Dual range lactate oxidase-based screen printed amperometric biosensor for analysis of lactate in diversified samples

Cunha-Silva, Hugo; Arcos‐Martínez, M. Julia

doi:10.1016/j.talanta.2018.06.054

Cited by 34 publications

(15 citation statements)

References 75 publications

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“…It was found to be 8.6%. These properties compare well with other recently published L-lactate electrochemical biosensors based on LOx, summarized in Table 1 [44][45][46][47][48][49][50], with the advantage of this being simpler and prepared with low cost materials.…”

Section: Biosensor Responsesupporting

confidence: 85%

Enhanced Performance of Reagent-Less Carbon Nanodots Based Enzyme Electrochemical Biosensors

Bravo

Gutiérrez-Sánchez

García‐Mendiola

et al. 2019

Sensors

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This work reports on the advantages of using carbon nanodots (CNDs) in the development of reagent-less oxidoreductase-based biosensors. Biosensor responses are based on the detection of H2O2, generated in the enzymatic reaction, at 0.4 V. A simple and fast method, consisting of direct adsorption of the bioconjugate, formed by mixing lactate oxidase, glucose oxidase, or uricase with CNDs, is employed to develop the nanostructured biosensors. Peripherical amide groups enriched CNDs are prepared from ethyleneglycol bis-(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid and tris(hydroxymethyl)aminomethane, and used as precursors. The bioconjugate formed between lactate oxidase and CNDs was chosen as a case study to determine the analytical parameters of the resulting L-lactate biosensor. A linear concentration range of 3.0 to 500 µM, a sensitivity of 4.98 × 10−3 µA·µM−1, and a detection limit of 0.9 µM were obtained for the L-lactate biosensing platform. The reproducibility of the biosensor was found to be 8.6%. The biosensor was applied to the L-lactate quantification in a commercial human serum sample. The standard addition method was employed. L-lactate concentration in the serum extract of 0.9 ± 0.3 mM (n = 3) was calculated. The result agrees well with the one obtained in 0.9 ± 0.2 mM, using a commercial spectrophotometric enzymatic kit.

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Section: Biosensor Responsesupporting

confidence: 85%

Enhanced Performance of Reagent-Less Carbon Nanodots Based Enzyme Electrochemical Biosensors

Bravo

Gutiérrez-Sánchez

García‐Mendiola

et al. 2019

Sensors

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“…There are many L-lactate selective electrochemical biosensors described recently. Most of them are based on the usage of three enzymes, one of mammalian origin—NAD + -dependent lactate dehydrogenase (EC: 1.1.1.27, LDH) [ 3 , 4 ] and two microbially produced: L-lactate: cytochrome c oxidoreductase (EC: 1.1.2.3, flavocytochrome b 2 , FC b 2 ) [ 5 , 6 , 7 ] and lactate oxidase (EC: 1.13.12.4, LOx) [ 8 , 9 , 10 ]. Usually, the enzymes are used separately, but sometimes LOx and LDH could be combined into one biosensing element [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Biosensor Based on Peroxidase-Mimetic Nanozyme and Lactate Oxidase for Accurate L-Lactate Analysis in Beverages

et al. 2022

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Precision analysis of the key biological metabolites such as L-lactate has great practical importance for many technological processes in food technology, including beverage production. Here we describe a new, highly selective, and sensitive biosensor for accurate L-lactate assay based on a combination of peroxidase-mimetic nanozymes with microbial lactate oxidase (LOx) immobilized onto the surface of a graphite-rod electrode (GE). The peroxidase-like nanozymes were synthesized using the debris of carbon microfibers (CFs) functionalized with hemin (H) and modified with gold nanoparticles (AuNPs) or platinum microparticles (PtMPs). The nanozyme formed with PtMPs as well as corresponding bioelectrodes based on it (LOx-CF-H-PtMPs/GE) is characterized by preferable catalytic and operational characteristics, so it was selected for the analysis of L-lactate content in real samples of grape must and red wine. The results of the L-lactate analysis obtained by the developed biosensors are highly correlated with a very selective spectrophotometric approach used as a reference. The developed biosensor, due to its high selectivity and sensitivity, is very prospective not only for the beverage industry and food technology, but also for clinical diagnostics and medicine, as well as in other applications where the accurate analysis of L-lactate is highly important.

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“…Many lactate sensors, including electrochemical lactate sensors and optical lactate sensors, have been reviewed by L. Rassaei et al [13] and K. Rathee et al [14]. Several new concepts have recently been developed for the fabrication of lactate sensors for noninvasive measurements, such as wearable sensor systems to detect lactate in sweat [15], screen-printed lactate electrodes [16,17], self-powered lactate sensors [18], and test strip lactate sensors [19]. Similar to fluorescence glucose biosensors, fluorescent lactate biosensors are also of great interest because they show great benefits through the specific interaction between fluorescence dyes and substrates.…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane

Duong

Rhee

2021

Biosensors

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In this study, ratiometric fluorescent glucose and lactate biosensors were developed using a ratiometric fluorescent oxygen-sensing membrane immobilized with glucose oxidase (GOD) or lactate oxidase (LOX). Herein, the ratiometric fluorescent oxygen-sensing membrane was fabricated with the ratio of two emission wavelengths of platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) doped in polystyrene particles and coumarin 6 (C6) captured into silica particles. The operation mechanism of the sensing membranes was based on (i) the fluorescence quenching effect of the PtP dye by oxygen molecules, and (ii) the consumption of oxygen levels in the glucose or lactate oxidation reactions under the catalysis of GOD or LOX. The ratiometric fluorescent glucose-sensing membrane showed high sensitivity to glucose in the range of 0.1–2 mM, with a limit of detection (LOD) of 0.031 mM, whereas the ratiometric fluorescent lactate-sensing membrane showed the linear detection range of 0.1–0.8 mM, with an LOD of 0.06 mM. These sensing membranes also showed good selectivity, fast reversibility, and stability over long-term use. They were applied to detect glucose and lactate in artificial human serum, and they provided reliable measurement results.

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Dual range lactate oxidase-based screen printed amperometric biosensor for analysis of lactate in diversified samples

Cited by 34 publications

References 75 publications

Enhanced Performance of Reagent-Less Carbon Nanodots Based Enzyme Electrochemical Biosensors

Enhanced Performance of Reagent-Less Carbon Nanodots Based Enzyme Electrochemical Biosensors

Biosensor Based on Peroxidase-Mimetic Nanozyme and Lactate Oxidase for Accurate L-Lactate Analysis in Beverages

Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane

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