Proteome Profiles of Head Kidney and Spleen of Rainbow Trout (<i>Oncorhynchus Mykiss</i>)

Kumar, Gokhlesh; Hummel, Karin Anna; Razzazi-Fazeli, Ebrahim; El-Matbouli, Mansour

doi:10.1002/pmic.201800101

Cited by 20 publications

(8 citation statements)

References 26 publications

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“…In fish, the spleen and kidney have important functions in implementing immune defence and the maintenance of a stable internal environment (Kumar et al, 2018). In the kidney, both chronic and acute intoxication of cadmium have been indicated to cause nephropathies.…”

Section: Discussionmentioning

confidence: 99%

“…Although cadmium levels were still higher than in the control group, this finding can be accepted as a protective effect of melatonin against cadmium accumulation in tissues. According to Chwełatiuk et al (2006), Hussein et al 2014 The accumulation of cadmium in the kidney and spleen as the organs of the haematopoietic system may disturb hematopoietic processes (Taheri et al, 2016;Kumar et al, 2018). Kondera and Witeska (2013) and Sun et al (2019) reported that cadmium reduced the hematopoietic potential in common carp (Cyprinus carpio L.) and yellow catfish (Pelteobagrus fulvidraco) in the head kidney and spleen, and caused histological lesions, antioxidative and inflammatory responses in both tissues.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Melatonin on Cadmium Accumulation and Haematological Parameters in Cadmium Intoxicated Prussian Carp (Carassius gibelio B.)

Drąg‐Kozak

Łuszczek‐Trojnar

Socha

et al. 2021

Annals of Animal Science

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The present study was performed to determine the effect of melatonin on cadmium accumulation and haematological parameters changes in females of Prussian carp (Carassius gibelio B.). The fish were exposed to a sublethal concentration of cadmium (0.4 and 4.0 mg/L) without or with melatonin implant (containing 18 mg melatonin hormone) for 1, 4, 7, 10 and 13 weeks. After the 7th week, Cd-exposed fish were divided into two groups. The first group of fish were subjected to depuration in clear water, while the second group remained exposed to the same concentrations of cadmium. After the 10th and 13th weeks of exposure the kidney, spleen, heart and blood were taken in order to determine the cadmium concentration and to analyse haematological parameters. The results of this study showed an increase in the concentration of cadmium in the tissues and blood in fish exposed to the highest dose (4.0 mg/L) of cadmium in water with a dependence on the exposure duration. Additionally, it was shown that melatonin administration reduced the cadmium concentration in the tissues and blood, and melatonin had a protective effect against cadmium accumulation. Moreover, the results showed a significant increase in blood parameters (RBC, Ht and Hb) in fish treated with the highest dose of cadmium. However, implantation of melatonin in cadmium-treated fish resulted in marked improvements in haematological parameters. Thus, melatonin can be a good implementation to alleviate, at least partially, the toxicity of cadmium.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Melatonin on Cadmium Accumulation and Haematological Parameters in Cadmium Intoxicated Prussian Carp (Carassius gibelio B.)

Drąg‐Kozak

Łuszczek‐Trojnar

Socha

et al. 2021

Annals of Animal Science

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“…In zebrafish, 3,024 proteins were reported [77], 939 in three-spined sticklebacks (Gasterosteus aculeatus) [78], and 717 in Atlantic cod (Gadus morhua) (identification based on a minimum of one peptide for all three species) [79]. Interestingly, regarding other tissues and biofluids of rainbow trout, 3,241 proteins were reported in head kidney (identification based on a minimum of two peptides), 2,542 in spleen (identification based on a minimum of two peptides) [80], 59 in ovarian fluid (identification based on a minimum of two unique peptides) [81] and 152 in seminal plasma [82]. Differences in the number of protein identifications can have both biological (fish tissues and fluids used) and technical origin (sample processing, fractionation strategy, MS instrument type, data acquisition parameters, data trimming and database identification version and parameters).…”

Section: Discussionmentioning

confidence: 99%

Plasma proteome profiling of freshwater and seawater life stages of rainbow trout (Oncorhynchus mykiss)

et al. 2020

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The sea-run phenotype of rainbow trout (Oncorhynchus mykiss), like other anadromous salmonids, present a juvenile stage fully adapted to life in freshwater known as parr. Development in freshwater is followed by the smolt stage, where preadaptations needed for seawater life are developed making fish ready to migrate to the ocean, after which event they become post-smolts. While these three life stages have been studied using a variety of approaches, proteomics has never been used for such purpose. The present study characterised the blood plasma proteome of parr, smolt and post-smolt rainbow trout using a gel electrophoresis liquid chromatography tandem mass spectrometry approach alone or in combination with low-abundant protein enrichment technology (combinatorial peptide ligand library). In total, 1,822 proteins were quantified, 17.95% of them being detected only in plasma post enrichment. Across all life stages, the most abundant proteins were ankyrin-2, DNA primase large subunit, actin, serum albumin, apolipoproteins, hemoglobin subunits, hemopexin-like proteins and complement C3. When comparing the different life stages, 17 proteins involved in mechanisms to cope with hyperosmotic stress and retinal changes, as well as the downregulation of nonessential processes in smolts, were significantly different between parr and smolt samples. On the other hand, 11 proteins related to increased growth in post-smolts, and also related to coping with hyperosmotic stress and to retinal changes, were significantly different between smolt and post-smolt samples. Overall, this study presents a series of proteins with the potential to complement current seawater-readiness assessment tests in rainbow trout, which can be measured non-lethally in an easily accessible biofluid. Furthermore, this study represents a first in-depth characterisation of the rainbow trout blood plasma proteome, having considered three life stages of the fish and used both fractionation alone or in combination with enrichment methods to increase protein detection. PLOS ONE | https://doi.org/10.1371/journal.pone.0227003 January 3, 2020 1 / 23 OPEN ACCESS Citation: Morro B, Doherty MK, Balseiro P, Handeland SO, MacKenzie S, Sveier H, et al. (2020) Plasma proteome profiling of freshwater and seawater life stages of rainbow trout (Oncorhynchus mykiss). PLoS ONE 15(1): e0227003. https://doi.org/10.

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“…Likewise, an intense expression of lep and lepr was observed in spleen and head kidney of C. punctata though the pattern varied from secondary to primary lymphoid organ. Recent studies in fishes have also shown marked difference between spleen and head kidney with respect to expression pattern of immune-related genes and their respective proteins in response to bacterial infection [34][35][36] . These differences have been attributed to differential role of primary and secondary lymphoid organs as teleostean spleen is proposed to be majorly involved in cellular responses while head kidney in humoral responses 34 .…”

Section: Discussionmentioning

confidence: 99%

Reproductive phase-dependent variation, sexually dimorphic expression and sex steroids-mediated transcriptional regulation of lep and lepr in lymphoid organs of Channa punctata

Bakshi

Rai

2020

Sci Rep

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The reproductive phase-dependent and sex-related differential expression of leptin (lep) and its receptor (lepr) in primary and secondary lymphoid organs of a highly nutritive economically important Channa punctata preempts the involvement of sex steroids in modulating intra-immuno-leptin system. This hypothesis was strengthened when plasma testosterone (T) and estradiol (E 2) levels in male and female fish of reproductively active spawning and quiescent phases were correlated with lep and lepr expression in their immune organs. Splenic lep and lepr showed a negative correlation with t in both male and female, while with e 2 there was a positive correlation in male and negative in female C. punctata. in head kidney, a contrasting correlation was observed as compared to spleen. to validate the implication of sex steroids in regulating leptin system in immune organs, in vivo and in vitro experiments were performed with DHt and e 2. Upon administration, lep and lepr expression in tissues of either sex was downregulated. in addition, in vitro results with either of the sex steroids exemplified their direct involvement. Overall, this study, for the first time, reports correlation between sex steroids and transcript expression of leptin system in immune organs of a seasonally breeding vertebrate. Leptin encoded by obese gene Lep also known as Ob is a 16 kDa non-glycosylated protein belonging to class I cytokine family and is primarily produced from adipose tissue in mammals 1. It is reported to act through long form of membrane-bound leptin receptor (Lepr) 2. Besides acting as a central link between feeding, adiposity and energy homeostasis 3 , leptin regulates several other physiological functions in mammals including reproduction 4 and immunity 5. The orthologs of Lep and Lepr have been identified in several teleosts 6-10 in which liver, and not the adipocytes, is reported to be the major leptin-expressing organ. Regarding physiological significance, hepatic lep is suggested to be majorly involved in orchestration of energy trade-off rather than regulating food intake in fishes 9,11-13. In addition to liver, expression of lep is shown in other tissues, including immune organs 6,10,11. Since immune defence varies with state of reproductive activity in seasonally breeding vertebrates 14,15 , an effort needs to be made to examine correlation between leptin, immunity and reproduction. In addition, sexual dimorphism is unveiled in levels of leptin in blood plasma 16,17 and adipose tissue 18,19 in mammals, being significantly higher in females than males. Interestingly, these sex-related differences in leptin protein and mRNA levels exist regardless of the amount of body fat 16. This led to hypothesize the involvement of sex steroids in modulating leptin expression 20. With regard to sexually dimorphic expression of leptin receptor, reports in mammals are limited and present contradictory results 21,22. In teleosts, studies on sex-related variation in plasma levels of leptin 23,24 or expression of lep and lepr have been me...

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Proteome Profiles of Head Kidney and Spleen of Rainbow Trout (Oncorhynchus Mykiss)

Cited by 20 publications

References 26 publications

Effects of Melatonin on Cadmium Accumulation and Haematological Parameters in Cadmium Intoxicated Prussian Carp (Carassius gibelio B.)

Effects of Melatonin on Cadmium Accumulation and Haematological Parameters in Cadmium Intoxicated Prussian Carp (Carassius gibelio B.)

Plasma proteome profiling of freshwater and seawater life stages of rainbow trout (Oncorhynchus mykiss)

Reproductive phase-dependent variation, sexually dimorphic expression and sex steroids-mediated transcriptional regulation of lep and lepr in lymphoid organs of Channa punctata

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