3‐Nitrotyrosine, a marker of nitrosative stress, is increased in breath condensate of allergic asthmatic children

Baraldi, Eugenio; Giordano, Giuseppe; Pasquale, Maria Francesca; Carraro, Silvia; Mardegan, Andressa Pinto; Bonetto, G.; Bastardo, Cristina; Zacchello, Franco; Zanconato, Stefania

doi:10.1111/j.1398-9995.2006.00996.x

Cited by 54 publications

(50 citation statements)

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“…After a short course of oral steroids, MDA was reduced to levels similar to those detected in healthy children, while glutathione was increased but still lower than in healthy children [3]. In EBC of asthmatic children, we found increased levels of both 8-isoprostane [4], a product of the lipid peroxidation of arachidonic acid, and 3-nitrotyrosine [5], a product of the reaction between the potent oxidant peroxynitrate and a tyrosine residue. Although significantly higher in asthmatic than in healthy children, both these markers of oxidative stress were not different in children either treated or not treated with inhaled steroids [4,5].…”

Section: My Research As Part Of My Working Group/ Research Teamsupporting

confidence: 48%

Noninvasive markers of airway inflammation and redox biochemistry applied to asthma

Carraro¹

2006

European Respiratory Review

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At the European Respiratory Society (ERS) Annual Congress 2006, I received the ERS Annual Award for Pediatric Respiratory Research in Europe for my research work on noninvasive markers of airway inflammation. Most of the studies in which I have been involved were conducted at the University of Padova, Padova, Italy. We have studied asthmatic children, and have demonstrated that some markers of inflammation (e.g cysteinyl leukotrienes) and oxidative stress (e.g. 8-isoprostane and malondialdehyde) are increased in their exhaled breath condensate, while antioxidant products, such as glutathione, are reduced. In addition, while I was working in the laboratory of Dr Benjamin Gaston at the University of Virginia, Charlottesville, VA, USA, I conducted some basic research studies. A first group of experiments was aimed at investigating the role of rhinovirus infection in airway acidification. A second group of experiments was conducted to investigate the mechanisms involved in the regulation of expression S-nitrosoglutathione reductase (GSNOR), an enzyme that has a role in asthma pathogenesis, breaking down the endogenous bronchodilator GSNO

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Section: My Research As Part Of My Working Group/ Research Teamsupporting

confidence: 48%

Noninvasive markers of airway inflammation and redox biochemistry applied to asthma

Carraro¹

2006

European Respiratory Review

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“…(21) Although nitrotyrosine and adenosine have also been studied, neither has been shown to be a gold standard marker. (22) It is possible that a "combination of markers" (6) is required in order to differentiate asthma In a study conducted in 2007, a new device was used-the same as that used in the present study-and was shown to be extremely easy to use. (9) The device dispenses with the use of mouthpieces, special sensors, and calculations to determine study points on the EBT increase curve.…”

Section: Discussionmentioning

confidence: 94%

Temperatura do ar exalado, um novo biomarcador no controle da asma: um estudo piloto

2010

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Objective: To evaluate whether the exhaled breath temperature (EBT), measured by a noninvasive method, is an effective means of monitoring patients with uncontrolled asthma. Methods: A pilot study comprising nine patients (seven women and two men; mean age: 39 years) diagnosed with asthma at least one year prior to the beginning of the study and not having been under maintenance therapy for the last three months. In the first visit, the patients underwent spirometry and measurement of EBT. The patients were then instructed to use inhaled budesonide/formoterol (200/6 µg) every 12 h for six weeks. In addition, the patients with severe asthma (FEV 1 < 60% of predicted) were instructed to use oral prednisolone (40 mg/day) for five days. After six weeks, the patients underwent the same tests. Results: All of the patients reported an improvement in the symptoms of asthma, as confirmed by a statistically significant increase in FEV 1 from the first to the second visit (mean, 56.1% vs. 88.7% of predicted; p < 0.05). Five patients used oral prednisolone for the first five days of the treatment period. Six patients used additional doses of inhaled budesonide/formoterol (mean duration, 2.5 weeks). The EBT decreased significantly from the first to the second visit (mean EBT: 35.1°C vs. 34.1°C; p < 0.05). Conclusions: Uncontrolled asthma, especially during exacerbations, is followed by an increase in EBT, which decreases after appropriate asthma control, as demonstrated by an increase in FEV 1 and an improvement of the reported symptoms. These preliminary results suggest that EBT can be used as a parameter for the assessment of asthma control.Keywords: Asthma; Biomarkers, pharmacological; Hydroxycorticosteroids. ResumoObjetivo: Avaliar se a temperatura do ar exalado (TAE), medida por um método não invasivo, é efetiva no monitoramento de pacientes com asma não controlada. Métodos: Estudo piloto com nove pacientes (sete mulheres e dois homens; média de idade: 39 anos) com diagnóstico de asma por pelo menos um ano e sem uso de tratamento de manutenção por pelo menos três meses antes do início do estudo. Na primeira visita, os pacientes foram submetidos à espirometria e à medida da TAE. Todos os pacientes foram orientados a iniciar tratamento com budesonida/formoterol (200/6 µg) inalatório a cada 12 h por seis semanas. Além disso, os pacientes com asma grave (VEF 1 < 60% do previsto) foram orientados a utilizar prednisolona oral (40 mg/dia) por cinco dias. Após seis semanas, os pacientes foram submetidos aos mesmos testes. Resultados: Todos os pacientes relataram melhora dos sintomas de asma; confirmada por um aumento significativo de VEF 1 da primeira para a segunda visita (média de VEF 1 : 56,1% vs. 88,7% do previsto; p < 0,05). Cinco pacientes utilizaram prednisolona oral, mas somente nos cinco dias iniciais do tratamento. Seis pacientes utilizaram doses extras da medicação inalatória (média de tempo de uso de medicação adicional = 2,5 semanas). Houve uma diminuição significativa da TAE entre os momentos de avaliação ...

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“…Buss et al (2003) observed 3-chlorotyrosine in tracheal aspirate in significantly higher amounts in preterm infants with respiratory distress than in control infants. Nitrotyrosine was increased in exhaled breath condensate in patients with mild asthma (Hanazawa et al, 2000), in children (Baraldi et al, 2006) and in airway epithelial lining fluid of asthmatic children (Fitzpatrick et al 2009). …”

Section: Protein Oxidative Damagementioning

confidence: 96%

Oxidative Damage and Bronchial Asthma

Babušíková¹,

Jurečeková²,

Evinová³

et al. 2012

Respiratory Diseases

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3‐Nitrotyrosine, a marker of nitrosative stress, is increased in breath condensate of allergic asthmatic children

Abstract: Nitrotyrosine is high in EBC of asthmatic children and could be considered as a noninvasive marker of nitrosative events in the airways.

Cited by 54 publications

References 25 publications

Noninvasive markers of airway inflammation and redox biochemistry applied to asthma

Noninvasive markers of airway inflammation and redox biochemistry applied to asthma

Temperatura do ar exalado, um novo biomarcador no controle da asma: um estudo piloto

Oxidative Damage and Bronchial Asthma

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