Engineering copper nanoparticles synthesized on the surface of carbon nanotubes for anti-microbial and anti-biofilm applications

Seo, Youngmin; Hwang, Jang‐Sun; Lee, Eunwon; Kim, Young Jin; Lee, Kyung-Woo; Park, Chanhwi; Choi, Yonghyun; Jeon, Hojeong; Choi, Jonghoon

doi:10.1039/c8nr02768d

Cited by 66 publications

(29 citation statements)

References 108 publications

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“…Despite the fact that there is a large number of nanomaterials with intrinsic bactericidal properties, which have been reviewed elsewhere, only some nanomaterials have been shown to have the intrinsic abilities to destroy the EPS or killing bacterial biofilm [32,33]. Most of the intrinsic biofilm-eradicating nanomaterials are derived from bactericidal nanomaterials and thus they were often also demonstrated to have effects on biofilm formation as reviewed below.…”

Section: Nanomaterials With Intrinsic Biofilm-eradicating Propertiesmentioning

confidence: 99%

Nanomaterials for Treating Bacterial Biofilms on Implantable Medical Devices

Tran

Booth

et al. 2020

Nanomaterials

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Bacterial biofilms are involved in most device-associated infections and remain a challenge for modern medicine. One major approach to addressing this problem is to prevent the formation of biofilms using novel antimicrobial materials, device surface modification or local drug delivery; however, successful preventive measures are still extremely limited. The other approach is concerned with treating biofilms that have already formed on the devices; this approach is the focus of our manuscript. Treating biofilms associated with medical devices has unique challenges due to the biofilm’s extracellular polymer substance (EPS) and the biofilm bacteria’s resistance to most conventional antimicrobial agents. The treatment is further complicated by the fact that the treatment must be suitable for applying on devices surrounded by host tissue in many cases. Nanomaterials have been extensively investigated for preventing biofilm formation on medical devices, yet their applications in treating bacterial biofilm remains to be further investigated due to the fact that treating the biofilm bacteria and destroying the EPS are much more challenging than preventing adhesion of planktonic bacteria or inhibiting their surface colonization. In this highly focused review, we examined only studies that demonstrated successful EPS destruction and biofilm bacteria killing and provided in-depth description of the nanomaterials and the biofilm eradication efficacy, followed by discussion of key issues in this topic and suggestion for future development.

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Section: Nanomaterials With Intrinsic Biofilm-eradicating Propertiesmentioning

confidence: 99%

Nanomaterials for Treating Bacterial Biofilms on Implantable Medical Devices

Tran

Booth

et al. 2020

Nanomaterials

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“…Metal nanoparticles (Me-NPs) such as AuNPs, AgNPs or CuNPs display unique properties that make them suitable at surface science for the development of antimicrobial formulations. Previous studies investigated the integration of NPs in biomaterials showing unique recognition, catalytic, and inhibition properties [6,[11][12][13][14]. Especially gold or silver NPs are largely used for biomedical application since they can be used as biomarkers and drug-delivery agents with a bactericidal effect [15].…”

Section: Introductionmentioning

confidence: 99%

“…This seems to be due to the release of copper ions, which inhibited the quorum sensing in Methylobacterium spp. This resulted in inhibition of the expression of the genes that form biofilms [14].…”

Section: Introductionmentioning

confidence: 99%

Cu Nanoparticle-Loaded Nanovesicles with Antibiofilm Properties. Part I: Synthesis of New Hybrid Nanostructures

et al. 2020

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Copper nanoparticles (CuNPs) stabilized by quaternary ammonium salts are well known as antimicrobial agents. The aim of this work was to study the feasibility of the inclusion of CuNPs in nanovesicular systems. Liposomes are nanovesicles (NVs) made with phospholipids and are traditionally used as delivery vehicles because phospholipids favor cellular uptake. Their capacity for hydrophilic/hydrophobic balance and carrier capacity could be advantageous to prepare novel hybrid nanostructures based on metal NPs (Me-NPs). In this work, NVs were loaded with CuNPs, which have been reported to have a biofilm inhibition effect. These hybrid materials could improve the effect of conventional antibacterial agents. CuNPs were electro-synthesized by the sacrificial anode electrolysis technique in organic media and characterized in terms of morphology through transmission electron microscopy (TEM). The NVs were prepared by the thin film hydration method in aqueous media, using phosphatidylcholine (PC) and cholesterol as a membrane stabilizer. The nanohybrid systems were purified to remove non-encapsulated NPs. The size distribution, morphology and stability of the NV systems were studied. Different quaternary ammonium salts in vesicular systems made of PC were tested as stabilizing surfactants for the synthesis and inclusion of CuNPs. The entrapment of charged metal NPs was demonstrated. NPs attached preferably to the membrane, probably due to the attraction of their hydrophobic shell to the phospholipid bilayers. The high affinity between benzyl-dimethyl-hexadecyl-ammonium chloride (BDHAC) and PC allowed us to obtain stable hybrid NVs c.a. 700 nm in diameter. The stability of liposomes increased with NP loading, suggesting a charge-stabilization effect in a novel antibiofilm nanohybrid material.

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“…Copper oxide nanoparticles (CuO-NPs) have gained a lot of attention for industrial applications due to their beneficial optical, electrical and thermal properties [1][2][3][4]. They are frequently applied as catalysts or additives [5][6][7][8] as well as for medical purposes due to their high antimicrobial potential [9]. Copper-containing NPs are generated and released not only into the outdoor environment during welding [10] or from anti-fouling paints [11], but also in indoor environments from household electric appliances like vacuum cleaners [12].…”

Section: Introductionmentioning

confidence: 99%

Iron-Doping of Copper Oxide Nanoparticles Lowers Their Toxic Potential on C6 Glioma Cells

et al. 2020

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Copper oxide nanoparticles (CuO-NPs) are well known for their cytotoxicity which in part has been attributed to the release of copper ions from CuO-NPs. As iron-doping has been reported to reduce the susceptibility of CuO-NPs to dissolution, we have compared pure CuO-NPs and CuO-NPs that had been doped with 10% iron (CuO-Fe-NPs) for copper release and for their toxic potential on C6 glioma cells. Physicochemical characterization revealed that dimercaptosuccinate (DMSA)-coated CuO-NPs and CuO-Fe-NPs did not differ in their size or zeta potential. However, the redox activity and liberation of copper ions from CuO-Fe-NPs was substantially slower compared to that from CuO-NPs, as demonstrated by cyclic voltammetry and by the photometric quantification of the copper ion-bathocuproine complex, respectively. Exposure of C6 cells to these NPs caused an almost identical cellular copper accumulation and each of the two types of NPs induced ROS production and cell toxicity. However, the time-and concentration-dependent loss in cell viability was more severe for cells that had been treated with CuO-NPs compared to cells exposed to CuO-Fe-NPs. Copper accumulation and toxicity after exposure to either CuO-NPs or CuO-Fe-NPs was prevented in the presence of copper chelators, while neutralization of the lysosomal pH by bafilomycin A1 prevented toxicity without affecting cellular copper accumulation or ROS production. These data demonstrate that iron-doping does not affect cellular accumulation of CuO-NPs and suggests that the intracellular liberation of copper ions from CuO-NPs is slowed by the iron doping, which in turn lowers the cell toxic potential of iron-doped CuO-NPs.

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Engineering copper nanoparticles synthesized on the surface of carbon nanotubes for anti-microbial and anti-biofilm applications

Cited by 66 publications

References 108 publications

Nanomaterials for Treating Bacterial Biofilms on Implantable Medical Devices

Nanomaterials for Treating Bacterial Biofilms on Implantable Medical Devices

Cu Nanoparticle-Loaded Nanovesicles with Antibiofilm Properties. Part I: Synthesis of New Hybrid Nanostructures

Iron-Doping of Copper Oxide Nanoparticles Lowers Their Toxic Potential on C6 Glioma Cells

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