Therapeutic Intervention of Aloe Gel Containing Nano-Sized and Micron-Sized Silver Sulfadiazine Gel on Second-Degree Burn: A Comparative Study

Abstract

The current work focuses on the formulation development, optimization, and in vivo assessment of nano-sized silver sulfadiazine ( nSSD) and micron-sized silver sulfadiazine ( mSSD) topical gel composed of Aloe vera gel ( Aloe gel) and Carbopol 940 for the management of second-degree burn wound. The optimized concentration of gel-forming agent (Carbopol 940) was chosen based on best possible consistency and spreadability of the gel. The second-degree burn infliction was developed in the posterior region of rats followed by anesthesia. Afterward, the created wounds were further treated individually by both the gel formulation (1 application daily) for 14 days and observations were recorded. The nSSD gel showed better wound healing and a higher degree of tissue hyperplasia as compared with mSSD gel in rats. In vitro drug release study showed better drug release from nSSD gel (74.25 ± 3.331%) as compared with mSSD gel formulation (61.32 ± 2.112%) after 24 hours. The nSSD and mSSD topical gel-treated rats showed 95.63% and 78.75% wound healing after 14 days, while in the case of control group rats, 48.65% wound contraction was seen after 14 days. Furthermore, the histopathological study revealed that the nSSD gel was more efficient in controlling the wound infection and showed better wound healing as compared with mSSD gel formulation.

The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat

“…This result shows that CM and MEM-α+FFP 10% have better wound healing activity than SSD cream. This phenomenon might be explained because CM and placebo consist of growth factors and other proteins, which affect wound healing phases [ 3 , 4 ], while SSD is an antibacterial agent that can delay wound re-epithelization [ 24 ]. In vitro study shows that MSC-CM can fasten fibroblast and keratinocyte migration which was suspected because of growth factors, cytokines, and other proteins in MSC-CM [ 10 ].…”

A Systematic Review and Meta-Analysis Comparing Burn Healing Outcomes Between Silver Sulfadiazine and Aloe vera

“…Some key constituents include mannose-6-phosphate, salicylic acid, lignin, and saponin. Mannose-6-phosphate facilitates collagen synthesis and enhances the rate of wound contraction and macrophage function [15]. Wound treatment with Aloe also increases the expression of β1-, α6-, and β4-integrin and E-cadherin in human primary epidermal keratinocytes, contributing to cell migration and wound healing [16].…”