Gender specific differences in the liver proteome of rats exposed to short term and low-concentration hexabromocyclododecane (HBCD)

Abstract

Gender specific impact of HBCD on rat liver proteome, determined by 2D-DIGE.

Dataset of liver proteins of eu- and hypothyroid rats affected in abundance by any of three factors: in vivo exposure to hexabromocyclododecane (HBCD), thyroid status, gender differences

“…The animal experiment was detailed in [1] , [2] and approved under number 2006-051 by the Animal Welfare Committee of Wageningen University. In brief, male Wistar WU (HsdCpbWU) rats with normal or reduced thyroid function (hypothyroid) were orally exposed to 0, 3 or 30 mg/kg bw/d HBCD, respectively, for 7 consecutive days.…”

“…Rat liver lysates were subjected to two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) as previously described, without modifications, to make it compatible to the previously performed study in females [1] , [2] , [3] . This comprised a classical 2D-DIGE experiment, by separating CyDye-labeled proteins from liver lysates in a non-linear 3–10 pH-range and subsequently in large-format SDS-PAGE gels (260×200×1 mm).…”

“…Spots differentially regulated in any of the group comparisons (including samples/gels from males and females) were automatically picked, trypsin digested and proteins identified by MALDI-TOF/TOF 5800 (Sciex) as previously described [1] , [2] , [3] . This also included spots from female rat livers previously not analyzed, as only now they were found regulated in the comparison with male rats׳ data.…”

“…All proteins with significantly changed abundance in any of these comparisons were identified by mass spectrometry. General, hormone and proteomic data of both the present and the previous studies are discussed in Miller et al (2016) [1] and in "Gender specific differences in the liver proteome of rats exposed to hexabromocyclododecane (HBCD)" Miller et al (2016) [2] .…”

Use of proteomics to detect sex-related differences in effects of toxicants: implications for using proteomics in toxicology

“…The greater susceptibility of females than males to develop diabetes has been ascribed to lower insulin secretion, more severe liver damage and larger impairment of regulation of sex hormones as well as lower glucose tolerance and higher blood glucose levels (Chaudhari and Yun 2014). Studies on HBCD and other BFRs were in part based on the fact that for these compounds sex-dependent effects on body and organ weights and on thyroid hormone levels were reported, suggesting that female rats may be more sensitive than males (Miller et al 2016). The study reported by Schwab et al (Schwab et al 2011) on effects of dietary genistein on sex differences in protein abundance in mice was based on the elevated cardiovascular risk in especially postmenopausal women.…”