Transfection Mediated by DEAE-Dextran

Abstract

Here, we describe two variations on the classical DEAE-dextran transfection procedure. The first involves a brief exposure of cells to a high concentration of DEAE-dextran and yields higher transfection frequencies but elevated cellular toxicity. The second involves a longer exposure of cells to a lower concentration of DEAE-dextran, which produces lower transfection frequencies but increased cell survival.

Introducing Genes into Cultured Mammalian Cells

“…When using more exotic lines of cells, it is important to compare the efficiencies of several different methods. Protocol: Transfection Mediated by DEAE-Dextran (Kumar et al 2018d…”