Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella)

Chu, Pengfei; He, Libo; Li, Yangyang; Huang, Rong; Liao, Lanjie; Li, Yongming; Zhang, Zhu; Wang, Yaping

doi:10.1016/j.fsi.2018.07.031

Cited by 23 publications

(4 citation statements)

References 38 publications

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“…In grass carp, similar to mammals, NLRX1 attenuates innate immunity by interacting with TRAF6. In the same study, enhanced gene expression of IRF3, IRF7, and IFN-I following poly (I:C) stimulation has been observed in NLRX1 deficient grass carp kidney cell line [173] . In black carp, direct association of the NACHT domain of NLRX1 with MAVS has been seen as critical for negative regulation of antiviral signaling [174] .…”

Section: Downstream Signaling Mechanism Of Nlrsmentioning

confidence: 54%

Teleost NOD-like receptors and their downstream signaling pathways: A brief review

Chuphal

Rai²,

Roy³

2022

Fish and Shellfish Immunology Reports

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Section: Downstream Signaling Mechanism Of Nlrsmentioning

confidence: 54%

Teleost NOD-like receptors and their downstream signaling pathways: A brief review

Chuphal

Rai²,

Roy³

2022

Fish and Shellfish Immunology Reports

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“…Melting curves were performed from 60 to 95°C to validate the specificity of PCRs (Palazzotto et al, 2015). The β-actin gene was used as internal control gene (Chu et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

A New Isolate of Pediococcus pentosaceus (SL001) With Antibacterial Activity Against Fish Pathogens and Potency in Facilitating the Immunity and Growth Performance of Grass Carps

Gong

et al. 2019

Front. Microbiol.

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Probiotic-feeding continues to be a promising strategy to control the bacterial pathogens in aquaculture. A new Pediococcus pentosaceus strain (SL001) was isolated from 1000s of soil samples, which exhibited wide antimicrobial spectrum of against fish pathogens, involving Aeromonas hydrophila , Aeromonas veronii , Aeromonas sobria , Edwardsiella tarda , Lactococcus garvieae , and Plesiomonas shigelloide . The challenge test against A. hydrophila showed that the survival rate of SL001-supplemented group was significantly higher than that of control group ( P < 0.05). Moreover, SL001 could stably colonize in gut of grass carp and increased mucus-secreting goblet cells and extended intestinal villi could be observed in SL001-supplemented group ( P < 0.05). Feeding with SL001 supplemented diet could significantly enhance the growth rate ( P < 0.05) and markedly affect gut microbiota structure of grass carps, resulting in reduced potential pathogens and increased potential probiotics. Furthermore, feeding grass carps with SL001 caused the up-regulated expression of insulin-like growth factor (IGF-1 and IGF-2) and down-regulated expression of myostatin (MSTN-1 and MSTN-2) ( P < 0.05), which probably also account for the increased growth rate of SL001-fed group. Meanwhile, relative mRNA expression levels of immune-related genes in liver, spleen, and head kidney were analyzed in grass carps after feeding for 30 days with SL001 supplemented diets. In all three immune organs, the expression levels of immunoglobulin M (IgM) and complement 3 (C3) were significantly increased ( P < 0.05), whereas the interleukin-8 (IL-8) was down-regulated ( P < 0.05). Besides, whole genome sequencing revealed several probiotics properties of SL001, including organic acid synthesis, bacteriocin synthesis (coagulin), superoxide dismutase, and digestive enzymes. In conclusion, P. pentosaceus SL001 which could enhance immunity and promoter growth rate of grass carps, is prospective to be used as a dietary probiotic in freshwater fish aquaculture.

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“…Additionally, CIK cells were transfected with FLAG-ATG5 or empty vectors for 24 h, as described above [ 32 ], followed by GCRV infection. Cells were sampled at 0, 6, 12, and 24 h after GCRV infection (named ATG5-H0, ATG5-H6, ATG5-H12, ATG5-H24, VT-HO, VT-H6, VT-H12, and VT-H24, respectively; three biological duplicates for each group).…”

Section: Methodsmentioning

confidence: 99%

Autophagy Inhibits Grass Carp Reovirus (GCRV) Replication and Protects Ctenopharyngodon idella Kidney (CIK) Cells from Excessive Inflammatory Responses after GCRV Infection

Chu

Huang

et al. 2020

Biomolecules

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Autophagy is an essential and highly conserved process in mammals, which is critical to maintaining physiological homeostasis, including cell growth, development, repair, and survival. However, the understanding of autophagy in fish virus replication is limited. In this study, we found that grass carp reovirus (GCRV) infection stimulated autophagy in the spleen of grass carp (Ctenopharyngodon idella). Moreover, both Western blot (WB) analysis and fluorescent tracer tests showed that GCRV infection induced the enhancement of autophagy activation in Ctenopharyngodon idella kidney (CIK) cells. Autophagy inducer rapamycin and autophagy inhibitor 3-MA pretreatment can inhibit and promote the proliferation of GCRV, respectively. In addition, grass carp autophagy-related gene 5 (CiATG5)-induced autophagy, as well as rapamycin, showed effects on GCRV replication in CIK cells. Transcriptome analysis revealed that the total number of differentially expressed genes (DEGs) in CiATG5 overexpression groups was less than that of the control during GCRV infection. Enrichment analysis showed that CiATG5 overexpression induced the enhancement of autophagy, lysosome, phagosome, and apoptosis in the early stage of GCRV infection, which led to the clearance of viruses. In the late stage, steroid biosynthesis, DNA replication, terpenoid backbone biosynthesis, and carbon metabolism were upregulated, which contributed to cell survival. Moreover, signaling pathways involved in the immune response and cell death were downregulated in CiATG5 overexpression groups. Further study showed that CiATG5 repressed the expression of inflammatory response genes, including cytokines and type I interferons. Taken together, the results demonstrate that autophagy represses virus replication and attenuates acute inflammatory responses to protect cells.

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Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella)

Cited by 23 publications

References 38 publications

Teleost NOD-like receptors and their downstream signaling pathways: A brief review

Teleost NOD-like receptors and their downstream signaling pathways: A brief review

A New Isolate of Pediococcus pentosaceus (SL001) With Antibacterial Activity Against Fish Pathogens and Potency in Facilitating the Immunity and Growth Performance of Grass Carps

Autophagy Inhibits Grass Carp Reovirus (GCRV) Replication and Protects Ctenopharyngodon idella Kidney (CIK) Cells from Excessive Inflammatory Responses after GCRV Infection

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