2D HSQC Chemical Shifts of Impurities from Biomass Pretreatment

Abstract

Two dimensional (2D) heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) is a powerful analytical method which can be used to elucidate the structure of biomass. During processing, biomass is typically subjected to some form of chemical treatment which can be performed with a variety of compounds. The presence of these compounds, even in trace amounts, has the potential to contaminate the sample and lead to misinterpretation of the HSQC spectra. Here we report the chemical shifts of 29 compounds commonly used in biomass processing which have the potential to contaminate the biomass samples and lead to the misinterpretation of peaks associated with biomass (Populus trichocarpa) pretreated via autohydrolysis. The identification of these chemical shifts could serve as a valuable tool in preventing errors in characterizing biomass via HSQC.

Hydrothermal Pretreatment of Lignocellulosic Feedstocks to Facilitate Biochemical Conversion

“…Analysis using solution-state and solid-state nuclear magnetic resonance (NMR) is useful for obtaining information about the effects of pretreatment on biomass ( Lu and Ralph, 2011 ; Bryant et al, 2020 ). Solid-state NMR offers relatively low resolution and sensitivity, but has the advantage that the original structure of the biomass sample is maintained.…”