Liquid Metal‐Based Multifunctional Micropipette for 4D Single Cell Manipulation

Chow, Y. T.; Man, Tianxing; Acosta‐Vélez, Giovanny F.; Zhu, Xue‐Feng; Wen, Ximiao; Chung, Pei‐Shan; Liu, Tingyi; Wu, Benjamin M.; Chiou, Pei‐Yu

doi:10.1002/advs.201700711

Cited by 28 publications

(15 citation statements)

References 38 publications

(41 reference statements)

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“…The cell can be rotated in 3D by applying appropriate AC signals to the electrodes. As shown in Figure 6 B , Yu et al 63 prepared a capillary microneedle electrode based on liquid metal by injecting liquid metal gallium into a multitube glass capillary. By applying multifrequency, multiamplitude, multiphase AC electrical signals to the microelectrodes, three-dimensional dielectric electrophoresis traps and one-dimensional electrical rotation can be generated simultaneously, realizing 4D single-cell manipulation.…”

Section: Oocyte Micromanipulationmentioning

confidence: 99%

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Recent advances in critical nodes of embryo engineering technology

Ma¹,

Gu²,

Chen³

et al. 2021

Theranostics

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The normal development and maturation of oocytes and sperm, the formation of fertilized ova, the implantation of early embryos, and the growth and development of foetuses are the biological basis of mammalian reproduction. Therefore, research on oocytes has always occupied a very important position in the life sciences and reproductive medicine fields. Various embryo engineering technologies for oocytes, early embryo formation and subsequent developmental stages and different target sites, such as gene editing, intracytoplasmic sperm injection (ICSI), preimplantation genetic diagnosis (PGD), and somatic cell nuclear transfer (SCNT) technologies, have all been established and widely used in industrialization. However, as research continues to deepen and target species become more advanced, embryo engineering technology has also been developing in a more complex and sophisticated direction. At the same time, the success rate also shows a declining trend, resulting in an extension of the research and development cycle and rising costs. By studying the existing embryo engineering technology process, we discovered three critical nodes that have the greatest impact on the development of oocytes and early embryos, namely, oocyte micromanipulation, oocyte electrical activation/reconstructed embryo electrofusion, and the in vitro culture of early embryos. This article mainly demonstrates the efforts made by researchers in the relevant technologies of these three critical nodes from an engineering perspective, analyses the shortcomings of the current technology, and proposes a plan and prospects for the development of embryo engineering technology in the future.

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Section: Oocyte Micromanipulationmentioning

confidence: 99%

“…(C) The effect of dielectrophoresis forceps and drive oocyte rotation. Adapted with permission from 62 , copyright 2018 Royal Society of Chemistry, and 63 , copyright 2018 John Wiley and Sons, and 64 , copyright 2020 IEEE.…”

Section: Figurementioning

confidence: 99%

Recent advances in critical nodes of embryo engineering technology

Ma¹,

Gu²,

Chen³

et al. 2021

Theranostics

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“…Pt microelectrodes for high sensitivity electrochemical sensing were fabricated on small, thin silicon dioxide islands on a separate silicon substrate and transferred onto the ULTS probe. To provide electrical connections to these electrodes on the flexible and stretchable probe without cracking and delamination issues, gallium (Ga), a liquid metal, was used due to its fluidity, high electrical conductivity, and biocompatability (Chow et al, 2018;Dickey, 2017;Hallfors et al, 2013).…”

Section: Ults Probe Design and Working Principlementioning

confidence: 99%

Flexible, multifunctional neural probe with liquid metal enabled, ultra-large tunable stiffness for deep-brain chemical sensing and agent delivery

Wen

Wang

Huang

et al. 2019

Biosensors and Bioelectronics

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124

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Flexible neural probes have been pursued previously to minimize the mechanical mismatch between soft neural tissues and implants and thereby improve long-term performance. However, difficulties with insertion of such probes deep into the brain severely restricts their utility. We describe a solution to this problem using gallium (Ga) in probe construction, taking advantage of the solid-to-liquid phase change of the metal at body temperature and probe shape deformation to provide temperature-dependent control of stiffness over 5 orders of magnitude. Probes in the stiff state were successfully inserted 2 cm-deep into agarose gel "brain phantoms" and into rat brains under cooled conditions where, upon Ga melting, they became ultra soft, flexible, and stretchable *

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“…However, this material has not been introduced into microfluidics chip as a microelectrode. The use of Galinstan liquid metal as an electrode in a microchannel is feasible because of its excellent electrical conductivity and large surface tension [35]- [37].…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Microfluidic Chip Integrated With Liquid Metal Electrode for Red Blood Cell Stretching Manipulation

Zhu

Cai

et al. 2019

IEEE Access

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Cellular mechanical properties are closely related to cell physiological functions and status, and their analysis and measurement help understand cell mechanism. In this study, a microfluidic platform was built to measure the mechanical properties of cells by using dielectrophoretic (DEP) force. The electrodes generally used to stretch cells are made of indium tin oxide, Au, and Pt, which have inherent disadvantages. In this paper, galinstan alloy liquid metal was first introduced as microelectrode to form non-uniform electric filed for red blood cell stretching manipulation. The liquid metal microelectrode is easy to manufacture, low in price, stable at high voltage, and reusable. An effective microfluidic chip integrated with liquid metal electrode was designed and simulated, and a series of experiments to capture and stretch red blood cells was performed. The length of the red blood cells increased from 6 µm to 8 µm under the DEP force from 0 pN to 103 pN. This work also revealed the potential use of liquid metal as microelectrode to manipulate the microparticles and cells in a microfluidic chip. INDEX TERMS Liquid metal electrode, galinstan, dielectrophoresis, microfluidic chip, cell stretching.

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Liquid Metal‐Based Multifunctional Micropipette for 4D Single Cell Manipulation

Cited by 28 publications

References 38 publications

Recent advances in critical nodes of embryo engineering technology

Recent advances in critical nodes of embryo engineering technology

Flexible, multifunctional neural probe with liquid metal enabled, ultra-large tunable stiffness for deep-brain chemical sensing and agent delivery

Dielectrophoretic Microfluidic Chip Integrated With Liquid Metal Electrode for Red Blood Cell Stretching Manipulation

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