“…As VEGF plays a dual role in angiogenesis initiation, vessels branching and invadosome formation (Daubon et al, 2016;Guegan et al, 2008;Seano et al, 2014aSeano et al, , 2014b, we simultaneously tested the impact of VEGF treatment on DDR2 expression and on linear invadosome formation/activity. For this assay we established four cell conditions, in presence of gelatin (a denatured form of type I collagen, not recognized by DDR2) with or without VEGF or in the presence of type I collagen (on the top of a gelatin matrix) with or without VEGF (Di Martino et al, 2017). We first measured the impact of VEGF treatment on DDR2 expression ( Figure 2A).…”