Respirometric reserve capacity of cumulus cell mitochondria correlates with oocyte maturity

Anderson, Sharon; Glassner, M.J.; Melnikov, Andrey; Friedman, Gary D.; Orynbayeva, Zulfiya

doi:10.1007/s10815-018-1271-9

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…This is important since it reveals that the mitochondrial mass (total respiratory capacity) does not appear to be affected by aging. A recent study, that used flow cytometry to evaluate mitochondrial mass and respiratory capacity of CCs, failed to detect significant differences between patients with less than 35 and more than 35 years of age (Anderson et al, 2018). Therefore, the molecular basis for the diminished energy metabolism cannot be envisaged at present.…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of the bioenergetic properties of human mural luteinized GCs and CCs presented have allowed the progress in the understanding of the metabolism of these two follicular cell types that are known to influence the maturation of the oocyte and, ultimately, IVF outcomes (Shufaro et al, 2012;Liu et al, 2017). These cells are believed to be potential biomarkers of oocyte quality (Dumesic et al, 2015;Anderson et al, 2018), and it has been proposed that alterations in their energy metabolism could lead to infertility (Shufaro et al, 2012;Hsu et al, 2014;Dong et al, 2016;Liu et al, 2017). The results evidence (i) the high glycolytic profile of these two cell types, (ii) that the respiratory capacity relies on the presence of follicular fluid in the experimental media, and (iii) that luteinized GCs from aged women present lower respiration and aerobic glycolysis which lead to a decrease in the cellular ATP levels.…”

Section: Discussionmentioning

confidence: 99%

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Reproductive senescence impairs the energy metabolism of human luteinized granulosa cells

Cecchino¹,

García-Velasco²,

Rial³

2021

Reproductive BioMedicine Online

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RESEARCH QUESTION: Female age is the single greatest factor influencing reproductive performance and granulosa cells are considered as potential biomarkers of oocyte quality. Is there an age-effect on the energy metabolism of human mural granulosa cells? DESIGN: Observational prospective cohort and experimental study including 127 women that underwent in vitro fertilization cycles. Women were allocated to two groups: a group of infertile patients aged over 38 years and a control group comprising oocyte donors aged less than 35 years.Individuals with pathologies that could impair fertility were excluded from both groups. Following oocyte retrieval, cumulus and granulosa cells were isolated and their bioenergetic properties (oxidative phosphorylation parameters, rate of aerobic glycolysis and adenine nucleotide levels) were analyzed and compared. RESULTS:Human mural luteinized granulosa and cumulus cells present high rates of aerobic glycolysis that cannot be increased further when mitochondrial ATP synthesis is inhibited. Addition of follicular fluid to the experimental media is necessary to reach the full respiratory capacity of the cells. Granulosa cells from aged women present lower mitochondrial respiration, although mitochondrial mass is not decreased, and lower aerobic glycolysis than those from young donors. The concurrent decrease in the two energy supply pathways leads to a decrease in the cellular ATP levels. CONCLUSIONS:Human mural luteinized granulosa cells exhibit a reduction in their energy metabolism as women age that is likely to influence female reproductive potential.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Reproductive senescence impairs the energy metabolism of human luteinized granulosa cells

Cecchino¹,

García-Velasco²,

Rial³

2021

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…The analysis of the bioenergetic properties of human mural GCs and CCs presented have allowed the progress in the understanding of the metabolism of these two follicular cell types that are known to influence the maturation of the oocyte and, ultimately, IVF outcomes [41][42]. These cells are believed to be potential biomarkers of oocyte quality [39,43], and it has been proposed that alterations in their energy metabolism could lead to infertility [27,41,42,44]. The results evidence (i) the high glycolytic profile of these two cell types and (ii) that the respiratory capacity relies on the presence of follicular fluid in the experimental media ( Figure 1 and Supplemental Figure 3).…”

Section: Discussionmentioning

confidence: 99%

“…This is important since it reveals that the mitochondrial mass (total respiratory capacity) does not appear to be affected by aging. A recent study, that used flow cytometry to evaluate mitochondrial mass and respiratory capacity of CCs, failed to detect significant differences between patients with less than 35 and more than 35 years of age [43]. Therefore, the molecular basis for the diminished energy metabolism cannot be envisaged at present.…”

Section: Discussionmentioning

confidence: 99%

Reproductive Senescence Impairs the Energy Metabolism of Human Granulosa Cells

Cecchino

Garcia-Velasco

Rial

2021

Preprint

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Female age is the single greatest factor influencing reproductive performance. It is widely known that mitochondrial dysfunction plays a key role in reproductive senescence. Ovarian bioenergetics includes a sophisticated metabolic synergism between oocytes and human mural granulosa cells (GCs), which is crucial for oocyte maturation during follicular growth. These cells are believed to be potential biomarkers of oocyte quality. It has been proposed that alterations in their energy metabolism could lead to infertility. We investigated if there is an age-related effect on the energy metabolism of human mural granulosa cells. We performed an observational prospective cohort and experimental study including 127 women that underwent in vitro fertilization cycles allocated to two groups: a control group comprising oocyte donors aged less than 35 years and a group of infertile women aged over 38 years. The bioenergetics of cumulus cells and purified mural GCs were determined from oxidative phosphorylation parameters, aerobic glycolysis and adenine nucleotide levels. We have found that human mural GCs and cumulus cells present a high glycolytic profile and that the follicular fluid is critical to sustain their energy metabolism. GCs from older women present lower mitochondrial respiration and glycolysis than those from young donors which is not accompanied by a lower respiratory capacity. The diminished energy metabolism leads to a decrease in the total cellular energy charge. We conclude that, as women age, mural granulosa cells exhibit a reduction in their energy metabolism that is likely to influence female reproductive potential.

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Re-denudation of residual cumulus cells on day 3 increases the accuracy of cell-free DNA detection in spent embryo culture medium

Chen

et al. 2022

J Assist Reprod Genet

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Respirometric reserve capacity of cumulus cell mitochondria correlates with oocyte maturity

Abstract: The oocyte maturity is potentially related to the mitochondria activity of cumulus cells.

Cited by 8 publications

References 52 publications

Reproductive senescence impairs the energy metabolism of human luteinized granulosa cells

Reproductive senescence impairs the energy metabolism of human luteinized granulosa cells

Reproductive Senescence Impairs the Energy Metabolism of Human Granulosa Cells

Re-denudation of residual cumulus cells on day 3 increases the accuracy of cell-free DNA detection in spent embryo culture medium

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