Effect of allogeneic blood transfusion on levels of IL‑6 and sIL‑R2 in peripheral blood of children with acute lymphocytic leukemia

Abstract

Effect of allogeneic blood transfusion on the expression of interleukin-6 (IL-6) and soluble interleukin-2 receptor (sIL-2R) in peripheral blood of children with acute lymphoblastic leukemia (ALL) was investigated. A total of 91 ALL children admitted to Nanfang Hospital from June 2014 to January 2017 were selected as the study group. Patients were randomly divided into allogeneic blood transfusion group (n=38) and non-transfusion group (n=53). In addition, a total of 64 healthy children were also selected from June 2014 to January 2017 as the control group. Patients in allogeneic blood transfusion group were transfused with red blood cell suspension and machine-collected platelets, while patients in non-transfusion group were not treated with blood transfusion. Peripheral venous blood was collected before and at 4, 8 and 12 weeks after blood transfusion to prepare serum. Serum IL-6 and sIL-2R levels were measured by enzyme-linked immunosorbent assay (ELISA). Before transfusion, serum levels of IL-6 and sIL-2R were significantly lower in the study group than those in control group (p<0.05), and no significant differences in serum levels of IL-6 and sIL-2R were found between the allogeneic blood transfusion and non-transfusion group. After transfusion, serum levels of IL-6 and sIL-2R were stable for 12 weeks in the non-transfusion group, while IL-6 and sIL-2R levels were significantly increased in the allogeneic blood transfusion group. The results showed that serum level of IL-6 and sIL-2R was increased in ALL patients with allogeneic blood transfusion, which resulted in reduced antibody production and decreased cellular immunity. The patients had low immunity, and attention should be paid on the pathogen infection prevention.

Evaluation of Interleukin-6 Level Before Chemotherapy in Acute Lymphoblastic Leukemia L1 Standard-Risk and High-risk Patients

“…The results of this study also found an increase in IL-6 levels, which were quite high in both the HR and SR groups, proving the role of IL-6 in the pathogenesis of ALL as in the study by Luo et al,Zhao et al,and Farsani et al [13], [14], [2] Therefore, it is necessary to consider the administration of anti-IL-6 as in the study [15]. In addition, the increase in IL-6 levels in both groups was also caused by an infection in both groups, as in the research conducted by Xu XJ [16].…”

High soluble interleukin-2 receptor values in Indian paediatric & adult controls – Need for population-specific threshold in the diagnosis of haemophagocytic lymphohistiocytosis

“…However, Japanese controls had a lower serum sIL2R in children and in adults with ages similar to our series 7 . In China, Gao et al 8 too reported low levels of sIL2R in children aged 2-3 yr. Two reports used the same manufacturer as the present study, Zhao et al 9 described serum sIL2R in Chinese children aged 3-12 yr similar to the present study, while Deveci et al 10 reported considerably lower sIL2R than the present study 9 10 ( Table V ). Thus, both children and adults from India seem to have higher serum sIL2R concentrations than most reports.…”

Regulation of Macrophage Polarization by miR-449a/Cripto-1-PI3K/AKT/NF-κB Signaling Pathway in Allogeneic Transfusion Mice

“…Allogeneic transfusion induces immune dysfunction in blood recipients by nonspecific and specific immunosuppression. The mechanism is generally believed that the inhibition of nonspecific immunity includes the inhibition of neutrophils, macrophages, natural killer cells, cytokines, and complement system [6,7]. Macrophages are one of the important components of the innate immune system.…”