2D chromatography – Opportunities and limitations

Prototyping of a Microfluidic Modulator Chip and Its Application in Heart-Cut Strong-Cation-Exchange-Reversed-Phase Liquid Chromatography Coupled to Nanoelectrospray Mass Spectrometry for Targeted Proteomics

“…The tangible benefits of multidimensional liquid chromatography technology with respect to realizing a high peak capacity per unit time have led to a significant momentum for the two-dimensional LC (2D-LC) instrument and application development. − One of the most critical elements in 2D-LC is the interface between the first-dimension ( 1 D) and the second-dimension ( 2 D) column that collects and transfers fractions between developments. In comprehensive two-dimensional LC (LC × LC), online modulation is typically performed using a modulator consisting of a two-position 10-port switching valve equipped with two external transfer loops, but other valve configurations have also become available. − In a tutorial paper on 2D-LC, Stoll and Carr reviewed the principles of 2D-LC and showed the applicability in selected application examples .…”