2G1600 An optical marker based on the UV-induced green-to-red photoconverison of a novel fluorescent protein

Ando, Ryoko; Hama, Hiroya; Yamamoto-Hino, Miki; Mizuno, Hiroaki; Miyawaki, A.

doi:10.2142/biophys.42.s117_1

Cited by 11 publications

(11 citation statements)

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“…To this aim, we took advantage of the photoconvertible Kaede protein expressed under the control of the macrophage promoter mpeg1 (mpeg1::Gal4-UAS::Kaede; Ellett et al, 2011). This approach allows lineage tracing of cells in vivo, as red fluorescence remains stable over the course of several days and weeks (Ando et al, 2002;Hatta et al, 2006;Dixon et al, 2012;Tomura and Kabashima, 2013).…”

Section: Resultsmentioning

confidence: 99%

The SLC7A7 Transporter Identifies Microglial Precursors prior to Entry into the Brain

Rossi¹,

Casano²,

Henke³

et al. 2015

Cell Reports

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During development, macrophages invade organs to establish phenotypically and transcriptionally distinct tissue-resident populations. How they invade and colonize these organs is unclear. In particular, it remains to be established whether they arise from naive equivalents that colonize organs randomly or whether there are committed macrophages that follow pre-determined migration paths. Here, by using a combination of genetics and imaging approaches in the zebrafish embryo, we have addressed how macrophages colonize the brain to become microglia. Identification and cloning of a mutant that lacks microglia has shown that Slc7a7, a Leucine/Arginine transporter, defines a restricted macrophage sub-lineage and is necessary for brain colonization. By taking a photoconversion approach, we show that these macrophages give rise to microglia. This study provides direct experimental evidence for the existence of sub-lineages among embryonic macrophages.

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Section: Resultsmentioning

confidence: 99%

The SLC7A7 Transporter Identifies Microglial Precursors prior to Entry into the Brain

Rossi¹,

Casano²,

Henke³

et al. 2015

Cell Reports

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“…C57BL/6J were originally obtained from the Jackson Laboratory (Bar Harbor, ME). B6.Cg-Tg(CAG-tdKaede)15Utr mice (Ando et al, 2002;Tomura et al, 2008) were bred from pairs obtained from Dr. Tatyana Chtanova (Garvan Institute of Medical Research, Australia). All experiments were approved by the independent animal ethical committee ''Ethische Commissie Dierproeven -faculteit Geneeskunde en Gezondheids-wetenschappen Universiteit Gent.''…”

Section: Star+methods Key Resources Tablementioning

confidence: 99%

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection

et al. 2020

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“…Proteins were expressed in Escherichia coli (BL21[DE3] or JM109[DE3]) and purified using Ni 2+ or glutathione-affinity chromatography as described previously (Ando et al, 2002). In the latter case, UnaG protein was obtained from GST-UnaG protein adsorbed on glutathione Sepharose 4B (GE Healthcare) by thrombin (GE Healthcare) digestion.…”

Section: Apounag Protein Expression In Bacteriamentioning

confidence: 99%

A Bilirubin-Inducible Fluorescent Protein from Eel Muscle

Kumagai

Ando

Miyatake

et al. 2013

Cell

282

389

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The fluorescent protein toolbox has revolutionized experimental biology. Despite this advance, no fluorescent proteins have been identified from vertebrates, nor has chromogenic ligand-inducible activation or clinical utility been demonstrated. Here, we report the cloning and characterization of UnaG, a fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is triggered by an endogenous ligand, bilirubin, a membrane-permeable heme metabolite and clinical health biomarker. The holoUnaG structure at 1.2 Å revealed a biplanar coordination of bilirubin by reversible π-conjugation, and we used this high-affinity and high-specificity interaction to establish a fluorescence-based human bilirubin assay with promising clinical utility. UnaG will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering.

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2G1600 An optical marker based on the UV-induced green-to-red photoconverison of a novel fluorescent protein

Cited by 11 publications

References 0 publications

The SLC7A7 Transporter Identifies Microglial Precursors prior to Entry into the Brain

The SLC7A7 Transporter Identifies Microglial Precursors prior to Entry into the Brain

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection

A Bilirubin-Inducible Fluorescent Protein from Eel Muscle

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