“…4-androstene-3,17-dione (AD), 1,4-androstadiene-3,17-dione (ADD) or 9a-hydroxy-4androstene-3,17-dione (9OH-AD). Thereafter, the catabolism of AD, ADD and 9OH-AD has been postulated to proceed for aerobic bacteria through a common catabolic route called 9,10-seco pathway involving two key activities D 1 -ketosteroid dehydrogenase (KSTD) and 3-ketosteroid 9a-hydroxylase (KSH) (van der Geize et al, 2001;Knol et al, 2008;Wei et al, 2010b;Fern andez de las Heras et al, 2012;Rohman et al, 2013;Petrusma et al, 2014;Yao et al, 2014). KSH is a two component class IA monooxygenase comprised of the terminal oxygenase KshA and the reductase component KshB (Andor et al, 2006;van der Geize et al, 2008;Petrusma et al, 2009;Capyk et al, 2009a,;Petrusma et al, 2011;Penfield et al, 2014) This enzyme introduces a 9a-hydroxyl group fundamental for steroid B-ring opening and thus, for its further degradation Petrusma et al, 2014).…”