2009
DOI: 10.1186/1471-2180-9-198
|View full text |Cite
|
Sign up to set email alerts
|

2D proteome analysis initiates new Insights on the SalmonellaTyphimurium LuxS protein

Abstract: BackgroundQuorum sensing is a term describing a bacterial communication system mediated by the production and recognition of small signaling molecules. The LuxS enzyme, catalyzing the synthesis of AI-2, is conserved in a wide diversity of bacteria. AI-2 has therefore been suggested as an interspecies quorum sensing signal. To investigate the role of endogenous AI-2 in protein expression of the Gram-negative pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), we performed a 2D-DIGE proteomics exp… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
13
0
1

Year Published

2011
2011
2021
2021

Publication Types

Select...
5
3

Relationship

0
8

Authors

Journals

citations
Cited by 19 publications
(14 citation statements)
references
References 59 publications
0
13
0
1
Order By: Relevance
“…(Soni, et al, 2008) In a different report, only 4 proteins were differentially expressed, 1 of which was LuxS itself, and 2 others were unidentified proteins. (Kint, et al, 2009) The differences between the current study and the two previous proteomics-based studies of S. typhimurium are readily interpreted. For one, employing Δ luxS mutants in the previous studies compared to the use of a QS inhibitor in our study is a distinct difference.…”
Section: Discussionmentioning
confidence: 70%
“…(Soni, et al, 2008) In a different report, only 4 proteins were differentially expressed, 1 of which was LuxS itself, and 2 others were unidentified proteins. (Kint, et al, 2009) The differences between the current study and the two previous proteomics-based studies of S. typhimurium are readily interpreted. For one, employing Δ luxS mutants in the previous studies compared to the use of a QS inhibitor in our study is a distinct difference.…”
Section: Discussionmentioning
confidence: 70%
“…sphaeroides and P. denitrificans, and may be widespread in Gram-negative bacteria, but has previously not been investigated systematically or mechanistically. Other proteins lacking a signal peptide, such as LuxS in Salmonella (Kint et al, 2009) and an aberrant PhoA in Synechococcus (Ray et al, 1991), have also been detected in the periplasm, and may be targeted in a similar way. As Mn/Fe-SODs are required for successful infection by many pathogens (Hale, 1991;McGee and Mobley, 1999;Purdy et al, 1999;Verneuil et al, 2006;Kang et al, 2007) and indeed can be a major antigen (McMillan et al, 2004), this finding forces a re-evaluation of the role of SodA in pathogenesis as well as expanding on the known mechanisms of targeting proteins to the periplasm.…”
Section: Resultsmentioning
confidence: 99%
“…LuxS exhibits AI‐2‐independent activity in S . Typhimurium by modulating flagellar phase variation to favour expression of the more immunogenic phase‐1 flagellin (Karavolos et al ., ; Kint et al ., ). Formation of biofilms by S .…”
Section: Quorum Sensing and Interkingdom Communicationmentioning
confidence: 97%
“…In S. Typhimurium, AI-2 affects the expression of the Lsr system involved in its own uptake Xavier and Bassler, 2003;De Keersmaecker et al, 2005). LuxS exhibits AI-2-independent activity in S. Typhimurium by modulating flagellar phase variation to favour expression of the more immunogenic phase-1 flagellin (Karavolos et al, 2008a;Kint et al, 2009). Formation of biofilms by S. Typhimurium is dependent on the small RNA micA whose expression is affected by luxS via a yet unknown mechanism (Kint et al, 2010).…”
Section: Quorum Sensing and Interkingdom Communicationmentioning
confidence: 99%