3-Hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) is highly associated with the cell division during the early stage of fruit development which determines the final fruit size in Litchi chinensis

Xia, Rui; Li, Caiqin; Lu, Wei; Du, Juan; Wang, Zehuai; Li, Jianguo

doi:10.1016/j.gene.2012.01.079

Cited by 12 publications

(4 citation statements)

References 56 publications

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“…The expression level of LcHMGR1 is higher, and expression lasts longer in larger fruits. LcHMGR2 shows an expression peak during the late stages of fruit development and is related to the biosynthesis of isoprenoid substances required for cell elongation during that time (Rui et al, 2012). Previous studies have shown that NtHMGR2 is a stress-responsive gene (Hemmerlin et al, 2004; Merret et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…LcHMGR1 is most highly expressed during the early stages of fruit development and is involved in the regulation of fruit size. The level of LcHMGR1 expression is higher and lasts longer in larger fruits, while LcHMGR2 is expressed most highly during the late stages of fruit development and is related to the biosynthesis of isoprenoid substances required for cell elongation during that time (Rui et al, 2012). The expression of CaHMGR1 is temporary and tissue-specific,…”

Section: Characterization and Evolutionary History Of Hmgrmentioning

confidence: 99%

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Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Movahedi

Wei

Pucker

et al. 2020

Preprint

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Plants use two distinct isoprenoid biosynthesis pathways: the methylerythritol phosphate (MEP) pathway and mevalonic acid (MVA) pathway. 1-deoxy-D-xylulose5-phosphate synthase (DXS) and 1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR) are the rate-limiting enzymes in the MEP pathway, and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a key regulatory enzyme in the MVA pathway. Previously, overexpression of Populus trichocarpa PtDXR in Nanlin 895 poplar was found to upregulate resistance to salt and drought stresses, and the transgenic poplars showed improved growth. In the present study, PtHMGR overexpressors (OEs) exhibited higher expression levels of DXS, DXR, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS), and 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) and lower expression levels of 2-C-methyl-d-erythritol4-phosphate cytidylyltransferase (MCT), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK), and 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS) than non-transgenic poplars (NT). However, the poplar PtDXR-OEs showed upregulated expression levels of MEP-related genes and downregulated expression of MVA-related genes. Moreover, overexpression of PtDXR and PtHMGR in poplars caused changes in MVA-derived trans-zeatin-riboside (TZR), isopentenyl adenosine (IPA), castasterone (CS) and 6-deoxocastasterone (DCS), as well as MEP-derived carotenoids, gibberellins (GAs), and abscisic acid (ABA). In PtHMGR-OEs, greater accumulation of geranyl diphosphate synthase (GPS) and geranyl pyrophosphate synthase (GPPS) transcript levels in the MEP pathway led to accumulation of MEP-derived isoprenoids, while upregulation of farnesyl diphosphate synthase (FPS) expression in the MVA pathway contributed to increased levels of MVA-derived isoprenoids. Similarly, in PtDXR-OEs, increased GPS and GPPS transcript levels in the MEP pathway boosted MEP-derived isoprenoid levels and changes in FPS expression affected MVA-derived isoprenoid yields. From these results, we can conclude that cross-talk exists between the MVA and MEP pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Characterization and Evolutionary History Of Hmgrmentioning

confidence: 99%

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Movahedi

Wei

Pucker

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…The moisture content was determined with the KERN moisture analysers (MRS120-3, Kern&Sohn GmbH, German). The crude protein, total lipid, ash and crude fibre content were estimated by the Association of Official Analytical Chemists (AOAC) methods [3] The total sugar content was obtained according to the method described by Bian et al [4] using spectrophotometric method. Minerals.…”

Section: Methodsmentioning

confidence: 99%

Determination of Chemical Composition, Nutritional Ingredients and Bioactive Compounds of Three Cultivars of Mango Seeds

Huang

Shao

et al. 2014

AMR

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Three principal mango cultivars (Yuexi No.1, Tainong No.1 and Guifei) grown in Guangdong province in China were selected, and their chemical composition, nutritional ingredients including fatty acid and amino acid, and bioactive compounds such as phenolics and flavonoids were characterized and compared. The results showed the mango seeds had high moisture content (64.74-71.84%) and crude fibre content (50.86-60.62%), and the seeds of Yuexi No.1 had the lowest content of protein (1.21%), total lipid (1.47%), total sugar (2.79%), and highest content of Ca (537.02 mg/100 g), Na (22.60 mg/100 g), Zn (6.13 mg/100 g), Fe (17.02 mg/100 g) compared with the other two cultivars. The total amino acid (AA) in Guifei seeds (4.33%) was the highest, but the proportion of essential AA was the lowest. Seven fatty acid compounds were found in the three varieties. Moreover, Tainong No.1 had highest content of total phenolics (10.82%) followed with Guifei (1.86%) and Yuexi No.1 (1.06%), while the flavonoid content of Tainong No.1 was 2.71%, higher than Yuexi No.1 (0.37%) and Guifei (0.70%).

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“…The 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR), which catalyzes the conversion of HMG-CoA to MVA, is a rate-limiting enzyme in isoprenoid biosynthesis via MVA pathway (Rui et al, 2012;Dai et al, 2011). In view of its significance in isoprenoid metabolism, genes encoding HMGRs have been isolated and extensively characterized from many plants including Withania somnifera (Akhtar et al, 2013), Coffea arabica (Pleschberger et al, 2011), Paris fargesii (Liang et al, 2014), Ginkgo biloba (Liao et al, 2015) and Camptotheca acuminata (Burnett et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression Analysis of 3-Hydroxy-3-Methylglutaryl-CoA Reductase Gene from Matricaria chamomilla

Tao¹,

Liu²,

Chang³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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Chamomile (Matricaria chamomilla L.) is renowned for its production of essential oils, which major components are sesquiterpenoids. As the important enzyme in the sesquiterpenoid biosynthesis pathway, the enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catelyzes the first committed step of isoprenoids biosynthesis in MVA pathway. In this study, a full-length cDNA encoding HMGR (designated as McHMGR, GenBank Accession No. KX925561) was successfully isolated from leaves of M. chamomilla by PCR. The full-length cDNA of McHMGR was 2276 bp long and contained a 1743 bp open reading frame (ORF) encoding a 580-amino-acid protein. The sequence of the McHMGR protein was highly homologous to those of HMGR proteins from other plant species. Phylogenetic tree analysis indicated that McHMGR shared the same ancestor in evolution with HMGRs and had a further relationship with other Dicotyledoneae species. qRT-PCR analysis revealed that the expression level of McHMGR is highest in the flowers and lowest in the stems. The isolation and characterization of McHMGR gene will be helpful to further study the role of McHMGR gene in the biosynthesis of sesquiterpenoids on M. chamomilla.

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3-Hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) is highly associated with the cell division during the early stage of fruit development which determines the final fruit size in Litchi chinensis

Cited by 12 publications

References 56 publications

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Determination of Chemical Composition, Nutritional Ingredients and Bioactive Compounds of Three Cultivars of Mango Seeds

Cloning and Expression Analysis of 3-Hydroxy-3-Methylglutaryl-CoA Reductase Gene from Matricaria chamomilla

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