Expression of transmembrane protein 41A is associated with metastasis via the modulation of E‑cadherin in radically resected gastric cancer

Lin, Bin; Xue, Yingming; Qi, Chaorong; Chen, Xiangjie; Mao, Weizheng

doi:10.3892/mmr.2018.9241

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…We recognized that TMEM41A is upregulated in gastric cancer, especially metastatic and advanced tumors, and contributed to poor prognosis. In vitro and in vivo assay showed that TMEM41A has effects on the migration ability of gastric cancer (35). We investigated expression of TMEM41A with our RNA-sequencing data; however, there were no significant differences in the expression level.…”

Section: Discussionmentioning

confidence: 99%

HOXC10 overexpression promotes cell proliferation and migration in gastric cancer

et al. 2019

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Homeodomain-containing gene 10 ( HOXC10 ) is a member of the homeobox transcription factors that plays an important role in the development of multicellular organisms. HOXC10 is overexpressed in a variety of human cancers, and recent studies have revealed that HOXC10 is upregulated in gastric cancer as well. However, its mechanism of action is not fully understood, thus, the role of HOXC10 was investigated in the present study in human gastric cancer. First, HOXC10 expression was revealed to be significantly increased in gastric cancer tissues compared to normal tissues (TCGA dataset), and HOXC10 upregulation was associated with decreased recurrence-free survival in gastric cancer patients in a public gene expression dataset. HOXC10 promoted cell proliferation and metastasis in two gastric cancer cell lines (AGS and MKN74). Analyzing TCGA 450K DNA methylation dataset, it was revealed that HOXC10 CpG sites were hypomethylated in gastric cancer tissues. Bisulfite sequencing revealed that CpG sites in the HOXC10 first intronic region were hypomethylated in three gastric cancer tissues, and HOXC10 expression was increased in gastric cancer cell lines (AGS and SNU620) in response to 5-azacytidine treatment. By RNA-sequencing of AGS cells with ectopic HOXC10 expression, it was revealed that many genes were upregulated by HOXC10 overexpression. Among them, CST1 was predicted to be a HOXC10 direct target gene via prediction of HOXC10 binding sites from the JASPAR database. A chromatin immunoprecipitation assay revealed that HOXC10 directly bound to CST1 promoter regions. The present study proposes HOXC10 is a potential prognostic marker or therapeutic target in human gastric cancer.

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Section: Discussionmentioning

confidence: 99%

HOXC10 overexpression promotes cell proliferation and migration in gastric cancer

et al. 2019

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“…SREBF1 is associated with p65 phosphorylation [23,24]. Knockdown of TMEM41A was also observed to affect gastric cancer metastasis via the modulation of E-cadherin [25]. Colorectal carcinoma chemoresistance to gemcitabine was induced by SREBP-1-mediated lipogenesis and STAT3 activation in CRC [26].…”

Section: Discussionmentioning

confidence: 94%

Chromosome 12 Open Reading Frame 49 Promotes Tumor Growth and Predicts Poor Prognosis in Colorectal Cancer

et al. 2022

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Background and AimsLittle is known about the role of chromosome 12 open reading frame 49 (C12ORF49)-induced metabolic signal transduction in tumor growth. We investigated the relationship between C12ORF49 expression and prognosis in colorectal cancer (CRC) patients. Methods C12ORF49 protein expression was measured in CRC tissues by Western blot and immunohistochemistry staining. Knock out of C12ORF49 in CRC cells was then performed, and the role of C12ORF49 in CRC cell proliferation and growth was examined. The expression of C12ORF49 in CRC was analyzed in Gene Expression Profiling Interactive Analysis (GEPIA) databases. A prognosis model with 11 C12ORF49-associated genes (CAGs) was generated by TCGA databases. Results C12ORF49 expression was significantly higher in CRC tumor tissue than in non-tumor tissue. Furthermore, in vitro and in vivo loss-of-function experiments, showed that C12ORF49 plays critical roles in promoting tumor cell growth. There was a significant correlation between C12ORF49 protein and the presence of tumor necrosis. C12ORF49 is critical for its interaction with SREBF1, TMEM41A, and S1PR3 in the poor prognosis of CRC. Conclusions Our results suggest that C12ORF49 plays a key role in CRC tumor growth.

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“…Cancer involves a complex regulatory network and the integration of multiple biomarkers into an aggregation model can improve the prognostic value compared with a single biomarker ( 46 ). For example, the expression of transmembrane protein 41A is associated with metastasis by modulating E-cadherin in radically resected gastric cancer ( 47 ). In the present study, 24 hub genes were identified by overlapping the top 30 genes within four local-based methods (DMNC, MNC, MCC and degree), in the Cytoscape plug-in cytoHubba.…”

Section: Discussionmentioning

confidence: 99%

Identification of glioblastoma‑specific prognostic biomarkers via an integrative analysis of DNA methylation and gene expression

2020

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Glioblastoma (GBM) is the most aggressive and lethal tumor of the central nervous system. The present study set out to identify reliable prognostic and predictive biomarkers for patients with GBM. RNA-sequencing data were obtained from The Cancer Genome Atlas database and DNA methylation data were downloaded using the University of California Santa Cruz-Xena database. The expression and methylation differences between patients with GBM, and survival times <1 and ≥1 year were investigated. A protein-protein interaction network was constructed and functional enrichment analyses of differentially expressed and methylated genes were performed. Hub genes were identified using the Cytoscape plug-in cytoHubba software. Survival analysis was performed using the survminer package, in order to determine the prognostic values of the hub genes. The present study identified 71 genes that were hypomethylated and expressed at high levels, and four genes that were hypermethylated and expressed at low levels in GBM. These genes were predominantly enriched in the 'JAK-STAT signaling pathway', 'transcriptional misregulation in cancer' and the 'ECM-receptor interaction', which are associated with GBM development. Among the 24 hub genes identified, 15 possessed potential prognostic value. An integrative analysis approach was implemented in order to analyze the association of DNA methylation with changes in gene expression and to assess the association of gene expression changes with GBM survival time. The results of the present study suggest that these 15 CpG-based genes may be useful and practical tools in predicting the prognosis of patients with GBM. However, future research on gene methylation and/or expression is required in order to develop personalized treatments for patients with GBM.

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Expression of transmembrane protein 41A is associated with metastasis via the modulation of E‑cadherin in radically resected gastric cancer

Cited by 8 publications

References 32 publications

HOXC10 overexpression promotes cell proliferation and migration in gastric cancer

HOXC10 overexpression promotes cell proliferation and migration in gastric cancer

Chromosome 12 Open Reading Frame 49 Promotes Tumor Growth and Predicts Poor Prognosis in Colorectal Cancer

Identification of glioblastoma‑specific prognostic biomarkers via an integrative analysis of DNA methylation and gene expression

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