2D and 3D Hybrid Systems for Enhancement of Chondrogenic Differentiation of Tonsil‐Derived Mesenchymal Stem Cells

Park, Jinhye; Kim, In Young; Patel, Madhumita; Moon, Hye Sung; Hwang, Seong Ju; Jeong, Byeongmoon

doi:10.1002/adfm.201500299

Cited by 71 publications

(55 citation statements)

References 48 publications

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“…[11][12][13] Stem cell engineering using the graphene or chemically exfoliated graphene oxide (GO) has been investigated as one such potential biomedical application, due to its physicochemical and electrical properties that allow control of cellular behaviors (e.g., adhesion, proliferation, differentiation, polarization, and paracrine secretion) and biocompatibility. [14][15][16][17][18] Previous studies have reported enhanced adhesion, proliferation, and differentiation of mesenchymal stem cells on graphene or GO substrates based on their chemical properties and their patterning. 12,19 In addition, it has been demonstrated that the differentiation of neural stem cells (NSCs), which are sensitive to electrical signal, could be promoted on the surface of graphene or GO substrates due to their electrical properties.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide Hierarchical Patterns for the Derivation of Electrophysiologically Functional Neuron-like Cells from Human Neural Stem Cells

Yang

Lee

et al. 2016

ACS Appl. Mater. Interfaces

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Graphene has shown great potential for biomedical engineering applications due to its electrical conductivity, mechanical strength, flexibility, and biocompatibility. Topographical cues of culture substrates or tissue-engineering scaffolds regulate the behaviors and fate of stem cells. In this study, we developed a graphene oxide (GO)-based patterned substrate (GPS) with hierarchical structures capable of generating synergistic topographical stimulation to enhance integrin clustering, focal adhesion, and neuronal differentiation in human neural stem cells (hNSCs). The hierarchical structures of the GPS were composed of microgrooves (groove size: 5, 10, and 20 μm), ridges (height: 100-200 nm), and nanoroughness surfaces (height: ∼10 nm). hNSCs grown on the GPS exhibited highly elongated, aligned neurite extension along the ridge of the GPS and focal adhesion development that was enhanced compared to that of cells grown on GO-free flat substrates and GO substrates without the hierarchical structures. In particular, GPS with a groove width of 5 μm was found to be the most effective in activating focal adhesion signaling, such as the phosphorylation of focal adhesion kinase and paxillin, thereby improving neuronal lineage commitment. More importantly, electrophysiologically functional neuron-like cells exhibiting sodium channel currents and action potentials could be derived from hNSCs differentiated on the GPS even in the absence of any of the chemical agents typically required for neurogenesis. Our study demonstrates that GPS could be an effective culture platform for the generation of functional neuron-like cells from hNSCs, providing potent therapeutics for treating neurodegenerative diseases and neuronal disorders.

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Section: Introductionmentioning

confidence: 99%

Graphene Oxide Hierarchical Patterns for the Derivation of Electrophysiologically Functional Neuron-like Cells from Human Neural Stem Cells

Yang

Lee

et al. 2016

ACS Appl. Mater. Interfaces

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“…Previous reports have confirmed using graphene as a cell differentiation controlling biomaterial, especially for neurogenesis and osteogenesis on 2D substrates . GO has been considered as a carrier for therapeutic proteins due to its highly biocompatible and low cytoxicity effects . Nayak and colleagues found an uncommon behavior of osteoblast cells using graphene coated on SiO 2 /Si substrate …”

Section: Introductionmentioning

confidence: 94%

Suspended graphene oxide nanoparticle for accelerated multilayer osteoblast attachment

Foroutan

Nazemi

Tavana

et al. 2017

J Biomedical Materials Res

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Mimicking bone tissues having layered structures is still a significant challenge because of the lack of technologies to assemble osteoblast cell types into bone structures. One of the promising and attractive materials in biomedical and different engineering fields is graphene and graphenebased nanostructures such as graphene oxide (GO) because of their unique properties. In most studies, GO was synthesized using chemical vapor deposition method, and was coated on the substrate. In this study, we proposed a simple technique for assembly of cells that facilitates the construction of osteoblast-like structures using suspended GO synthesized by graphite powder, H 2 SO 4 , and KMnO 4 .Toxicity effects of GO on human mesenchymal stem cells (hMSCs) derived from bone marrow were analyzed. In addition to normal MSCs, toxicity effects of GO on human cancer cell line saos-2 as an abnormal cell line that possess several osteoblastic features, was examined. The attachment and expression of osteoblast cells genes were evaluated after differentiation of MSCs to osteoblast cells in presence of suspended GO by scanning electron microscopy and real time PCR. We found that the toxicity effects of GO are dose dependent and in oseogenic medium containing suspended GO the expression level of osteoblast genes osteopontin and osteocalcin and cell adhesion markers connexin were higher than control group. Interestingly, through this method GO was found to induce multilayer osteoblast cell morphology and enhance the number of cell layer. We expect that the presented method would become a highly useful approach for bone tissue engineering.

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“…Additionally, graphene‐containing polymer hydrogel due to its biocompatibility can be used as biosensor, imaging agent, drug delivery carrier, etc . In particular, GO is an effective component in the composite hydrogel for the adjustment of cell behaviors and biological sequences due to its special surface properties.…”

Section: Properties and Applications Of Polymer–graphene Hydrogelsmentioning

confidence: 99%

Recent Progress of Graphene‐Containing Polymer Hydrogels: Preparations, Properties, and Applications

Pan

Liu

Gai

2017

Macro Materials & Eng

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Polymer hydrogels have attracted much attention due to their mechanical, biological, and physicochemical properties. Incorporation of functional material enlarges their applications. Graphene, as a promising additive, has received great attention due to its large specific surface area, ultrahigh conductivity, strong antioxidant, thermal stability, high thermal conductivity, and good mechanical properties. In this brief review, graphene‐containing polymer hydrogels with special properties are summarized including their preparations, properties, and applications. In addition, future perspectives of polymer hydrogels containing graphene are briefly discussed.

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2D and 3D Hybrid Systems for Enhancement of Chondrogenic Differentiation of Tonsil‐Derived Mesenchymal Stem Cells

Cited by 71 publications

References 48 publications

Graphene Oxide Hierarchical Patterns for the Derivation of Electrophysiologically Functional Neuron-like Cells from Human Neural Stem Cells

Graphene Oxide Hierarchical Patterns for the Derivation of Electrophysiologically Functional Neuron-like Cells from Human Neural Stem Cells

Suspended graphene oxide nanoparticle for accelerated multilayer osteoblast attachment

Recent Progress of Graphene‐Containing Polymer Hydrogels: Preparations, Properties, and Applications

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