[30] N-Bromosuccinimide cleavage of peptides

A Novel Alkaline Esterase fromSporosarcinasp. nov. Strain eSP04 Catalyzing the Hydrolysis of a Wide Variety of Aryl-carboxylic Acid Esters

“…Thus EstAC can be said to have been fairly stable over a wide range of pH. The temperature optimum was between 40 C and 45 C, with a rapid decrease in activity at higher temperatures (Fig. 3B).…”

“…nov. strain eSP04; M (in nucleic acid), A or C; Y (in nucleic acid), C or T; PAGE, polyacrylamide gel electrophoresis having esterase activity, were examined for the hydrolysis of diethyl terephthalate (DET). They were cultured on DET minimal medium modified from Kurane et al,27) 40 mg of nicotinic acid, 40 mg of pyridoxine hydrochloride, 40 mg of thiamine hydrochloride, 40 mg of riboflavin, and 20 mg of p-aminobenzoic acid per liter (vitamins from Nacalai Tesque, Kyoto, Japan). One strain was found to form a large translucent halo around its colony through incubation for 2 d at 37…”

The primary structure of the 5 S RNA binding protein L25 from Escherichia coli ribosomes

“…The peptides derived by cleavage of the protein molecule with trypsin, with cyanogen bromide, of the modified protein with trypsin only at the arginine residues [3], as well as by cleavage of cyanogen bromide fragments with bromosuccinimide (at the tyrosine residues [4] ) were separated by gelfiltration on Sephadex G-50, chromatography on Aminex-A-5 resin, paper chromatography and paper high-voltage electrophoresis. The amino acid composition was determined on the amino acid analyzer BC-201 (Bio-Cal, BRD).…”

Effects of Fragmentation and Deamidation on the Antioxidative Activity of C Hordein.

“…NBS cleaves proteins at the carboxyl group side of tryptophan, tyrosine and histidine residues in proteins (18). In C hordein, many of the tryptophan, tyrosine and histidine residues are located in the amino terminal side and carboxyl terminal side of C hordein (19,20).…”