3-photon fluorescence and third-harmonic generation imaging of myelin sheaths in mouse digital skin <i>in vivo</i>: A comparative study

Wang, Ke; Pan, Yi; Chen, Xinlin; Tong, Sarah Y.; Liang, Huiping; Lu, Yuan; Qiu, Ping

doi:10.1142/s1793545822500031

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…1d) while limiting photobleaching [84]. Because THG signal can easily be generated in the visible spectrum from the wavelength used for 3P excitation, the combination of 3P microscopy with THG appears as a promising non-invasive tool for the multiparametric characterization of the fluorescently labeled cellular microenvironment simultaneously to the label-free imaging of the myelin coverage inside thick and diffusive tissue [85] (Fig. 1d).…”

Section: Recent Advances Of Non-linear Optical Microscopy To Improve ...mentioning

confidence: 99%

Contribution of Intravital Neuroimaging to Study Animal Models of Multiple Sclerosis

et al. 2023

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Multiple sclerosis (MS) is a complex and long-lasting neurodegenerative disease of the central nervous system (CNS), characterized by the loss of myelin within the white matter and cortical fibers, axonopathy, and inflammatory responses leading to consequent sensory-motor and cognitive deficits of patients. While complete resolution of the disease is not yet a reality, partial tissue repair has been observed in patients which offers hope for therapeutic strategies. To address the molecular and cellular events of the pathomechanisms, a variety of animal models have been developed to investigate distinct aspects of MS disease. Recent advances of multiscale intravital imaging facilitated the direct in vivo analysis of MS in the animal models with perspective of clinical transfer to patients. This review gives an overview of MS animal models, focusing on the current imaging modalities at the microscopic and macroscopic levels and emphasizing the importance of multimodal approaches to improve our understanding of the disease and minimize the use of animals.

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Section: Recent Advances Of Non-linear Optical Microscopy To Improve ...mentioning

confidence: 99%

Contribution of Intravital Neuroimaging to Study Animal Models of Multiple Sclerosis

et al. 2023

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“…Femtosecond lasers are widely used in microfabrication [1,2] , biological imaging [3][4][5][6][7] , and optical storage [8,9] due to their nonlinear optical effects. Generally, the femtosecond laser beam is focused to achieve a high peak power density for nonlinear optical effects, which means that it is only suitable for point scanning.…”

Section: Introductionmentioning

confidence: 99%

Multi-focus non-periodic scanning method for femtosecond lasers based on DMD and galvanometer scanners [Invited]

Li,

Wang,

et al. 2024

中国光学快报

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Multi-focus parallel scanning can effectively increase laser fabrication throughput. However, the conventional approach of using a spatial light modulator (SLM) to generate multi-foci and scan this fixed number of foci with galvanometer scanners can only achieve a periodic scanning trajectory due to the low switching speed of the SLM. Here we demonstrate a multifocus non-periodic scanning method for femtosecond lasers by using, instead, a fast-switching digital micromirror device (DMD) to generate a dynamic number of foci. The number of effective foci is quickly switched by introducing aberration to the undesired focus. In this way, the intensity allocated to each focus will not be affected by the number of foci, and a uniformity of 98% with different numbers of foci is achieved without adjusting the total laser energy. Finally, we validate the effectiveness of this scanning method by demonstrating corneal flap fabrication of porcine cornea in vitro.

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Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy

Zhou

Jiang

Meng

et al. 2023

J. Innov. Opt. Health Sci.

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The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery, which begins before the onset of the preterm labor process. Therefore, it is important to resolve the changes of collagen fibers during cervical remodeling for the prevention of preterm labor. Herein, we assessed the spatial organization of collagen fibers in a three-dimensional (3D) context within cervical tissues of mice on day 3, 9, 12, 15 and 18 of gestation. We found that the 3D directional variance, a novel metric of alignment, was higher on day 9 than that on day 3 and then gradually decreased from day 9 to day 18. Compared with two-dimensional (2D) approach, a higher sensitivity was achieved from 3D analysis, highlighting the importance of truly 3D quantification. Moreover, the depth-dependent variation of 3D directional variance was investigated. By combining multiple 3D directional variance-derived metrics, a high level of classification accuracy was acquired in distinguishing different periods of pregnancy. These results demonstrate that 3D directional variance is sensitive to remodeling of collagen fibers within cervical tissues, shedding new light on highly-sensitive, early detection of preterm birth (PTB).

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3-photon fluorescence and third-harmonic generation imaging of myelin sheaths in mouse digital skin in vivo: A comparative study

Cited by 7 publications

References 26 publications

Contribution of Intravital Neuroimaging to Study Animal Models of Multiple Sclerosis

Contribution of Intravital Neuroimaging to Study Animal Models of Multiple Sclerosis

Multi-focus non-periodic scanning method for femtosecond lasers based on DMD and galvanometer scanners [Invited]

Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy

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