Inhibition of α-ketoglutarate dehydrogenase activity affects adventitious root growth in poplar via changes in GABA shunt

Yue, Jianyun; Du, Changjian; Ji, Jing; Xie, Ting; Chen, Wei; Chang, Ermei; Chen, Lanzhen; Jiang, Zeping; Shi, Shengqing

doi:10.1007/s00425-018-2929-3

Cited by 27 publications

(27 citation statements)

References 45 publications

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“… 50 Similarly, a high GABA content also inhibits adventitious root growth. For example, adventitious root growth of poplar is inhibited if the GABA level is increased in poplar lines by inhibition of α-ketoglutarate dehydrogenase activity , 51 overexpression of PagGAD2 52 or exogenous GABA application. 51 , 52 The changes in GABA shunt activity affect cell-wall carbon metabolism-related genes and phytohormone (IAA, ABA, and ethylene) signaling.…”

Section: Gaba Acts As a Signal Molecule In Plantsmentioning

confidence: 99%

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The versatile GABA in plants

Dou

Zhang

et al. 2021

Plant Signaling & Behavior

168

100

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Gamma-aminobutyric acid (GABA) is a ubiquitous four-carbon, non-protein amino acid. GABA has been widely studied in animal central nervous systems, where it acts as an inhibitory neurotransmitter. In plants, it is metabolized through the GABA shunt pathway, a bypass of the tricarboxylic acid (TCA) cycle. Additionally, it can be synthesized through the polyamine metabolic pathway. GABA acts as a signal in Agrobacterium tumefaciens -mediated plant gene transformation and in plant development, especially in pollen tube elongation (to enter the ovule), root growth, fruit ripening, and seed germination. It is accumulated during plant responses to environmental stresses and pathogen and insect attacks. A high concentration of GABA elevates plant stress tolerance by improving photosynthesis, inhibiting reactive oxygen species (ROS) generation, activating antioxidant enzymes, and regulating stomatal opening in drought stress. The transporters of GABA in plants are reviewed in this work. We summarize the recent research on GABA function and transporters with the goal of providing a review of GABA in plants.

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Section: Gaba Acts As a Signal Molecule In Plantsmentioning

confidence: 99%

“… 51 , 52 The changes in GABA shunt activity affect cell-wall carbon metabolism-related genes and phytohormone (IAA, ABA, and ethylene) signaling. 51 , 52 …”

Section: Gaba Acts As a Signal Molecule In Plantsmentioning

confidence: 99%

The versatile GABA in plants

Dou

Zhang

et al. 2021

Plant Signaling & Behavior

168

100

View full text Add to dashboard Cite

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“…Similarly, exogenous GABA inhibits primary root growth in Arabidopsis (Renault et al 2011). Recently, it was reported that the inhibition of AR growth by blocking α-ketoglutarate dehydrogenase (α-KGDH) was accompanied by changes in GABA shunt (Yue et al 2018). In poplar (Populus alba × P. glandulosa), endogenous GABA accumulation not only inhibited the AR growth, but also delayed AR primordia formation and the AR appearance within one day.…”

Section: Introductionmentioning

confidence: 99%

γ-Aminobutyric Acid Participates in the Adult-Phase Adventitious Rooting Recalcitrance

Shen

et al. 2020

J Plant Growth Regul

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In many tree species especially the rooting-recalcitrant woody perennials, the adventitious root (AR) in the juvenile phase can be easily induced by exogenous auxin, but AR formation becomes recalcitrant in the adult phase. Also, it is reported that the γ-aminobutyric acid (GABA) inhibits primary root growth in Arabidopsis and the AR formation in poplar (Populus ssp). So far, how GABA affects or is affected by the ontogenetic phase or auxin remains unclear. Here, we used an apple rootstock, Malus xiaojinensis, and tobacco (Nicotiana benthamiana) to investigate this question. We first analyzed the content of GABA, the activity of GABA synthetic enzyme GAD, and the expression of the coding gene MxGADs, respectively, in leafy cuttings of juvenile and adult phase. Next, the effect of exogenous GABA on AR formation was examined in in vitro shoots of M. xiaojinensis and tobacco. Interestingly, significant and consistent increases in GABA concentration, GAD activity, and expression of MxGAD genes in response to exogenous indole butyric acid (IBA) were detected in adult-phase cuttings, but not in juvenile-phase cuttings. Exogenous GABA application inhibited the AR formation by delaying rooting time and reducing root number and the total root length in in vitro shoots of both M. xiaojinensis and tobacco. The expression of MxPIN members increased in response to IBA application, but these changes were restrained by the addition of GABA. These results indicate that both the loss of juvenility and IBA are required to trigger GABA accumulation. GABA may affect the AR formation as a co-actor by inhibiting polar auxin transport. Together, these findings facilitate the understanding of the regulatory network among GABA, juvenility, and auxin signaling on the AR formation.

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“…Detection of GABA content in the wild-type and mutant seedlings was modified from previously reported methods ( Liu et al, 2018 ; Yue et al, 2018 ). Briefly, 0.1 g of the wild-type lap2-3 and cat2-1 mutant seedlings were ground with liquid nitrogen to a fine powder and extracted using 1 ml acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Mutual Promotion of LAP2 and CAT2 Synergistically Regulates Plant Salt and Osmotic Stress Tolerance

Zhang

Wang

Li³

et al. 2021

Front. Plant Sci.

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Almost all abiotic stresses induce reactive oxygen species (ROS) overaccumulation, causing oxidative damages to plant cells. Catalase (CAT) plays a vital role in plant oxidative stress tolerance by scavenging stress-induced excess H2O2; thus, the identification of factors regulating catalase function will shed light on the underlying regulatory mechanisms. Here, we identified leucine aminopeptidase 2 (LAP2) as a novel CAT2-interacting protein and showed a mutual promotion effect of the two proteins in plant stress responses. LAP2 has a physical interaction with CAT2 in plant cells. The loss-of-function mutant of LAP2, lap2-3, is hypersensitive to salt or osmotic stress with increased ROS accumulation and malondialdehyde content and decreased catalase activity. The lap2-3 mutant has less CAT2 protein levels as CAT2 protein stability is impaired in the mutant. Scavenging excess ROS by glutathione or overexpressing CAT2 in the lap2-3 mutant recovers its hypersensitive phenotype to salt or osmotic stress. Further study showed that CAT2 promotes LAP2 hydrolysis activity with leucine-4-methylcoumaryl-7-amides as a substrate in vivo and in vitro, and thus, similar to the lap2-3 mutant, the cat2-1 mutant also has lower γ-aminobutyric acid content than the wild type. Together, our study reveals mutual promotion effects of CAT2 and LAP2 in conferring plant salt and osmotic stress tolerance.

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Inhibition of α-ketoglutarate dehydrogenase activity affects adventitious root growth in poplar via changes in GABA shunt

Cited by 27 publications

References 45 publications

The versatile GABA in plants

The versatile GABA in plants

γ-Aminobutyric Acid Participates in the Adult-Phase Adventitious Rooting Recalcitrance

Mutual Promotion of LAP2 and CAT2 Synergistically Regulates Plant Salt and Osmotic Stress Tolerance

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