30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni

Self Cite

138

Outer membrane proteins from isolates of Campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125I-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. Most isolates contained a single major band with molecular weight of 41,000 to 45,000. Profiles of C. jejuni and Campylobacter coli isolates were indistinguishable, but either could be easily differentiated from Campylobacter fetus and Campylobacter faecalis. The profiles were stable for strains under a variety of growth, incubation and passage conditions. We classified 110 isolates from patients with sporadic campylobacter enteritis into nine subtypes based on differences in outer membrane sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. Two categories accounted for 76% of the isolates. Complete concordance was observed in subtypes of strains obtained from epidemiologically related cases. Thus, comparison of the major outer membrane proteins of C. jejuni is a useful technique for investigating the transmission of this organism and may provide a basis for immunological characterization of the outer membrane proteins.

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, major questions concerning the transmission of this agent and the pathophysiology of the infection it causes remain unanswered. Recent studies have shown that isolates from humans are heterogeneous in their biochemical (13) and serological (28) characteristics.…”

mentioning

confidence: 99%

Identification and characterization of Campylobacter jejuni outer membrane proteins

Hopkins¹,

Berka²,

Vasil³

et al. 1983

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138

“…However, the limited availability of adequate strain markers has curtailed studies aimed at elucidating the sources and modes of transmission of this organism. Procedures defining unique serotypes (1,19,26,28,30), biotypes (6,18), plasmid profiles (34), and chromosomal restriction patterns (7) have all been used to identify epidemic-causing strains. The usefulness of these techniques, however, has been limited by their complexity and limited availability.…”

mentioning

confidence: 99%

Use of auxotyping for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections

Tenover¹,

Knapp²,

Patton³

et al. 1985

A chemically defined medium developed for Neisseria gonorrhoeae was modified to support the growth of Campylobacterjejuni and Campylobacter coli. A total of 76 isolates of C. jejuni and 14 isolates of C. coli were tested on this medium, which was designated Campylobacter defined medium (CDM), over a 3-month period. Although none of the C. coli isolates appeared to require amino acids, 51% of the C. jejuni tested required one and 7% required multiple amino acids for growth. An analysis of isolates obtained from three household outbreaks of campylobacteriosis demonstrated that auxotyping identified the epidemic strain within each outbreak. Among 70 isolates of C. jejuni examined, no correlation could be drawn between a specific serotype and auxotype or between auxotype and plasmid profile.

“…All Campylobacter sp. strains used in this study are shown in Table 1 and had been identified by standard criteria (7,9), passaged, and stored as previously described (16). The hippurate hydrolysis test was used to differentiate between C. jejuni and C. coli (7).…”

Section: Methodsmentioning

confidence: 99%

“…strains used in this study are shown in Table 1 and had been identified by standard criteria (7,9), passaged, and stored as previously described (16). The hippurate hydrolysis test was used to differentiate between C. jejuni and C. coli (7). Cultures were grown statically at 37°C for C. fetus or 42°C for C. jejuni and incubated in an atmosphere of 5% oxygen-10% carbon dioxide-85% nitrogen (9) for 48 h. The susceptibility of the test Campylobacter sp.…”

Section: Methodsmentioning

confidence: 99%

Antigenic heterogeneity of lipopolysaccharides from Campylobacter jejuni and Campylobacter fetus

Pérez¹,

Hopkins²,

Blaser³

1985

Self Cite

The lipopolysaccharide (LPS) structure of Campylobacter spp. can be visualized with polyacrylamide gel electrophoresis by examining proteinase K-treated whole cell lysates. Polyacrylamide gel electrophoresis LPS profiles of C. jejuni strains are rough type with low concentrations of low-molecular-weight polysaccharide side chains, serum-resistant C. fetus strains have smooth-type LPS, and serum-sensitive C. fetus strains have rough-type LPS. We electroblotted the proteinase K-treated whole cell lysates of 17 C. jejuni and 9 C. fetus strains from polyacrylamide gel electrophoresis to nitrocellulose paper to examine antigenicity to immune rabbit sera. There was virtually no antigenic cross-reactivity of C. jejuni and C. fetus LPS. Among C. jejuni strains, core LPS structures were cross-reactive, but the O-polysaccharide side chains were best recognized by homologous antisera. Antisera to several serum-resistant C. fetus strains recognized only the polysaccharide side-chain regions of serum-resistant strains and no part of the LPS from the sensitive strain. Antiserum raised against a serum-sensitive C. fetus strain but not homologous antisera recognized the core region of the LPS of the serum-resistant C. fetus strains. These findings suggest that core LPS antigens are widely shared within C. fetus subsp. fetus strains but that in the serum-resistant strains this core region is not surface exposed and therefore not immunogenic to rabbits infected with whole cells.