Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli

Ranfaing, J; Dunyach-Rémy, Catherine; Louis, Laurence; Lavigne, Jean‐Philippe; Sotto, Albert

doi:10.1038/s41598-018-29082-6

Cited by 21 publications

(25 citation statements)

References 72 publications

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“…Propolis has antimicrobial, anti-inflammatory, anti-tumoral, immunemodulatory and anti-oxidant activities (Boonsai et al, 2014). It has been used for several years to treat gastrointestinal disorders (food supplement) (Freitas et al, 2006), to promote oral health (mouthwash) (Pereira et al, 2011) and in dermatological care (creams and ointments) (Olczyk et al, 2014;Pasupuleti et al, 2017;Ranfaing et al, 2018b). In vitro studies showed that propolis potentiated the effect of cranberry proanthocyanidins on adhesion, motility (swarming and swimming), biofilm formation (early formation and fully-formed biofilm), iron metabolism and stress response of UPEC (Olczyk et al, 2014;Pasupuleti et al, 2017;Ranfaing et al, 2018a,b).…”

Section: Cranberry (Vaccinium Macrocarpon)mentioning

confidence: 99%

“…It has been used for several years to treat gastrointestinal disorders (food supplement) ( Freitas et al, 2006 ), to promote oral health (mouthwash) ( Pereira et al, 2011 ) and in dermatological care (creams and ointments) ( Olczyk et al, 2014 ; Pasupuleti et al, 2017 ; Ranfaing et al, 2018b ). In vitro studies showed that propolis potentiated the effect of cranberry proanthocyanidins on adhesion, motility (swarming and swimming), biofilm formation (early formation and fully-formed biofilm), iron metabolism and stress response of UPEC ( Olczyk et al, 2014 ; Pasupuleti et al, 2017 ; Ranfaing et al, 2018a , b ). Moreover, this association was active in all the E. coli strains studied, ruling out the variable effect observed with the cranberry used alone ( Ranfaing et al, 2018a ).…”

Section: Nutraceuticalsmentioning

confidence: 99%

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Alternative Therapeutic Options to Antibiotics for the Treatment of Urinary Tract Infections

et al. 2020

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Urinary tract infections (UTIs) mainly caused by Uropathogenic Escherichia coli (UPEC), are common bacterial infections. Many individuals suffer from chronically recurring UTIs, sometimes requiring long-term prophylactic antibiotic regimens. The global emergence of multi-drug resistant uropathogens in the last decade underlines the need for alternative non-antibiotic therapeutic and preventative strategies against UTIs. The research on non-antibiotic therapeutic options in UTIs has focused on the following phases of the pathogenesis: colonization, adherence of pathogens to uroepithelial cell receptors and invasion. In this review, we discuss vaccines, small compounds, nutraceuticals, immunomodulating agents, probiotics and bacteriophages, highlighting the challenges each of these approaches face. Most of these treatments show interesting but only preliminary results. Lactobacillus-containing products and cranberry products in conjunction with propolis have shown the most robust results to date and appear to be the most promising new alternative to currently used antibiotics. Larger efficacy clinical trials as well as studies on the interplay between non-antibiotic therapies, uropathogens and the host immune system are warranted.

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Section: Cranberry (Vaccinium Macrocarpon)mentioning

confidence: 99%

Section: Nutraceuticalsmentioning

confidence: 99%

Alternative Therapeutic Options to Antibiotics for the Treatment of Urinary Tract Infections

et al. 2020

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“…In vivo studies proved that cranberry proanthocyanidins (190 μg/ml) have a relationship to adhesion, motility, biofilm formation, and iron and stress response of uropathogenic Escherichia coli. Furthermore, cranberry proanthocyanidins influence the transcriptional profiles of Escherichia coli, anti-adhesion effect is mainly the effect of proanthocyanidins on “strategic” genes involved in E. coli adherence [85].…”

Section: Biological Activities Of European Cranberrymentioning

confidence: 99%

Bioactive Compounds, Antioxidant Activity, and Biological Effects of European Cranberry (Vaccinium oxycoccos)

et al. 2018

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Lesser known fruits or underutilized fruit species are recently of great research interest due to the presence of phytochemicals that manifest many biological effects. European cranberry, Vaccinium oxycoccos fruit, as an important representative of this group, is a valuable source of antioxidants and other biologically active substances, similar to American cranberry (V. macrocarpon) which is well known and studied. European cranberry fruit is rich especially in polyphenolic compounds anthocyanins (12.4–207.3 mg/100 g fw), proanthocyanins (1.5–5.3 mg/100 g fw), and flavonols, especially quercetin (0.52–15.4 mg/100 g fw), which mostly contribute to the antioxidant activity of the fruit. Small cranberry is also important due to its various biological effects such as urinary tract protection (proanthocyanidins), antibacterial and antifungal properties (quercetin, proanthocyanidins, anthocyanins), cardioprotective (proanthocyanidins) and anticancer activities (proanthocyanidins), and utilization in food (juice drinks, jams, jellies, sauces, additive to meat products) and pharmacological industries, and in folk medicine.

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“…Accordingly, the condensed tannins of cranberry extracts most commonly known as proanthocyanidins (cPACs) has been reported to reduce the hydrophobicity of bacterial cell surfaces and interfere with their attachment to host cellular or biomaterial surfaces [45]. A similar anti-motility trend was also observed with cranberry pomace extracts against Enterococcus faecalis , Escherichia coli , Pseudomonas aeruginosa and Proteus mirabilis [45–47].…”

Section: Discussionmentioning

confidence: 95%

Transcriptional profiling of Salmonella enterica serovar Enteritidis exposed to ethanolic extract of organic cranberry pomace

Das

Lepp

et al. 2019

PLoS ONE

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Non-typhoidal Salmonella enterica serovars continue to be an important food safety issue worldwide. Cranberry ( Vaccinium macrocarpon Ait ) fruits possess antimicrobial properties due to their various acids and phenolic compounds; however, the underlying mechanism of actions is poorly understood. We evaluated the effects of cranberry extracts on the growth rate of Salmonella enterica serovars Typhimurium, Enteritidis and Heidelberg and on the transcriptomic profile of Salmonella Enteritidis to gain insight into phenotypic and transcriptional changes induced by cranberry extracts on this pathogen. An ethanolic extract from cranberry pomaces (KCOH) and two of its sub-fractions, anthocyanins (CRFa20) and non-anthocyanin polyphenols (CRFp85), were used. The minimum inhibitory (MICs) and bactericidal (MBCs) concentrations of these fractions against tested pathogens were obtained using the broth micro-dilution method according to the Clinical Laboratory Standard Institute’s guidelines. Transcriptional profiles of S . Enteritidis grown in cation-adjusted Mueller-Hinton broth supplemented with or without 2 or 4 mg/ml of KCOH were compared by RNASeq to reveal gene modulations serving as markers for biological activity. The MIC and MBC values of KCOH were 8 and 16 mg/mL, respectively, against all tested S . enterica isolates. The MIC value was 4 mg/mL for both CRFa20 and CRFp85 sub-fractions, and a reduced MBC value was obtained for CRFp85 (4 mg/ml). Treatment of S . Enteritidis with KCOH revealed a concentration-dependent transcriptional signature. Compared to the control, 2 mg/ml of KCOH exposure resulted in 89 differentially expressed genes (DEGs), of which 53 and 36 were downregulated and upregulated, respectively. The upregulated genes included those involved in citrate metabolism, enterobactin synthesis and transport, and virulence. Exposure to 4 mg/ml KCOH led to the modulated expression of 376 genes, of which 233 were downregulated and 143 upregulated, which is 4.2 times more DEGs than from exposure to 2 mg/ml KCOH. The downregulated genes were related to flagellar motility, Salmonella Pathogenicity Island-1 (SPI-1), cell wall/membrane biogenesis, and transcription. Moreover, genes involved in energy production and conversion, carbohydrate transport and metabolism, and coenzyme transport and metabolism were upregulated during exposure to 4 mg/ml KCOH. Overall, 57 genes were differentially expressed (48 downregulated and 9 upregulated) in response to both concentrations. Both concentrations of KCOH downregulated expression of hilA , which is a major SPI-1 transcriptional regulator. This study provides information on the response of Salmonella exposed to cranberry extracts, which could be used in the control of this important foodborne pathogen.

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Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli

Cited by 21 publications

References 72 publications

Alternative Therapeutic Options to Antibiotics for the Treatment of Urinary Tract Infections

Alternative Therapeutic Options to Antibiotics for the Treatment of Urinary Tract Infections

Bioactive Compounds, Antioxidant Activity, and Biological Effects of European Cranberry (Vaccinium oxycoccos)

Transcriptional profiling of Salmonella enterica serovar Enteritidis exposed to ethanolic extract of organic cranberry pomace

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