3‐Hydroxypropionic acid production by recombinant <i>Escherichia coli</i> ZJU‐3HP01 using glycerol–glucose dual‐substrate fermentative strategy

Niu, Kun; Xiong, Tao; Qin, Hai-Bin; Wu, Hao; Liu, Zhiqiang; Zheng, Yu‐Guo

doi:10.1002/bab.1505

Cited by 11 publications

(10 citation statements)

References 25 publications

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“…These results altogether indicate that the codon-optimized synthetic version of the 3-HP biosynthetic pathway was correctly expressed and functional in B. subtilis . There has been a report on positive effect of glucose addition to the 3-HP producing strain of E. coli grown on glycerol, increasing the 3-HP production by reducing the imbalance between the first and second step of the pathway ( Niu et al, 2016 ). To test whether this effect applies to our strain, we grew the h-syn- KpDhaB - PuuC in two conditions: (1) 1.2% glycerol as the sole carbon source and (2) 1.2% glycerol supplemented with 1.2% glucose.…”

Section: Resultsmentioning

confidence: 99%

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Kalantari

Chen

et al. 2017

Front. Microbiol.

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3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories, focusing mainly on Escherichia coli, Klebsiella pneumoniae, and Saccharomyces cerevisiae. Despite the significant progress made in this field, commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study, we investigated the potential of Bacillus subtilis as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant B. subtilis strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from K. pneumoniae. Genetic engineering, driven by in silico optimization, and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L, in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in B. subtilis. With this relatively high titer in batch, and the robustness of B. subtilis in high density fermentation conditions, we expect that our production strains may constitute a solid basis for commercial production of 3-HP.

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Section: Resultsmentioning

confidence: 99%

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Kalantari

Chen

et al. 2017

Front. Microbiol.

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“…The addition of glucose as a co-substrate was also found to improve the activity of the aldehyde dehydrogenase gene, and in turn, improve the 3HP production yields [36]. Niu et al [36] reported a 3.5-fold increase in the activity of the enzyme, which improved the 3HP production in flasks from 3.39 g/L (control-no addition of glucose) to 6.80 g/L. Optimization of the glucose concentration and feeding strategy improved the 3HP production to up to 17.2 g/L during fed-batch cultivation.…”

Section: Engineering Of E Coli Cells For the Conversion Of Glycerol mentioning

confidence: 95%

“…This strategy resulted in the engineering of an E. coli strain capable of producing up to 56.4 g/L 3HP in a fed-batch bioreactor, with the addition of glucose together with glycerol. The addition of glucose as a co-substrate was also found to improve the activity of the aldehyde dehydrogenase gene, and in turn, improve the 3HP production yields [36]. Niu et al [36] reported a 3.5-fold increase in the activity of the enzyme, which improved the 3HP production in flasks from 3.39 g/L (control-no addition of glucose) to 6.80 g/L.…”

Section: Engineering Of E Coli Cells For the Conversion Of Glycerol mentioning

confidence: 99%

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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Abstract:The production of high added-value chemicals from renewable resources is a necessity in our attempts to switch to a more sustainable society. 3-Hydroxypropionic acid (3HP) is a promising molecule that can be used for the production of an important array of high added-value chemicals, such as 1,3-propanediol, acrylic acid, acrylamide, and bioplastics. Biological production of 3HP has been studied extensively, mainly from glycerol and glucose, which are both renewable resources. To enable conversion of these carbon sources to 3HP, extensive work has been performed to identify appropriate biochemical pathways and the enzymes that are involved in them. Novel enzymes have also been identified and expressed in host microorganisms to improve the production yields of 3HP. Various process configurations have also been proposed, resulting in improved conversion yields. The intense research efforts have resulted in the production of as much as 83.8 g/L 3HP from renewable carbon resources, and a system whereby 3-hydroxypropionitrile was converted to 3HP through whole-cell catalysis which resulted in 184.7 g/L 3HP. Although there are still challenges and difficulties that need to be addressed, the research results from the past four years have been an important step towards biological production of 3HP at the industrial level.

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“…This allowed reducing the accumulation of fermentation by-products (lactate and acetate). The benefits of adding glucose as a substrate were also verified by Niu et al [74]. AldH (KGSADH) activity was enhanced in the presence of glucose.…”

Section: Glycerol Routementioning

confidence: 67%

“…The glycerol route (CoA-independent) is probably the route most studied as 3-HP can be produced directly from glycerol in two steps (Figure 3) [36,52,[67][68][69][70]. Additionally, 3-HP can also be produced from glucose using glycerol as an intermediary [32,34,35,38,39,44,45,47,51,[71][72][73][74]. In this case, the carbon flux is often redirected to glycerol.…”

Section: Glycerol Routementioning

confidence: 99%

Heterologous Production of Acrylic Acid: Current Challenges and Perspectives

Rodrigues

2022

SynBio

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Acrylic acid (AA) is a chemical with high market value used in industry to produce diapers, paints, adhesives and coatings, among others. AA available worldwide is chemically produced mostly from petroleum derivatives. Due to its economic relevance, there is presently a need for innovative and sustainable ways to synthesize AA. In the past decade, several semi-biological methods have been developed and consist in the bio-based synthesis of 3-hydroxypropionic acid (3-HP) and its chemical conversion to AA. However, more recently, engineered Escherichia coli was demonstrated to be able to convert glucose or glycerol to AA. Several pathways have been developed that use as precursors glycerol, malonyl-CoA or β-alanine. Some of these pathways produce 3-HP as an intermediate. Nevertheless, the heterologous production of AA is still in its early stages compared, for example, to 3-HP production. So far, only up to 237 mg/L of AA have been produced from glucose using β-alanine as a precursor in fed-batch fermentation. In this review, the advances in the production of AA by engineered microbes, as well as the hurdles hindering high-level production, are discussed. In addition, synthetic biology and metabolic engineering approaches to improving the production of AA in industrial settings are presented.

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3‐Hydroxypropionic acid production by recombinant Escherichia coli ZJU‐3HP01 using glycerol–glucose dual‐substrate fermentative strategy

Cited by 11 publications

References 25 publications

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Heterologous Production of Acrylic Acid: Current Challenges and Perspectives

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