Long noncoding RNA H19, and not microRNA miR-326, is over-expressed and predicts survival in glioblastoma

Abstract

Glioblastoma (GB) represents the most common malignant brain tumor, which, despite extensive research, remains of poor prognosis. The focus of recent studies of GB pathogenesis has shifted to the study of the role of noncoding RNAs (ncRNAs). In this study, we examined the expression levels of the microRNA miR-326 and the long ncRNA H19 (on which a miR-326 putative binding site was found by in silico analysis) in brain tumor tissue from GB patients as compared to cancer-free brain tissue. Relative expression levels of miR-326 were not found to be significantly altered in GB patients. On the contrary, H19 was consistently over-expressed in all GB patients (p < 0.001), and was correlated with poorer overall survival (OS) and progression-free survival (PFS) (p = 0.026 and p = 0.045, respectively). At a cutoff value of 5.27, H19 upregulation could predict OS in GB patients, with a 71.4% sensitivity and 59.6% specificity (p = 0.026). The current GB patients were clustered by the multivariate analysis into four groups based on miR-326 and H19 expression profiles, age at diagnosis, and PFS. Our data suggest a role for H19 in the pathogenesis of GB and could be a potential prognostic biomarker for GB.

Circular RNA hsa_circ_0061825 (circ‐TFF1) contributes to breast cancer progression through targeting miR‐326/TFF1 signalling

“…For instances, circPUM1 facilitates the malignance of lung adenocarcinoma via regulating miR-326 28 ; HOTAIR-miR-326-FUT6 axis promotes colorectal cancer progression by modulating fucosylation of CD44 through PI3K/AKT/mTOR pathway 29 ; hsa_circ_0003998 accelerates cell proliferation and invasion via absorbing miR-326 in nonsmall-cell lung cancer 30 ; and lncRNA H19, in contrast to miR-326, is overexpressed and predicts poor survival in glioblastoma. 31 Mechanism assays certified that circ-TFF1 promotes TFF1 expression by absorbing miR-326. Furthermore, rescue and in vivo experiments revealed that circ-TFF1 targets miR-326 to enhance TFF1 expression for the cellular activities of breast cancer.…”

Expression of microRNAs miR-21 and miR-326 associated with HIF-1α regulation in neurospheres of glioblastoma submitted to ionizing radiation treatment

“…It is speculated that other molecular agents interact with miR-326 to positively regulate glioblastoma and establish miR-326 as a feedback function [ 16 ].…”

“…H19 high expression was demonstrated in glioblastomas, and angiogenesis and an increase of invasiveness were confirmed. This contradicts the overexpression of miR-326 in gliomas, and it results in decreased cell proliferation and an apoptotic increase [ 16 ].…”

Long Non-Coding RNAs in Diagnosis, Treatment, Prognosis, and Progression of Glioma: A State-of-the-Art Review

“…Prognostic value : H19 overexpression in glioma tissue was associated with poor overall and progression-free survival and more advanced tumor stage ( 45 , 46 , 48 , 64 ). Moreover, serum levels of H19 showed a significant positive correlation with tumor grade ( 62 ).…”