Multigenerational Effects and Demographic Responses of Zebrafish (Danio rerio) Exposed to Organo-Bromine Compounds

Abstract

Long-term exposure to toxic chemicals often has deleterious effects on aquatic organisms. In order to support appropriate environmental management of chemicals, a mathematical model was developed to characterize the effects of chemicals on multigenerational population dynamics in aquatic animals. To parametrize the model, we conducted a multigenerational laboratory toxicity test in zebrafish ( Danio rerio) exposed to 2-bromo-4,6-dinitroaniline (BDNA). Long-term exposure to BDNA considerably reduced the fecundity of adult zebrafish (F and F) and caused deformities in the offspring (F). Life history data, including changes in fecundity and population growth, were then integrated into the model to predict population dynamics of zebrafish exposed to two novel brominated flame retardants, bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (TBPH) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB). The model predicted that the fecundity of adult zebrafish would be significantly impaired after exposure to 90.36 μM TBPH and 99.16 μM TBB. Thus, prolonged exposure to such levels over multiple generations could result in population extinction within 20 years. Our results provide an intensive temporal perspective to investigate a keystone that connects with individual response to chemicals, population dynamics, and ultimately ecosystem influences.

Enhanced Nontarget Screening Algorithm Reveals Highly Abundant Chlorinated Azo Dye Compounds in House Dust

“…Previous studies have reported the mutagenicity and reproductive toxicities of brominated azo dyes, ,,,− and it is likely that chlorinated azo dyes follow similar toxicity pathways. Thus, chlorinated azo dyes are of concern, given their higher abundances than brominated analogues.…”

Toxicokinetics of Brominated Azo Dyes in the Early Life Stages of Zebrafish (Danio rerio) Is Prone to Aromatic Substituent Changes

“…Indeed, the mutagenicity of BDNA has been reported with the Ames (TA98) assay at a relatively low concentration (1.25 μg/mL) . In vivo aquatic toxicities of BDNA also revealed its impacts on zebrafish reproductivity and development after long-term exposure to BDNA at a concentration as low as 0.5 μg/L. , Thus, investigation of the potential bioactivation of BADs, for instance, to produce BDNA is critical to understand their potential adverse effects.…”