3-D printed compact and cost-effective lens-less digital inline holographic microscopy setup for bio-imaging applications

Abstract

The applicability of the proposed 3-D printed lens-less digital inline holographic microscopy setup is demonstrated with bio-imaging examples. The direct object illumination with an elliptical beam from laser diode avoids the spatial filtering and beam collimation assembly which significantly reduces the setup complexity and cost. The use of Raspberry Pi camera sensor for hologram recording further reduces the cost without compromising on the hologram reconstructed image quality. The adjustable magnification in the range of 1.1X-1.8X is achieved. We believe that the proposed DIHM setup can be an important imaging tool especially in the resource constrained applications.