Rapid Confirmation of the Zaire Ebola Virus in the Outbreak of the Equateur Province in the Democratic Republic of Congo: Implications for Public Health Interventions

Mbala-Kingebeni, Placide; Villabona-Arenas, Christian Julián; Vidal, Nicole; Likofata, Jacques; Nsio-Mbeta, Justus; Makiala-Mandanda, Sheila; Mukadi, Daniel; Mukadi, Patrick; Kumakamba, Charles; Djokolo, Bathe; Ayouba, Ahidjo; Delaporte, Éric; Peeters, Martine; Tamfum, Jean‐Jacques Muyembe; Ahuka‐Mundeke, Steve

doi:10.1093/cid/ciy527

Cited by 41 publications

(33 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Public Health Emergency of International Concern (PHEIC) recently called for an extraordinary public health response, similar to what was observed for SARS, H1N1, Zaire Ebolavirus, and Zika [11][12][13][14]. However, the COVID-19 outbreak targeted a large number of locations during a short period of time, resulting in increasing concerns about the speed and mode of transmission of the disease, particularly taking into consideration the large number of medical staff and the general population infected in Wuhan [15].…”

Section: Introductionmentioning

confidence: 78%

The psychological impact of COVID-19 outbreak on medical staff and the general public

Chen

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Purpose: To assess the psychological effects of the novel coronavirus disease (COVID-19) on medical staff and the general public.Methods: During the outbreak of COVID-19, an internet-based questionnaire included The Self-rating Depression Scale (SDS), Perceived Stress Scale (PSS-10), and Impact of Event Scale-Revised (IES-R) was used to assess the impact of the epidemic situation on the mental health of medical staff and general population in Wuhan and its surrounding areas.Results: The results suggest that the outbreak of COVID-19 has affected individuals significantly, the degree of which is related to age, sex, occupation and mental illness. There was a significant difference in PSS-10 and IES-R scores between the medical staff and the general population. The medical staff showed higher PSS-10 scores (16.813 ± 4.87) and IES-R scores (22.40 ± 12.12) compared to members of the general population PSS-10 (14.80 ± 5.60) and IES-R scores (17.89 ± 13.08). However, there was no statistically significant difference between the SDS scores of medical staff (44.52 ± 12.36) and the general public (43.08 ± 11.42). In terms of the need for psychological assistance, 50.97% of interviewees responded that they needed psychological counseling, of which medical staff accounted for 65.87% and non-medical staff accounted for 45.10%.Conclusion: During the ongoing COVID-19 outbreak, great attention should be paid to the mental health of the population, especially medical staff, and measures such as psychological intervention should be actively carried out for reducing the psychosocial effects.

show abstract

Section: Introductionmentioning

confidence: 78%

The psychological impact of COVID-19 outbreak on medical staff and the general public

Chen

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, this article puts forward a set of small molecule-induced perturbation of interfacial electrostatic interaction network within experimentally determined ebola GP structures, and hypothesizes that these small compounds inhibit viral cell entry and/or membrane-fusion by binding to the glycoprotein, inducing a minor conformational perturbation, leading to the establishment of two inter-chain salt bridges, i.e., B_LYS_510-A_GLU_292 ( Figures 2, 3 and 4) and B_LYS_588-A_ASP_47 ( Figures 5, 6 and 7), restraining the conformational flexibility of the approximate structural region in the orange box (Figures 2 and 3) and the approximate structural region in the red box ( Figure 5) against GP-mediated Ebolavirus cell entry and/or membrane-fusion [14][15][16][115][116][117][118]. Along with previously reported details of the protein-inhibitor interactions of these complexes, this hypothesis may facilitate the design of more potent inhibitors of Ebolavirus cell entry [119][120][121][122][123][124][125]125,[144][145][146][147][148][149] and the discovery of novel antiviral compounds [54,[150][151][152][153][154][155][156] to prepare us for future outbreaks.…”

Section: Conclusion and Discussionmentioning

confidence: 58%

Two Achilles' Heels of the Ebolavirus Glycoprotein?

Li¹

2020

Preprint

View full text Add to dashboard Cite

Ebolavirus has a membrane envelope decorated by trimers of a glycoprotein (GP), which is responsible for host cell attachment and membrane fusion. Therefore, GP is a primary target for antiviral drugs development. Here, this article reports the first, to my knowledge, set of structural analysis of all Ebolavirus GP structures as of March 10, 2020, and also a brief update of the structurally identified electrostatic features of the Ebolavirus GP structures in both apo (unliganded) state and also in bound states with a series of small compounds, including a variety of approved drugs. With this comprehensive set of structural analysis, this article puts forward a hypothesis of two Achilles' heels of Ebolavirus GP structure, where the formation of two interfacial salt bridges, instead of destabilizing the prefusion conformation of Ebolavirus GP, constitutes a positive contribution towards the structural rigidification of the prefusion conformation of the Ebolavirus GP structure, thereby acting against GP-mediated Ebolavirus cell entry and/or preventing fusion between the viral and endosome membranes.

show abstract

“…The MinION sequencing protocol described here followed the manufacturer’s recommended protocol, using the standard 48-hour script in order to maximise read depth. As demonstrated elsewhere (31), an alternative approach is to run the MinION for a shorter amount of time, flush the flow cell and add different samples. Larger barcode kits (up to 96) and custom barcodes can also be used.…”

Section: Discussionmentioning

confidence: 99%

Extraction-free direct PCR from dried serum spots permits HBV genotyping and RAS identification by Sanger and minION sequencing

Astbury

Mmd

Peprah³

et al. 2019

Preprint

View full text Add to dashboard Cite

Word count (3,000 limit; excluding abstract, Methods, References, Tables and Figure 19Legends): 2967 20 regional/national centres to provide information relevant to patient management. By 36 providing viable workflows for both the Sanger and MinION sequencing platforms, which 37 vary greatly in the infrastructure and expertise required, we demonstrate that MinION 38 sequencing is a viable method for HBV genotyping in resource-limited settings. These 39 workflows could also be applied to sequencing of other blood borne DNA viruses and 40 bacterial pathogens. 41 Abstract word count: 230 42 43 44

show abstract

Rapid Confirmation of the Zaire Ebola Virus in the Outbreak of the Equateur Province in the Democratic Republic of Congo: Implications for Public Health Interventions

Cited by 41 publications

References 7 publications

The psychological impact of COVID-19 outbreak on medical staff and the general public

The psychological impact of COVID-19 outbreak on medical staff and the general public

Two Achilles' Heels of the Ebolavirus Glycoprotein?

Extraction-free direct PCR from dried serum spots permits HBV genotyping and RAS identification by Sanger and minION sequencing

Contact Info

Product

Resources

About