Performance of Treponemal Tests for the Diagnosis of Syphilis

Park, Ina U.; Fakile, Yetunde; Chow, Joan M.; Gustafson, Kathleen J.; Jost, Heather; Schapiro, Jeffrey M.; Novak-Weekley, Susan; Tran, Anthony; Nomura, Jim; Chen, Victor; Beheshti, Manie; Tsai, Townson; Hoover, Karen W.; Bolan, Gail

doi:10.1093/cid/ciy558

Cited by 87 publications

(92 citation statements)

References 19 publications

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“…Una limitación de este estudio fue el uso del método FTA-Abs como segundo ensayo treponémico, debido a su menor sensibilidad para los estadios primario y latente tardío (78-96%) 2,22 , en lugar de la prueba TPPA recomendada por el CDC y las guías actuales del Ministerio de Salud de Argentina 23 . La FTA-Abs ha sido considerada el estándar de oro como método confirmatorio 9 , debido a su adecuada especificidad y en cada caso se realizó por personal del laboratorio entrenado y con experiencia en técnicas de inmunofluorescencia indirecta.…”

Section: Discussionunclassified

Implementación del algoritmo reverso para el cribado de sífilis en un laboratorio clínico

Martínez-Methol¹,

Ventimiglia

Aristimuño³

et al. 2019

Rev. chil. infectol.

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Section: Discussionunclassified

Implementación del algoritmo reverso para el cribado de sífilis en un laboratorio clínico

Martínez-Methol¹,

Ventimiglia

Aristimuño³

et al. 2019

Rev. chil. infectol.

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“…Aufgrund der Möglichkeit zur automatisierten Durchführung kommen zunehmend polyvalente ELISA-und chemolumineszenzbasierte Tests als Suchtests zum Einsatz. Alle genannten Verfahren haben eine nahezu 100 % ige Sensitivität [12], allerdings erfolgt eine Serokonversion frühestens 2-3 Wochen nach Erregerkontakt. Bei dringendem klinischem Verdacht auf eine frische Infektion muss ggf.…”

Section: Serologieunclassified

Augenbeteiligung bei venerischer Syphilis – Update

Rath

Scheel

Kriegel

et al. 2020

Klin Monbl Augenheilkd

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ZusammenfassungSyphilis ist eine bakterielle Infektionskrankheit, die durch die Spirochäte Treponema pallidum übertragen wird. Die Zahl von Neuerkrankungen ist in den letzten Jahren kontinuierlich gestiegen. Die Augenbeteiligung ist eine wohl unterschätzte Manifestation der Syphilis, die gehäuft bei HIV-positiven Patienten auftritt. Obschon praktisch jeder Teil der Augen im Rahmen einer Syphilis betroffen sein kann, tritt sie häufig mit einer posterioren oder Panuveitis auf. Eine besonders typische Verlaufsform ist die akute syphilitische plakoide Chorioretinitis, mit typischen Charakteristika in der multimodalen Bildgebung. Bei dem Verdacht auf eine Syphilis sind treponemenspezifische und nicht treponemenspezifische serologische diagnostische Tests angezeigt. Bei frühzeitigem Einsatz einer antibiotischen Therapie (primär mit Penicillin) werden oft gute Ergebnisse hinsichtlich Morphologie und Visus erzielt. Additive Kortikosteroide sollten verlaufsorientiert und erst nach dem Beginn der Antibiotikatherapie verwendet werden.

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“…Our institution has been using the BioPlex IgG screen for our syphilis screening assay based its automation, high throughput, and ease of use. However, other syphilis screening assays are available, such as the Lumipulse G TP‐N chemiluminescent immunoassay, TrepSure EIA, and LIASON CIA, that detect both a treponemal IgM antibody in addition to an IgG antibody. Since the IgG BioPlex screening does not detect IgM treponemal, very early cases of syphilis may not be detected.…”

Section: Introductionmentioning

confidence: 99%

“…5 A number of studies have demonstrated a high percentage of falsely reactive tests using EIA/CLIA methods in the reverse syphilis screening algorithm. 3,[5][6][7] Reverse algorithm screening often results in a higher false-reactivity rate than traditional testing does in areas with both a low and high prevalence of syphilis. 3,5 In our previous study of the BioPlex IgG screen, the overall false-reactive rate was 1.0%.…”

Section: Introductionmentioning

confidence: 99%

“…8 Our institution has been using the BioPlex IgG screen for our syphilis screening assay based its automation, high throughput, and ease of use. However, other syphilis screening assays are available, such as the Lumipulse G TP-N chemiluminescent immunoassay, 9 TrepSure EIA, 6 and LIASON CIA, 9 This study is an analysis of effect on accuracy with the addition of the detection of IgM treponemal antibodies in the IgG/IgM screen compared to the IgG screen along with an analysis of the automated RPR compared with the traditional RPR assay.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the BioPlex 2200 syphilis total screen (IgG/IgM) with reflex to an automated rapid plasma reagin test

Rourk

Litwin

2019

Clinical Laboratory Analysis

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BackgroundWe evaluated the recently FDA cleared BioPlex 2200 Syphilis Total Screen and automated rapid plasma reagin (RPR) assay for the detection of total (IgG/IgM) treponemal and non‐treponemal antibodies in the reverse syphilis algorithm.MethodsProspectively submitted samples (n = 885) were assayed by both the IgG/IgM BioPlex Syphilis Screen and the original IgG BioPlex Syphilis Screen. The IgG screen reactive samples were reflexed to traditional RPR, and IgG/IgM screen reactive samples were reflexed to the automated RPR. Nonreactive RPR samples were tested by the Treponemal Pallidum Particle Agglutination test (TP‐PA). Additional samples were collected (n = 404 total samples) to directly compare the automated and traditional RPR assays with each other.ResultsThe sensitivity and specificity of the IgG/IgM screen with automated RPR was 95.6% (95% confidence interval [CI] 87.0‐99.1) and 99.6% (CI 99.2‐99.8) while the sensitivity and specificity of the BioPlex IgG screen with traditional RPR was 97.8% (CI 89.1‐99.9) and 99.3% (CI 98.8‐99.4). The sensitivity and specificity of the BioPlex RPR compared with traditional RPR was 95.8% (CI 93.9‐97.0) and 94.1% (CI 89.4‐91.1) and 95.3% (CI 92.6‐97.1). The mean of the titer differences between the BioPlex RPR and the traditional RPR was 1.0 ± 0.9 SD titers.ConclusionThe addition of the detection of treponemal IgM antibodies to the IgG/IgM screen did not significantly affect the sensitivity and specificity compared to the original IgG screen. However, the addition of the comparable BioPlex RPR assay to the instrumentation significantly reduces the overall labor of syphilis screening and confirmation.

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Performance of Treponemal Tests for the Diagnosis of Syphilis

Cited by 87 publications

References 19 publications

Implementación del algoritmo reverso para el cribado de sífilis en un laboratorio clínico

Implementación del algoritmo reverso para el cribado de sífilis en un laboratorio clínico

Augenbeteiligung bei venerischer Syphilis – Update

Evaluation of the BioPlex 2200 syphilis total screen (IgG/IgM) with reflex to an automated rapid plasma reagin test

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