3-Nitropropionic acid production by the endophytic Diaporthe citri: Molecular taxonomy, chemical characterization, and quantification under pH variation

Polônio, Julio Cesar; Ribeiro, Matheus Henrique Dal Molin; Rhoden, Sandro Augusto; Sarragiotto, María Helena; Azevedo, João Lúcio de; Pamphile, João Alencar

doi:10.1016/j.funbio.2016.08.006

Cited by 26 publications

(21 citation statements)

References 54 publications

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“…Based on data available in the TreeBase database (Piel et al, 2009; http://www.treebase.org), closely related species were also selected (Studies S17061 and S14881). Sequence alignment and phylogenetic analysis were performed according to Polonio et al (2016). The sequences obtained were deposited in GenBank corresponding to the ITS1-5.8S-ITS2 (accession numbers: MK804479 to MK804492), β-tubulin (MK848597 to MK848609) and GPDH (MK861119).…”

Section: Isolation Of Foliar Endophytic Fungimentioning

confidence: 99%

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Oliveira

Polli

Polônio

et al. 2020

Acta Sci. Biol. Sci.

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Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried out greenhouse experiments in bean seedlings. A high colonization frequency was obtained (89%), and the molecular identification based on DNA sequencing attested Colletotrichum as the most frequent genus and minor occurrence of Curvularia endophytes. The endophytes tested showed different types of competitive interactions in in vitro antagonism inhibition rate ranging from 28.8 to 48.8%. There were 10 promising antagonists tested for their antagonist activity of crude extracts of secondary metabolites, in which strain PE-36 (20.8%) stood out among the other strains evaluated. In the greenhouse assay, plants inoculated only with endophyte Colletotrichum sp. PE-36 was symptomless and suggest that the endophyte strengthened the growth promotion in common bean plants, especially in the root length and number of leaves when compared to control plants and other treatments. Despite many fungi of Colletotrichum genus being described as causative agents of anthracnose, in this study, the plant sampled was colonized predominantly by Colletotrichum endophytes living in asymptomatic relationship. By the way, we come across a Colletotrichum sp. endophyte able to antagonize a Colletotrichum sp. pathogen.

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Section: Isolation Of Foliar Endophytic Fungimentioning

confidence: 99%

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Oliveira

Polli

Polônio

et al. 2020

Acta Sci. Biol. Sci.

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“…The remaining 75 isolates were correctly identified at the genus level, however, 17 isolates belonging to the genera Aspergillus, Phyllosticta, Colletotrichum, Lasiodiplodia, Streptomyces, and Bacillus were misidentified at the species level (▶ Table 2) [33,[35][36][37][38][39][40].…”

Section: Problems In Dna Sequence Analysismentioning

confidence: 99%

“…(Fig. 9S) Based on phylogeny, it is not possible to identify the isolate as D. citri [37] Piper hispidum…”

Section: Kt962838mentioning

confidence: 99%

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2019

Planta Med

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“…Propionate 3‐nitronate (P3N) is a toxic nitro acid compound commonly found in ester glucosides in legumes, fungi, and leaf beetles . Upon hydrolysis by glucosidases, P3N is released from the esters and exists in a pH‐dependent equilibrium with 3‐nitropropionic acid (3‐NPA) , .…”

Section: Introductionmentioning

confidence: 99%

Crystal structure of yeast nitronate monooxygenase from Cyberlindnera saturnus

et al. 2018

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Nitronate monooxygenase (NMO) is an FMN-dependent enzyme that oxidizes the neurotoxin propionate 3-nitronate (P3N) and represents the best-known system for P3N detoxification in different organisms. The crystal structure of the first eukaryotic Class I NMO from Cyberlindnera saturnus (CsNMO) has been solved at 1.65 Å resolution and refined to an R-factor of 14.0%. The three-dimensional structures of yeast CsNMO and bacterial PaNMO are highly conserved with the exception of three additional loops on the surface in the CsNMO enzyme and differences in four active sites residues. A PEG molecule was identified in the structure and formed extensive interactions with CsNMO, suggesting a specific binding site; however, 8% PEG showed no significant effect on the enzyme activity. This new crystal structure of a eukaryotic NMO provides insight into the function of this class of enzymes.

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3-Nitropropionic acid production by the endophytic Diaporthe citri: Molecular taxonomy, chemical characterization, and quantification under pH variation

Cited by 26 publications

References 54 publications

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

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Crystal structure of yeast nitronate monooxygenase from Cyberlindnera saturnus

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