3‐Acetonyl‐3‐hydroxyoxindole: a new inducer of systemic acquired resistance in plants

Li, Yanmei; Zhang, Zhiqing Philippe; Jia, Yantao; Shen, Yuemao; He, Hongping; Fang, Rongxiang; Chen, Xiaoying; Hao, Xiao‐Jiang

doi:10.1111/j.1467-7652.2008.00322.x

Cited by 69 publications

(43 citation statements)

References 25 publications

(23 reference statements)

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“…In addition, PR-1a gene expression persisted longer in the Dufulin treatment group than that in the SA and BTH treatment groups. Moreover, a full set of methods that combine proteome technology and bioinformatics was created to identify the target of the drug [28]–[30]. To our knowledge, this is the first example of a study combining aspects of modern biotechnology, namely proteomics and bioinformatics, and molecular biology to find a drug’s target of action against a plant viral disease.…”

Section: Discussionmentioning

confidence: 99%

Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco

et al. 2012

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BackgroundDufulin is a new antiviral agent that is highly effective against plant viruses and acts by activating systemic acquired resistance (SAR) in plants. In recent years, it has been used widely to prevent and control tobacco and rice viral diseases in China. However, its targets and mechanism of action are still poorly understood.Methodology/Principal FindingsHere, differential in-gel electrophoresis (DIGE) and classical two-dimensional electrophoresis (2-DE) techniques were combined with mass spectrometry (MS) to identify the target of Dufulin. More than 40 proteins were found to be differentially expressed (≥1.5 fold or ≤1.5 fold) upon Dufulin treatment in Nicotiana tabacum K326. Based on annotations in the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, these proteins were found to be related to disease resistance. Directed acyclic graph (DAG) analysis of the various pathways demonstrated harpin binding protein-1 (HrBP1) as the target of action of Dufulin. Additionally, western blotting, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and real time PCR analyses were also conducted to identify the specific mechanism of action of Dufulin. Our results show that activation of HrBP1 triggers the salicylic acid (SA) signaling pathway and thereby produces antiviral responses in the plant host. A protective assay based on lesion counting further confirmed the antiviral activity of Dufulin.ConclusionThis study identified HrBP1 as a target protein of Dufulin and that Dufulin can activate the SA signaling pathway to induce host plants to generate antiviral responses.

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Section: Discussionmentioning

confidence: 99%

Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco

et al. 2012

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“…Natural products from plants have been proven to be a good resource in antimicrobial research, because plants have already evolved multiple mechanisms to selectively suppress pathogens by production of secondary metabolites with antimicrobial activities. 3-Acetonyl-3-hydroxyoxindole (AHO), a derivative of isatin, has been isolated from extracts of Strobilanthes cusia 8 . AHO induces resistance in tobacco plants against infection with tobacco mosaic virus (TMV), the fungal pathogen Erysiphe cichoracearum (powdery mildew) and tospoviruses 9 , possibly through the salicylic acid pathway-mediated SAR 8 .…”

Section: Introductionmentioning

confidence: 99%

Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N. tabacum

Chen¹,

Dong²,

Bennetzen

et al. 2017

Sci Rep

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3-Acetonyl-3-hydroxyoxindole (AHO) induces systemic acquired resistance (SAR) in Nicotiana. However, the underlying molecular mechanism is not well understood. To understand the molecular regulation during SAR induction, we examined mRNA levels, microRNA (miRNA) expression, and their regulatory mechanisms in control and AHO-treated tobacco leaves. Using RNA-seq analysis, we identified 1,445 significantly differentially expressed genes (DEGs) at least 2 folds with AHO treatment. The DEGs significantly enriched in six metabolism pathways including phenylpropanoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis for protective cuticle and wax. Key DEGs including PALs and PR-10 in salicylic acid pathway involved in SAR were significantly regulated. In addition, we identified 403 miRNAs belonging to 200 miRNA families by miRNA sequencing. In total, AHO treatment led to 17 up- and 6 down-regulated at least 2 folds (Wald test, P < 0.05) miRNAs (DEMs), respectively. Targeting analysis implicated four DEMs regulating three DEGs involved in disease resistance, including miR156, miR172f, miR172g, miR408a, SPL6 and AP2. We concluded that both mRNA and miRNA regulation enhances AHO-induced SAR. These data regarding DEGs, miRNAs, and their regulatory mechanisms provide molecular evidence for the mechanisms involved in tobacco SAR, which are likely to be present in other plants.

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“…In recent years, a series of natural products from plants and synthesised chemical compounds with anti-TMV activities had been reported, such as seco -pregnane steroids [3], 3-acetonyl-3-hydroxyoxindole [4], 7-deoxy- trans -dihydronarciclasine (DDN) [5], eudesmanolides [6], quassinoids [7], sesquiterpenoids [8], phenanthroindolizidines and their analogues [9], β-carbolines alkaloids [10] and their derivatives [11]. Among those compounds, DDN attracted our attention.…”

Section: Introductionmentioning

confidence: 99%

Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

Yang

Zhaorong

Chen

et al. 2018

Nat. Prod. Bioprospect.

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Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional half-leaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco.Graphical Abstract Electronic supplementary materialThe online version of this article (10.1007/s13659-018-0163-0) contains supplementary material, which is available to authorized users.

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3‐Acetonyl‐3‐hydroxyoxindole: a new inducer of systemic acquired resistance in plants

Cited by 69 publications

References 25 publications

Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco

Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco

Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N. tabacum

Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

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