each library, and the expression level of most genes were distributed between 1e-01 and 1e+03 (FPKF value). Among these identified genes, 1739, 1683 and 1462 genes were up-regulated, and 1533, 995 and 834 genes were down-regulated in D/CK, E/CK and F/CK libraries respectively. In addition, we also found that 1454 and 478 genes changed expression in D/CK and F/CK libraries when compared D/CK with F/CK libraries. In F/D libraries, the transcriptional level of most differentially expressed genes was between -5~5 fold, and twenty differentially expressed genes were involved in signal transduction pathway. Twenty-eight genes related with IAA response and thirty-five genes involved in sugar metabolism were found to change expression. It was elucidated that IAA content was enhanced after seed germinated, even in darkness condition, which was responsible for ARs formation.Conclusion: The process of ARs formation was very complex, and regulated by multiple factors. The ARs formation was regulated by IAA, even in the dark, induction and developmental process could also be completed. In addition, the genes (36 genes) changed expression level in carbohydrate metabolism showed the third number, so sucrose metabolism was involved in the ARs development (expressed stage) according to genes expression and content change characteristic.

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“…The screening for differentially expressed genes (DEGs) was performed as described previously [58][59]. The resulting reads were used for the downstream analysis with NOISeq method as described by database.…”

Section: Screening For Differentially Expressed Gene and Small Mirnasmentioning

confidence: 99%

Comparative transcriptome analysis revealed the cooperative regulation of sucrose and IAA on adventitious root formation in lotus (Nelumbo nucifera Gaertn)

Cheng

Minrong

Liu

et al. 2019

Preprint

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BackgroundIn China, lotus is an important cultivated crop with multiple applications in ornaments, food, and environmental purification. Adventitious roots (ARs), a secondary root is necessary for the uptake of nutrition and water as the lotus principle root is underdeveloped. To investigate the regulatory pathway during ARs formation, we analyzed the expression of genes and miRNAs upon treatment with differential concentrations of sucrose, and a crosstalk between sucrose and IAA was also identified. ResultsWe observed that the lotus ARs formation was significantly affected by sucrose treatment. Notably, 20 mg/L sucrose promoted the ARs development, whereas 60 mg/L sucrose inhibited the formation of ARs. The expression of genes and miRNAs was evaluated by high-throughput tag-sequencing. We observed that the expression of 5438, 5184, and 5345 genes was enhanced in the GL20/CK0, GL60/CK0, and CK1/CK0 libraries, respectively. Further, the expression of 73, 78, and 71 miRNAs was upregulated in the ZT20/MCK0, ZT60/MCK0, and MCK1/MCK0 libraries, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that most of the differentially expressed genes and miRNAs in the GL20/GL60 and ZT20/ZT60 libraries were involved in signal transduction. A large number of these genes (29) and miRNAs (53) were associated with plant hormone metabolism. We observed an association between five miRNAs (miR160, miR156a-5p, miR397-5p_1, miR396a and miR167d) and nine genes (auxin response factor, protein brassinosteroid insensitive 1, laccase, and peroxidase 27) in the ZT20/ ZT60 libraries during ARs formation.Quantitative polymerase chain reaction (qPCR) was used to validate the high-throughput tagsequencing data. ConclusionsWe found that the expression of many critical genes involved in IAA synthesis and IAA transport was changed after treatment with various concentration of sucrose. Based on the change of these genes expression, IAA and sucrose content, we concluded that sucrose and IAA cooperatively regulated ARs formation. Sucrose affected ARs formation by improving IAA content at induction stage, and increased

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A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

Cited by 91 publications

References 100 publications

An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation

An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation

Gene expression profiling reveals light regulation on adventitious root formation in lotus (Nelumbo nucifera Gaertn.)

Comparative transcriptome analysis revealed the cooperative regulation of sucrose and IAA on adventitious root formation in lotus (Nelumbo nucifera Gaertn)

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