3,5,3′-Triiodo-L-Thyronine Induces Cardiac Myocyte Differentiation but Not Neuronal Differentiation in P19 Teratocarcinoma Cells in a Dose-Dependent Manner

Rodríguez, E. René; Tan, Cheng-Fang; Onwuta, U. S.; Yu, Zu‐Xi; Ferrans, Víctor J.; Parrillo, Joseph E.

doi:10.1006/bbrc.1994.2715

Cited by 16 publications

(8 citation statements)

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“…The latter strategy was more effi cient with respect to the myogenic yield. T3 applied alone during P19 cell aggregation was reported to be cardiomyogenic but no indication was provided on its skeletomyogenic potential in this cell model [3]. Under conditions of the Adipo protocol, the presence of T3 in the culture medium of ES cells following RA treatment was found necessary to sustain adipogenesis but the extent to which this hormone was required for myocyte generation has induce their reprogramming to an embryonic-like state [26].…”

Section: Expression Of Oct3/4 Pluripotency Marker In Adipogenic and Omentioning

confidence: 96%

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Skeletal and Cardiac Myogenesis Accompany Adipogenesis in P19 Embryonal Stem Cells

Bouchard

Paquin

2009

Stem Cells and Development

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P19 embryonic carcinoma cells resemble normal embryonic stem (ES) cells. They generate cardiac and skeletal myocytes in response to retinoic acid (RA) or oxytocin (OT). RA treatment followed by exposure to triiodothyronine (T3) and insulin induces ES cells differentiation into adipocytes and skeletomyocytes. On the other hand, OT (10(-7) M) was reported to inhibit 3T3 preadipocyte maturation. The present work was undertaken to determine whether P19 cells have an adipogenic potential that could be affected by OT. Cells were treated with RA (10(-6) M)/T3+insulin (adipogenic protocol) or 10(-7) M OT (cardiomyogenic protocol), and analyzed by polymerase chain reaction, immunotechniques, and cytochemistry. Oil-Red-O staining and expression of peroxisome proliferator-activated receptor-gamma (PPARgamma) and aP2 indicated the generation of adipocytes in cultures submitted to the adipogenic protocol. Contracting cells were also generated. Cells positive for sarcomeric actinin and negative for cardiac troponin inhibitor (cTpnI) indicated generation of skeletomyocytes, and cTpnI positive cells revealed generation of cardiomyocytes. Levels of cTpnI and of the skeletal marker MyoD were almost similar in both protocols, whereas no Oil-Red-O staining was associated with the cardiomyogenic protocol. Addition of 10(-7) M OT to the adipogenic protocol did not affect Oil-Red-O staining and PPARgamma expression. Interestingly, Oct3/4 pluripotency marker disappeared in the adipogenic protocol but remained expressed in the cardiomyogenic one. P19 cells thus have an adipogenic potential non affected by 10(-7) M OT. RA/T3+insulin combination generates a larger spectrum of mesodermal cell derivatives and is a more potent morphogenic treatment than OT. P19 cells could help investigating mechanisms of cell fate decision during development.

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Section: Expression Of Oct3/4 Pluripotency Marker In Adipogenic and Omentioning

confidence: 96%

“…Dimethylsulfoxide, although a nonphysiological solvent, is a potent inducer [1]. Among physiologically relevant inducers are: nitric oxide [2]; steroid/vitamin molecules including retinoic acid (RA), triiodothyronine (T3), ascorbic acid [1,3,4]; the peptide hormones dynorphin, oxytocin (OT), to some extent endothelin [5][6][7][8];…”

Section: Introductionmentioning

confidence: 99%

Skeletal and Cardiac Myogenesis Accompany Adipogenesis in P19 Embryonal Stem Cells

Bouchard

Paquin

2009

Stem Cells and Development

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“…This indicates that E1A-LA could cooperate with TR to induce efficient transactivation in P19 cells. Furthermore, T 3 treatment of P19 cells induces the cells to differentiate into cardiac myocytes (41). The authors suggest that this differentiation is accompanied by preferential binding of as yet undefined factors to DR4-sites (42).…”

Section: Mol Endo · 1999mentioning

confidence: 97%

The Adenovirus E1A Protein Is a Potent Coactivator for Thyroid Hormone Receptors

Wahlström

Vennström

Bondesson

1999

Molecular Endocrinology

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The thyroid hormone receptors interact with several different cofactors when activating transciption. In this study, we show that the adenovirus E1A oncoprotein functions as a strong coactivator for the thyroid hormone receptor (TR), and that TR and E1A synergistically activate transcription via direct (DR4) or palindromic (IRO) hormone-responsive sites. Cotransfection experiments using different isoforms of the chicken TR and E1A show synergistic, ligand-enhanced transactivation. This transactivation is accomplished through a direct, ligand-independent interaction between TR and E1A. The interaction domains in TR are localized to the DNA-binding domain and to the carboxy-terminal part of the ligand-binding domain. In E1A, the regions of interactions are localized to the conserved regions 1 and 3. Both of these domains in E1A are required for a 40-fold enhancement of TR-mediated activation in transfection experiments. Taken together, we show that E1A strongly enhances transcriptional activation, which suggests that it serves as a bridging factor between the receptor and other components of the transcription machinery.

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“…To overcome this limitation, we tried to reconstitute the e ect with a continuous cell line, the pluripotential P19 mouse teratocarcinoma cells. This euploid immortalized cell line retained an ability to differentiate not only to neurons or skeletal muscle cells, depending on the inducing agent, either retinoic acid (RA), or dimethylsulfoxide, respectively (McBurney, 1993), but also into cardiac myocytes, when treated with 3,5,3'-triiodo-1-thyronine (T 3 ) (Rodriguez et al, 1994). We ®rst asked whether P19 cells, like embryonic primary neurons, express NDF receptors and the ligand itself.…”

Section: Erbb-3mentioning

confidence: 99%

Differential expression of NDF/neuregulin receptors ErbB-3 and ErbB-4 and involvement in inhibition of neuronal differentiation

et al. 1997

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Two receptor tyrosine kinases, ErB-3 and ErbB-4, mediate signaling by Neu di erentiation factors (NDFs, also called neuregulins), while ErbB-1 and ErbB-2 serve as co-receptors. We show that the two NDF/neuregulin receptors di er in spatial and temporal expression patterns: The kinase-defective receptor, ErbB-3, is expressed primarily in epithelial layers of various organs, in the peripheral nervous system, and in adult brain, whereas ErbB-4 is restricted to the developing central nervous system and to the embryonic heart. An example of alternating expression of the two receptors is provided by the developing cerebellum: During postnatal cerebellar development, ErbB-4 expression slightly decreases along with a decline in NDF transcription, whereas ErbB-3 expression commences after the peak of neurogenesis. To study functional di erences, we established primary brain cultures and found that ErbB-3 was expressed only in oligodendrocytes, whereas ErbB-4 expression was shared by oligodendrocytes, astrocytes and neurons. Blocking the action of endogenous NDF in vitro, by using a soluble form of ErbB-4, accelerated neurite outgrowth in both primary cultures and in neuronal-type cultures of the P19 teratocarcinoma, suggesting an inhibitory e ect of NDF on neural di erentiation. Apparently, ErbB-3 is associated with proliferation of P19 cells, whereas ErbB-4 correlates with a di erentiated phenotype. We conclude that the two NDF receptors play distinct, rather than redundant, developmental and physiological roles.

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3,5,3′-Triiodo-L-Thyronine Induces Cardiac Myocyte Differentiation but Not Neuronal Differentiation in P19 Teratocarcinoma Cells in a Dose-Dependent Manner

Cited by 16 publications

References 0 publications

Skeletal and Cardiac Myogenesis Accompany Adipogenesis in P19 Embryonal Stem Cells

Skeletal and Cardiac Myogenesis Accompany Adipogenesis in P19 Embryonal Stem Cells

The Adenovirus E1A Protein Is a Potent Coactivator for Thyroid Hormone Receptors

Differential expression of NDF/neuregulin receptors ErbB-3 and ErbB-4 and involvement in inhibition of neuronal differentiation

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