3,3′-Diselenodipropionic Acid, an Efficient Peroxyl Radical Scavenger and a GPx Mimic, Protects Erythrocytes (RBCs) from AAPH-Induced Hemolysis

Kunwar, Amit; Mishra, Beena; Barik, Atanu; Kumbhare, Liladhar B.; Pandey, R.; Jain, Vimal K.; Priyadarsini, K. Indira

doi:10.1021/tx700137a

Cited by 90 publications

(63 citation statements)

References 37 publications

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“…This was in accordance with our earlier studies, where it has been suggested that hemoglobin molecule being bigger in size is difficult to be released from the damaged membranes, while K + ions, being smaller in size, can be leaked even with slight disturbance in the membrane structure [27]. Since the anti-hemolytic ability of selenium compounds may be influenced by the presence of thiols, estimation of GSH levels was carried out in these samples.…”

Section: Resultssupporting

confidence: 90%

“…When compared with inorganic selenium compounds like Na 2 SeO 3 , these compounds exhibit better anti-hemolytic activity. Considering the fact from our previous studies [27] that Na 2 SeO 3 although known to exhibit better GPx activity is more toxic than any of these compounds, the present studies with organoselenium compounds gain significance.…”

Section: Discussionmentioning

confidence: 94%

“…The extent of hemolysis at different time points was determined by measuring the absorbance of the supernatant at 540 nm as described previously [27][28][29]. Similarly, membrane lipid peroxidation, K + ion loss, and GSH levels in RBCs were assessed by measuring the thiobarbituric acid reactive substances (TBARS), flame photometry (Chemito AA 203, atomic absorption spectrophotometer with programmable flame control unit), and DTNB labeling as described in our previous publications [27,28]. For reference of 100% hemolysis and K + ion leakage, a sample of RBCs was hemolyzed in distilled water.…”

Section: Evaluation Of Anti-hemolytic Activitymentioning

confidence: 99%

“…RBC membranes are very rich in polyunsaturated fatty acids, which are highly susceptible to peroxyl radical mediated peroxidation leading to leakage of hemoglobin (hemolysis), K + ions, and lowering of intracellular glutathione (GSH) levels. Therefore, the compounds have been tested for their ability to protect RBCs from peroxyl radical-induced hemolysis where the radicals have been generated by thermal decomposition of an azo compound 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) in the presence of oxygen [26][27][28]. The rate constants for the reaction of peroxyl radicals with CysSeSeCys, MeSeCys, and SeMet were estimated using competition kinetic methods.…”

Section: Introductionmentioning

confidence: 99%

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Anti-hemolytic and Peroxyl Radical Scavenging Activity of Organoselenium Compounds: An In Vitro Study

Kumar

Kunwar

Singh

et al. 2010

Biol Trace Elem Res

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Selenium-containing amino acids, selenocystine (CysSeSeCys), methylselenocysteine (MeSeCys), and selenomethionine (SeMet) have been examined for anti-hemolytic and peroxyl radical scavenging ability. Effect of these compounds on membrane lipid peroxidation, release of hemoglobin, and loss of intracellular K(+) ion as a consequence of peroxyl radicals-induced oxidation of human red blood cells were used to evaluate their anti-hemolytic ability. The peroxyl radicals were generated from thermal degradation of 2,2'-azobis(2-methylpropionamidine) dihydrochloride. Significant delay (t(eff)) was observed in oxidative damage in the presence of the selenium compounds. From the IC(50) values for the inhibition of hemolysis, lipid peroxidation, and K(+) ion leakage, the relative anti-hemolytic ability of the compounds were found to be in the order of CysSeSeCys > MeSeCys > SeMet. The anti-hemolytic abilities of the compounds, when compared with sodium selenite (Na(2)SeO(3)) under identical experimental conditions, were found to be better than Na(2)SeO(3). Relative rate constants estimated for the reaction of MeSeCys and SeMet with peroxyl radicals by competition kinetics using ABTS(2-) as a reference confirmed that all the compounds are efficient peroxyl radical scavengers. Comparison of the GPx-like activity of these compounds, by NADPH-GSH reductase coupled assay, indicated that CysSeSeCys exhibits the highest activity. Based on these results, it is concluded that among the compounds examined, CysSeSeCys, possessing the ability to reduce peroxyl radicals and hydroperoxides showed efficient anti-hemolytic activity.

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Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 94%

Section: Evaluation Of Anti-hemolytic Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-hemolytic and Peroxyl Radical Scavenging Activity of Organoselenium Compounds: An In Vitro Study

Kumar

Kunwar

Singh

et al. 2010

Biol Trace Elem Res

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“…Hemolysis of RBCs is a very good model for studying free radical induced oxidative damage to membranes and to evaluate the antioxidant activity of compounds [38,39]. Erythrocytes are known as main targets of oxidative stress due to the presenting of membrane polyunsaturated fatty acids the linolenic and arachidonic acids [40].…”

Section: Antihemolytic Activitymentioning

confidence: 99%

Antioxidant Activity Assessment of Tamus Communis L. Roots

Zerargui

Baghiani

Khennouf

et al. 2016

Int J Pharm Pharm Sci

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Objective: The aim of this study was to evaluate the antioxidant activities of Tamus communis L. (T. communis) roots. This plant is used in folk medicine in the treatment of rheumatism, arthritis and lumbago. Methods:The extraction of polyphenols has been followed by the determination of total polyphenols and flavonoids. The methanol extract (MeOHE) was chromatographied on a silica gel column. The column was eluted with chloroform and then with chloroform/methanol mixtures of increasing polarity, 6 main fractions (FI--FVI)) were collected and grouped according to their TLC (Thin layer chromatography). Several methods were used to evaluate the in vitro antioxidant activity such as DPPH (2, 2'-diphenyl-1-picrylhydrazy), bleaching of β-carotene for 24 h, lipid peroxidation and anti-hemolytic activity. An in vivo approach was carried out on mice treated with CE (crud extract) at a dose of 100 mg/Kg/day for 21 consecutive days, and one group was treated with vitamin C (Vit C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Results:The results showed that the highest level of polyphenols was recorded in FII (73.143±0.009 mg GA-Eq/g extract (Gallic Acid Equivalent))/g of extract) followed by MeOHE (69.786±0.10 mg GA-Eq/g extract). Total flavonoid contents of extracts/fractions showed that MeOHE contains the highest level. Inhibition of DPPH radical showed that MeOHE (0.1187±0.025 mg/ml) and CE (0.2363±0.019 mg/ml) have the most efficient antioxidant activity. All the extracts were capable of inhibiting the bleaching of β-carotene by scavenging linoleate derived free radicals, and all the studied extracts have a very important antihemolytic activity (p<0.001) and revealed an extension of hemolysis half time from 70.34±2.15 min of CTL (AAPH (2,2-azo-bis (2-amidinopropane) dihydrochloride)) and 207±5.65 min for FI. The in vivo assay was exhibited a significant decrease (54.09%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (47.30% and 46.87% respectively). Conclusion:These findings suggest that the extract obtained from T. communis have active substances contributing to the increase in natural antioxidant potential.

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Organoselenium and organotellurium compounds: Toxicology and pharmacology

Nogueira

Rocha

2011

Patai's Chemistry of Functional Groups

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In the last three decades, the interest in the field of synthesis and reactivity of organoselenium and organotellurium compounds has increased; particularly, in view of the observations that they can exhibit important biological activities. The data presented in this chapter clearly indicate the potential pharmacological and therapeutic uses of organoselenium and organotellurium compounds. Indeed, these classes of molecules exhibit a variety of interesting biological effects, namely antioxidant properties, which can account for their in vitro and in vivo beneficial effects in a wide range of models of different human pathologies. We can conclude that the future of “medicinal chemistry“ of organoselenium and organotellurium compounds will depend on the rational development of new molecules, which can be guided by chemical and biological approaches. Moreover, the structure‐activity relationship for a given class of organoselenium or organotellurium compounds should be used as a toll for screening molecules with high probability of exhibiting low toxicity and high pharmacological activity in mammals.

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3,3′-Diselenodipropionic Acid, an Efficient Peroxyl Radical Scavenger and a GPx Mimic, Protects Erythrocytes (RBCs) from AAPH-Induced Hemolysis

Cited by 90 publications

References 37 publications

Anti-hemolytic and Peroxyl Radical Scavenging Activity of Organoselenium Compounds: An In Vitro Study

Anti-hemolytic and Peroxyl Radical Scavenging Activity of Organoselenium Compounds: An In Vitro Study

Antioxidant Activity Assessment of Tamus Communis L. Roots

Organoselenium and organotellurium compounds: Toxicology and pharmacology

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