Molecular detection and genetic diversity of Theileria orientalis in cattle in China

Wang, Jinming; Yang, Jifei; Liu, Junlong; Wang, Xiaoxing; Xu, Jie; Liu, Aihong; Li, Youquan; Liu, Zhijie; Ren, Qiaoyun; Luo, Jianxun; Guan, Guiquan; Yin, Hong

doi:10.1007/s00436-018-6023-3

Cited by 14 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, genotype studies in South Korea have detected Theileria MPSP genotypes 1, 2, 4, and 8 in ticks (2.7%, 15/556) from grazing cattle [19]; types 1-4 and 7 (5.0%, 29/576) in ticks from cattle [15]; types 1, 2, and 7 (17.7%, 12/68) in cattle [10]; and types 1-3 and 7 (41.3%, 57/138) in cattle [9]. In China, types 1-5, 7, N3, and novel N4 of the Theileria MPSP gene (4.5%, 67/1488) were detected in ticks [20] and types 1-5, 7, N1, and N2 of the Theileria MPSP gene (36.5%, 95/260) were detected in cattle [21]. In this study, types 1-3, 7, and N3 of the Theileria MPSP gene (1.2%, 9/737) were detected in cattle.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Bovine Hemoprotozoa in South Korea

Kwak

Seo

2020

Pathogens

View full text Add to dashboard Cite

Tick-borne pathogens cause economically significant diseases in cattle. Theileria spp. are parasitic protozoa and the causative agent of bovine theileriosis. Here we report the distribution and risk factors of bovine Theileria using blood samples taken between 2018 and 2019. Of 737 tested cattle, nine animals (1.2%) were positive for Theileria orientalis infection by 18S rRNA gene amplification. Further analysis of the infected samples using the T. orientalis major piroplasm surface protein (MPSP) gene revealed five different genotypes circulating in the population: Types 1, 2, 3, 7, and N3. To the best of our knowledge, this is the first research to describe the existence of the T. orientalis MPSP genotype N3 in South Korea. Although the prevalence of bovine T. orientalis was low, our study offers data on the geographical distribution and prevalence of bovine Theileria spp. in South Korea. Further studies are warranted to determine the correlation of clinical symptoms with parasite MPSP genotypes. Our data provide epidemiological information to help control bovine theileriosis in South Korea.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Bovine Hemoprotozoa in South Korea

Kwak

Seo

2020

Pathogens

View full text Add to dashboard Cite

show abstract

“…When multiple B. bovis strains with distinct MSA-1 variants are transmitted by vector ticks, a new variant could probably be generated within ticks' mid-gut via genetic exchange, which might be the reason for the emergence and expansion of new variants in cattle populations [32]. The clinical significance of infection with distinct B. bovis variants has not been recorded; however, it was confirmed that several variants of T. orientalis and B. rossi were closely associated with outbreaks of theileriosis and babesiosis, respectively [35][36][37]. The relationship between genetic variants in the MSA genes and clinical status remains unknown, and requires investigation.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

et al. 2020

Self Cite

View full text Add to dashboard Cite

The apicomplexan parasite Babesia bovis is a tick-borne intracellular hemoprotozoan parasite that is widespread across China. Genetic diversity is an important strategy used by parasites to escape the immune responses of their hosts. In our present study, 575 blood samples, collected from cattle in 10 provinces, were initially screened using a nested PCR (polymerase chain reaction) for detection of B. bovis infection. To perform genetic diversity analyses, positive samples were further amplified to obtain sequences of three B. bovis merozoite surface antigen genes (MSA-1, MSA-2b, MSA-2c). The results of the nested PCR approach showed that an average of 8.9% (51/575) of cattle were positive for B. bovis infection. Phylogenetic analyses of the predicted amino acid sequences revealed that unique antigen variants were formed only by Chinese isolates. Our findings provide vital information for understanding the genetic diversity of B. bovis in China.

show abstract

“…From recently published reports, Ikeda/type 2 T. orientalis is the most common type responsible for outbreaks of the disease in Australia, New Zealand, and the United States [20,36,37]. In China, many types, including type 1 (Chitose) and 2 (Ikeda), have been con rmed [24,27,29] by analyzing blood samples of grazing cattle or ticks. However, few clinical cases related to T. orientalis have been reported.…”

Section: Discussionmentioning

confidence: 99%

“…In China, T. orientalis is the most prevalent Theileria species and is distributed in at least 20 provinces [23][24][25][26]. Based on MPSP analysis, 10 genotypes, including types 1-5, 7, 9 and N1-3, have been con rmed to infect beef or dairy cattle and yaks [24,[27][28][29]. However, all the genotypes of T. orientalis found in China have been identi ed from eld-collected blood samples or ticks, and no report has addressed the pathogenicity of the identi ed T. orientalis genotypes.…”

Section: Introductionmentioning

confidence: 99%

Detection and isolation of pathogenic Theileria orientalis Ikeda genotype from confined dairy cattle, in Hebei, China

Liu

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background Theileria orientalis is a group of benign pathogenic of cattle parasites with a cosmopolitan distribution, and has been classified into 11 genotypes through MPSP gene phylogenetic analysis. In China, T. orientalis is the most prevalent Theileria species, with several genotypes, but few fatal cases have been reported. In June 2020, dairy cattle in Zhangjiakou, Hebei Province, showed clinical symptoms of piroplasmosis, causing many animals to die. In the present study, we confirmed the infection of T. orientalis was responsible for the disease and isolated the T. orientalis Ikeda genotype from the field blood samples. Methods Fifty-one blood samples were collected from ill and recovered animal. Meanwhile, 12 samples were collected from animals without any clinical symptoms. Blood smears were prepared from blood samples and detected with microscope. Genomic DNA was extracted and tested by PCR method. The MPSP gene was sequenced from the Theileria positive samples detected by both methods, to identify the T. orientalis genotypes. Parasites were isolated by inoculate the mixed positive blood samples into the experimental cattle and identified by MPSP sequence analysis. Results Blood smears and PCR detection results confirmed T. orientalis infection with a 66.7% positive rate of collected blood samples. The MPSP sequences analysis revealed parasite genotypes 1 (Chitose) and 2 (Ikeda). In animal experiments, only T. orientalis genotype 2 (Ikeda) was obtained, indicating that the Ikeda type was predominant and responsible for the disease. Conclusions The present study firstly identified pathogenic T. orientalis Ikeda in dairy cattle in China, and isolated the parasite from experimental animal. Although many T. orientalis genotypes are present in China, the possibility of T. orientalis genotype 1 and 2 infections in confined dairy cattle should considered to avoid additional economic losses.

show abstract

Molecular detection and genetic diversity of Theileria orientalis in cattle in China

Cited by 14 publications

References 29 publications

Genetic Diversity of Bovine Hemoprotozoa in South Korea

Genetic Diversity of Bovine Hemoprotozoa in South Korea

Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

Detection and isolation of pathogenic Theileria orientalis Ikeda genotype from confined dairy cattle, in Hebei, China

Contact Info

Product

Resources

About