Fine Mapping of a Novel Heading Date Gene, TaHdm605, in Hexaploid Wheat

Zhang, Xueying; Liu, Guoxiang; Zhang, Lichao; Xia, Chuan; Zhao, Tianxiang; Jia, Jizeng; Xu, Liu; Kong, Xiuying

doi:10.3389/fpls.2018.01059

Cited by 14 publications

(11 citation statements)

References 36 publications

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“…One of important agronomic traits of rice is heading dates, which is related to the attributes of regional adaptability and yield. 18 DJ is a mid-to-late flowering rice cultivar that is generally cultivated in the Korean peninsula (Southern plain region, 33°–44° N latitude). Based on the results, mid-to-late August, which is the summer season in Korea, is suitable for heading of both DJ and I526 rice.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol-enriched rice identical to original Dongjin rice variety with respect to major agronomic traits in different cultivation years and regions

Kantayos

Shin²,

Kim

et al. 2021

GM Crops & Food

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Resveratrol is synthesized by the catalysis of resveratrol synthases (RS) in a limited number of higher plants. Resveratrol shows potential health-promoting properties, including as an antioxidant and in preventing cardiovascular diseases. Recently, resveratrol-enriched rice has been produced as a novel source of resveratrol. This study aimed to investigate the major agronomic characteristics of resveratrol-enriched rice, Iksan526 (I526) and compared them with those of a nontransgenic and commercial rice variety, Dongjin (DJ). Transgene (RS) integration was confirmed using Southern blot analysis, and homologous recombination was achieved after digestion with the SacI restriction enzyme. The phenotypic traits of I526 grown in Iksan were similar to those grown in Milyang but not similar to those grown in Suwon. In Suwon, I526 had slightly earlier heading dates [i.e., number of days from sowing to heading) and shorter culm lengths. When I526 was treated with 0.4% Basta in the seedling stage, no significant difference was observed among all the agronomic traits compared with nontreated I526; particularly, the culm length, panicle length, number of panicles per hill, 1,000 grain weight of brown rice, and brown rice yield of the Basta-treated rice were similar to those of the nontreated I526, regardless of their cultivation region. The resveratrol content of I526 grown in Suwon and Milyang was increased by 18% and 37%, respectively, than that of I526 grown in the Iksan area. Therefore, DJ and I526 are not significantly different in terms of major agronomic traits depending on variety/year and variety/cultivation region. The results indicated that I526 has the potential to become a commercialized variety in the near future.

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Section: Discussionmentioning

confidence: 99%

Resveratrol-enriched rice identical to original Dongjin rice variety with respect to major agronomic traits in different cultivation years and regions

Kantayos

Shin²,

Kim

et al. 2021

GM Crops & Food

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“…m605 is a late heading mutant that was identified from the ethyl methanesulfonate mutant library of YZ4110. Previous studies conducted in our laboratory identified m605 as a late heading mutant compared with its wild type parent YZ4110 [ 32 ]. To reveal the flowering time regulation model of genes for the heading date, homozygotes for the early heading date (WHd) and late heading date phenotype (MHd) (Fig.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome profiling of developing leaf and shoot apices to reveal the molecular mechanism and co-expression genes responsible for the wheat heading date

Yang

Zhang

et al. 2021

BMC Genomics

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Background Wheat is one of the most widely planted crops worldwide. The heading date is important for wheat environmental adaptability, as it not only controls flowering time but also determines the yield component in terms of grain number per spike. Results In this research, homozygous genotypes with early and late heading dates derived from backcrossed progeny were selected to conduct RNA-Seq analysis at the double ridge stage (W2.0) and androgynous primordium differentiation stage (W3.5) of the leaf and apical meristem, respectively. In total, 18,352 differentially expressed genes (DEGs) were identified, many of which are strongly associated with wheat heading date genes. Gene Ontology (GO) enrichment analysis revealed that carbohydrate metabolism, trehalose metabolic process, photosynthesis, and light reaction are closely related to the flowering time regulation pathway. Based on MapMan metabolic analysis, the DEGs are mainly involved in the light reaction, hormone signaling, lipid metabolism, secondary metabolism, and nucleotide synthesis. In addition, 1,225 DEGs were annotated to 45 transcription factor gene families, including LFY, SBP, and MADS-box transcription factors closely related to flowering time. Weighted gene co-expression network analysis (WGCNA) showed that 16, 336, 446, and 124 DEGs have biological connections with Vrn1-5 A, Vrn3-7B, Ppd-1D, and WSOC1, respectively. Furthermore, TraesCS2D02G181400 encodes a MADS-MIKC transcription factor and is co-expressed with Vrn1-5 A, which indicates that this gene may be related to flowering time. Conclusions RNA-Seq analysis provided transcriptome data for the wheat heading date at key flower development stages of double ridge (W2.0) and androgynous primordium differentiation (W3.5). Based on the DEGs identified, co-expression networks of key flowering time genes in Vrn1-5 A, Vrn3-7B, WSOC1, and Ppd-1D were established. Moreover, we discovered a potential candidate flowering time gene, TraesCS2D02G181400. Taken together, these results serve as a foundation for further study on the regulatory mechanism of the wheat heading date.

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“…Plant materials m605 is a late heading mutant that was identi ed from the ethyl methanesulfonate mutant library of YZ4110. Previous studies conducted in our laboratory identi ed m605 as a late heading mutant compared with its wild-type parent YZ4110 [32]. To reveal the molecular mechanism of the genes that regulate the heading date, backcrossed BC 3 F 4 progeny of YZ4110 × m605 was subjected to RNA-seq analysis, and the purpose of using the backcrossed segregating population was to reduce the false positive problems caused by the genetic background.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Profiling of Developing Leaf and Shoot Apices to Reveal the Molecular Mechanism and Coexpression Genes Responsible for the Wheat Heading Date

Yang

Zhang

et al. 2020

Preprint

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Background: Wheat is one of the most widely planted crops worldwide. The heading date is important for wheat environmental adaptability; it not only controls flowering time but also determines the yield component in terms of the grain number per spike. Results: In this research, homozygous genotypes with early and late heading dates derived from backcrossed progeny were selected to conduct RNA-seq analysis at the double ridge stage and androgynous primordium differentiation stage of the leaf and apical meristem, respectively. In total, 18,352 differentially expressed genes (DEGs) were identified. Five common GO terms and 214 common metabolic pathways were obtained by MapMan software. In addition, 1,225 DEGs were annotated to 52 transcription factor gene families, and we discovered that most of the LFY, SBP, and MADS-box genes were highly expressed during the apical development of wheat with the early heading date. Additionally, weighted gene coexpression network analysis (WGCNA) found that the DEGs clustered into 17 coexpression modules, and 16, 336, 446, and 124 DEGs had biological connections with Vrn1-5A, Vrn3-7B, Ppd-1D, and WSOC1, respectively. The 16 genes connected with Vrn1-5A were divided into three types of expression patterns. We further identified one important flowering time gene, TraesCS2D02G181400, which is a MADS-MIKC transcriptional factor and has a biological connection with Vrn1-5A. Gene function showed that this gene was differentially expressed at the A2.0 and L3.5 stages between the genotypes with early and late heading.Conclusions: The RNA-seq analysis results in our present research are a valuable bioinformatics resource for further study of the molecular mechanism of the wheat heading date. Overall, our results shed light on the genetic architecture of wheat flowering time.

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Fine Mapping of a Novel Heading Date Gene, TaHdm605, in Hexaploid Wheat

Cited by 14 publications

References 36 publications

Resveratrol-enriched rice identical to original Dongjin rice variety with respect to major agronomic traits in different cultivation years and regions

Resveratrol-enriched rice identical to original Dongjin rice variety with respect to major agronomic traits in different cultivation years and regions

Transcriptome profiling of developing leaf and shoot apices to reveal the molecular mechanism and co-expression genes responsible for the wheat heading date

Transcriptome Profiling of Developing Leaf and Shoot Apices to Reveal the Molecular Mechanism and Coexpression Genes Responsible for the Wheat Heading Date

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