3-hydroxymorphinan enhances mitochondrial biogenesis and adipocyte browning through AMPK-dependent pathway

Jung, Tae Woo; Hwang, Eui Jin; Pyun, Do Hyeon; Kim, Tae Jin; Lee, Hyun Jung; El‐Aty, A. M. Abd; Bang, Joon Seok; Kim, Hyoung‐Chun; Jeong, Ji Hoon

doi:10.1016/j.bbrc.2021.08.083

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Excessive accumulation of lipids in adipocytes results in increased levels of intracellular ROS, which can lead to mitochondrial dysfunction by damaging proteins, membrane lipids, and nucleic acids [ 60 ]. It has been reported that both concentrated extract of Prunus mume fruit and 3-hydroxymorphinan can inhibit ROS generation in 3T3-L1 adipocytes to improve mitochondrial function [ 38 , 61 ]. Similarly, the results of the present study indicated that OFFE reduced intracellular ROS levels in a dose- and time-dependent manner, shielding mitochondria from oxidative-stress-induced harm and thereby preserving mitochondrial function.…”

Section: Discussionmentioning

confidence: 99%

Osmanthus fragrans Flavonoid Extract Inhibits Adipogenesis and Induces Beiging in 3T3-L1 Adipocytes

Yang,

Lu,

et al. 2024

Foods

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Osmanthus fragrans has a long history of cultivation in Asia and is widely used in food production for its unique aroma, which has important cultural and economic values. It is rich in flavonoids with diverse pharmacological properties, such as antioxidant, anti-tumor, and anti-lipid activities. However, little is known regarding the effects of Osmanthus fragrans flavonoid extract (OFFE) on adipogenesis and pre-adipocyte transdifferentiation. Herein, this research aimed to investigate the effect of OFFE on the differentiation, adipogenesis, and beiging of 3T3-L1 adipocytes and to elucidate the underlying mechanism. Results showed that OFFE inhibited adipogenesis, reduced intracellular reactive oxygen species levels in mature adipocytes, and promoted mitochondrial biogenesis as well as beiging/browning in 3T3-L1 adipocytes. This effect was accompanied by increased mRNA and protein levels of the brown adipose-specific marker gene Pgc-1a, and the upregulation of the expression of UCP1, Cox7A1, and Cox8B. Moreover, the research observed a dose-dependent reduction in the mRNA expression of adipogenic genes (C/EBPα, GLUT-4, SREBP-1C, and FASN) with increasing concentrations of OFFE. Additionally, OFFE activated the AMPK signaling pathway to inhibit adipogenesis. These findings elucidate that OFFE has an inhibitory effect on adipogenesis and promotes browning in 3T3-L1 adipocytes, which lays the foundation for further investigation of the lipid-lowering mechanism of OFFE in vivo in the future.

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Section: Discussionmentioning

confidence: 99%

Osmanthus fragrans Flavonoid Extract Inhibits Adipogenesis and Induces Beiging in 3T3-L1 Adipocytes

Yang,

Lu,

et al. 2024

Foods

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“…The BMSCs that were treated with PF or left untreated for 10 days were washed with PBS and incubated with DMEM containing 100 nM MitoTracker Green FM (Beyotime Biotechnology, Jiangsu, China) for 30 min at 37 °C [ 35 ]. Cells were washed with PBS and then incubated in a prewarmed DMEM medium at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Leaf Extract of Perilla frutescens (L.) Britt Promotes Adipocyte Browning via the p38 MAPK Pathway and PI3K-AKT Pathway

Chen

et al. 2023

Nutrients

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The leaf of Perilla frutescens (L.) Britt (PF) has been reported to negatively affect adipocyte formation, inhibit body-fat formation, and lower body weight. However, its effect on adipocyte browning remains unknown. Thus, the mechanism of PF in promoting adipocyte browning was investigated. The ingredients of PF were acquired from the online database and filtered with oral bioavailability and drug-likeness criteria. The browning-related target genes were obtained from the Gene Card database. A Venn diagram was employed to obtain the overlapped genes that may play a part in PF promoting adipocyte browning, and an enrichment was analysis conducted based on these overlapped genes. A total of 17 active ingredients of PF were filtered, which may regulate intracellular receptor-signaling pathways, the activation of protein kinase activity, and other pathways through 56 targets. In vitro validation showed that PF promotes mitochondrial biogenesis and upregulates brite adipocyte-related gene expression. The browning effect of PF can be mediated by the p38 MAPK pathway as well as PI3K-AKT pathway. The study revealed that PF could promote adipocyte browning through multitargets and multipathways. An in vitro study validated that the browning effect of PF can be mediated by both the P38 MAPK pathway and the PI3K-AKT pathway.

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3-hydroxymorphinan enhances mitochondrial biogenesis and adipocyte browning through AMPK-dependent pathway

Cited by 2 publications

References 27 publications

Osmanthus fragrans Flavonoid Extract Inhibits Adipogenesis and Induces Beiging in 3T3-L1 Adipocytes

Osmanthus fragrans Flavonoid Extract Inhibits Adipogenesis and Induces Beiging in 3T3-L1 Adipocytes

Leaf Extract of Perilla frutescens (L.) Britt Promotes Adipocyte Browning via the p38 MAPK Pathway and PI3K-AKT Pathway

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