3-dimensional bioprinting for tissue engineering applications

Gu, Bon Kang; Choi, Duck Joo; Park, Sang Jun; Kim, Min Sup; Kang, Chang Mo; Kim, Chun-Ho

doi:10.1186/s40824-016-0058-2

Cited by 201 publications

(104 citation statements)

References 74 publications

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“…The most desirable scaffolds include interconnected porosity for fluid transport, biochemical properties that support cell function, and a diversity of physical and mechanical properties that can be customized for specific biological or medical needs. Recent advanced manufacturing approaches, such as 3-D printing or decellularization of animal tissues, have produced scaffolds with biomimetic [1] or unique physical properties [2] . Despite these advances, the ability to manufacture biomaterials with a diverse range of achievable physical and biological properties remains a challenge and an active area of inquiry [3] .…”

mentioning

confidence: 99%

Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture

Fontana

Gershlak

Adamski

et al. 2017

Adv Healthcare Materials

132

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The commercial success of tissue engineering products requires efficacy, cost effectiveness and the possibility of scale-up. Advances in tissue engineering require increased sophistication in the design of biomaterials, often challenging our current manufacturing techniques. Interestingly, several of the properties that are desirable for biomaterials design are embodied in the structure and function of plants. This study demonstrates that decellularized plant tissues can be used as adaptable scaffolds for culture of human cells. With simple biofunctionalization technique it’s possible to enable adhesion of human cells on a diverse set of plant tissues. The elevated hydrophilicity and excellent water transport abilities of plant tissues allows cell expansion over prolongued periods of culture. Moreover, cells are able to conform to the microstructure of the plant frameworks, resulting in cell alignment and pattern registration. In conclusion, the current study shows that it is feasible to use plant tissues as an alternative feedstock of scaffolds for mammalian cells.

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mentioning

confidence: 99%

Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture

Fontana

Gershlak

Adamski

et al. 2017

Adv Healthcare Materials

132

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show abstract

“…The major concerns in selecting a suitable 3D bioprinter include pressure applied on the bioink, time required for dispensing the bioink, viscosity of the bioink, post‐gelation strategy, crosslinking methods, and so on, which are extremely critical for the successful synthesis of artificial tissues or organs. The common bioprinting techniques used for the deposition and patterning of biological materials using different bioink designs are extrusion based, inkjet bioprinting, laser‐assisted bioprinting, and stereolithography (SLA; Figure ), each having its specific applications, advantages, flaws, and/or limitations (Gu et al, ; Mandrycky, Wang, Kim, & Kim, ; Wang, Ao, et al, ).…”

Section: D Bioprinting Technologiesmentioning

confidence: 99%

Advances in three‐dimensional bioprinting of bone: Progress and challenges

Midha

Dalela

Sybil

et al. 2019

J Tissue Eng Regen Med

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Several attempts have been made to engineer a viable three‐dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re‐establishing the 3D dynamic micro‐environment of the native bone. Inspite of their in vitro success, maintaining the viability and differentiation potential of such cell‐laden constructs overtime, and their subsequent preclinical testing in terms of stability, mechanical loading, immune responses, and osseointegrative potential still needs to be explored. Progress is slow due to several challenges such as but not limited to the choice of ink used for cell encapsulation, optimal cell source, bioprinting method suitable for replicating the heterogeneous tissues and organs, and so on. Here, we summarize the recent advancements in bioprinting of bone, their limitations, challenges, and strategies for future improvisations. The generated knowledge will provide deep insights on our current understanding of the cellular interactions with the hydrogel matrices and help to unravel new methodologies for facilitating precisely regulated stem cell behaviour.

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“…The mask-image-projection process employs a digital light processing technique (DLP) for the generation of a defi ned mask image [58,70,71]. The DLP system utilizes an image generation device such as the digital micro mirror device, which is effi cient in the solidifi cation of one entire 2D layer by a single projection of the pattern image [27].…”

Section: Stereolithography-based Bioprintingmentioning

confidence: 99%

“…Fused deposition modeling or fused fi lament fabrication is a thermo-based tissue engineering technique for scaffold fabrication [66]. In this technique, fi laments are heated up to their melting points which are the main source materials for the construction of 3D structures [70]. The extrusion nozzle is employed for the deposition of heated fi laments [70].…”

Section: Extrusion-based Bioprintingmentioning

confidence: 99%

3D Bioprinting: An attractive alternative to traditional organ transplantation

Iram¹,

Riaz²,

Iqbal³

2019

Arch Biomed Sci Eng

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3D bioprinting is computer-aided technology used to generate 3D models of organs. Employing this technique, organ and tissues are generated according to the patient body. 3D structures are formed by the deposition of bioink. This bioink can be natural or synthetic bionink. For in vitro implantation, the tissue is fi rst incubated in a bioreactor, however, in in vivo there is no prerequisite incubation required, rather cells are directly implanted. Bioprinting consists of various steps involving imaging, design approach, choice of material, cell selection and printing of tissue construct.3D bioprinting has two main approaches, i.e. cellular and a-cellular. Cellular bioprinting can be inkjet based, stereolithography based, laser induced forward transfer (LIFT) and extrusion based. Acellular bioprinting is extrusion based and laser based. Tissues of various organs are formed using 3D bioprinting involving blood vessels, bone, cartilage, heart, kidneys, and that of the skin and neurons. However, bioprinting of micro organs and the selection of suitable bioink is a diffi cult task. Bioprinting has various limitations that lead to the development of 4D bioprinting. This review paper will help you to understand the basic technique of 3D bioprinting, its application, limitations and new advancements that help to enhance the effi cacy of this technique.

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3-dimensional bioprinting for tissue engineering applications

Cited by 201 publications

References 74 publications

Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture

Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture

Advances in three‐dimensional bioprinting of bone: Progress and challenges

3D Bioprinting: An attractive alternative to traditional organ transplantation

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