2D Spectroscopy Study of Water-Soluble Chlorophyll-Binding Protein from Lepidium virginicum

Abstract

Water-soluble chlorophyll-binding proteins (WSCPs) are interesting model systems for the study of pigment-pigment and pigment-protein interactions. While class IIa WSCP has been extensively studied by spectroscopic and theoretical methods, a comprehensive spectroscopic study of class IIb WSCP was lacking so far despite the fact that its structure was determined by X-ray crystallography. In this paper, results of two-dimensional electronic spectroscopy applied to the class IIb WSCP from Lepidium virginicum are presented. Global analysis of 2D data allowed determination of energy levels and excitation energy transfer pathways in the system. Some additional pathways, not present in class IIa WSCP, were observed. The data were interpreted in terms of a model comprising two interacting chlorophyll dimers. In addition, oscillatory signals were observed and identified as coherent beatings of vibrational origin.

Mapping energy transfer channels in fucoxanthin–chlorophyll protein complex

“…Temporal evolution of the spectrum can conveniently be represented by different sorts of decay associated spectra (DAS). Standard DAS analysis of the 2D spectra [36][37][38], which is similar to the global analysis of the pump-probe spectra [39], is a powerful tool to find out the timescales of the dominant processes in the system. However, it assumes the same decay constants for all data points in spectrum and, thus, it is not suitable for resolving the differences in dynamics upon excitation of Fx red or Fx blue in the 2D spectra of FCP.…”

Assembly of water-soluble chlorophyll-binding proteins with native hydrophobic chlorophylls in water-in-oil emulsions

“…Most of the spectroscopic research so far focused on class IIa WSCPs from cauliflower [12][13][14][15] instead of the class IIb WSCP from Virginia pepperweed for which high resolution data is available. Only recently, Alster et al presented a 2D spectroscopy study of WSCP from Virginia pepperweed [16].…”

Probing environment fluctuations by two-dimensional electronic spectroscopy of molecular systems at temperatures below 5 K

“…73 Additionally, its 2DES spectra at 77 K showed vibrational beats. 43 Phonon spectral density of this system has been experimentally determined 36,37,44 and is used here to study a model dimer. Experimental and theoretical HB and 2DES data of the recombinant tetramer LvWSCP and CaWSCP will be reported elsewhere.…”